FastQCFastQC Report
Thu 26 May 2016
SRR1547663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547663_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2826105
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211780.7493706001723219No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG207.0350355E-445.02
CGTTTTT263600.043.6855051
GTTTTTT301700.038.720582
TCGTTAG1050.038.571431
TCGATAG2100.038.571431
AGGGCGA32450.038.204936
CAGTACG659.094947E-1238.0769271
TAACGGG8000.037.6874963
GGGCGAT63350.037.4348877
GCGATAT4650.037.258069
TAGGGAC19650.036.297715
GGCGATA14600.036.2157558
TACGGGT4300.036.1046524
TAGGGTG25000.036.095
ACGGGTA5050.036.089115
TATGGGC17650.036.0764854
ACGATAC250.002107628235.99999638
CGCGTAA250.002107628235.99999631
CGTTAGG3950.035.8860782
AGTACGG2900.035.689662
Produced by FastQC (version 0.11.5)