FastQCFastQC Report
Thu 26 May 2016
SRR1547664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547664_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4010921
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440451.0981268391972816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT378000.043.4880941
TACGGGA10700.038.9018674
GTTTTTT434100.038.4018672
TACGGGT7450.038.053694
AGGGCGA48500.037.809286
ACGGGTA8000.037.68755
TATGGGC22250.037.4157334
TAGGGCG22850.037.122545
GGGCGAT94350.036.891897
CAACGCG552.752131E-936.818181
CGTTAGG7300.036.678082
ATAGGGC27550.036.2613454
CGAGGGA17500.036.257144
TGGGCGA25850.036.0348176
TACGTCG250.002107733236.01
GACCGAT26100.035.9482779
TAAGGGT32150.035.7620544
ATAGGGT31750.035.7165374
TAGGGCA31600.035.6012655
TAGGGTA29650.035.362565
Produced by FastQC (version 0.11.5)