FastQCFastQC Report
Thu 26 May 2016
SRR1547665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547665_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2668962
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118670.4446297849126365No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43920.16455835639473324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94900.041.6332971
TACGGGA6900.040.1086964
TTTAGCG2100.038.571431
ACGGGTA3750.038.3999985
TATTGCG2150.037.674421
TCGGTTA301.1403266E-437.49999624
TACGTCC601.5643309E-1037.49999610
AGGGCGA32550.037.188946
TACGAAT2300.037.17391212
GGACTAA20200.037.0915878
CGTAAGG4950.036.818182
TATAACG552.752131E-936.818181
TAAGGGA24000.036.6562544
ACGGGAT7250.036.620695
ATAGGGC19000.036.3552634
TAGGGTA16750.036.134335
GGGCGAT67850.036.1127477
GTCGAAC250.002107606536.013
GCCGGAT504.883077E-836.019
CGTTCGG5400.035.83333645
Produced by FastQC (version 0.11.5)