##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547663_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2826105
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431267061910297	31.0	31.0	33.0	30.0	34.0
2	31.853046153628405	31.0	31.0	34.0	30.0	34.0
3	31.92000474150819	31.0	31.0	34.0	30.0	34.0
4	35.66812768810784	37.0	35.0	37.0	33.0	37.0
5	35.578635259482574	37.0	35.0	37.0	33.0	37.0
6	35.63720633168265	37.0	35.0	37.0	33.0	37.0
7	36.04239439086658	37.0	35.0	37.0	35.0	37.0
8	36.063712423989905	37.0	35.0	37.0	35.0	37.0
9	37.82724668757884	39.0	38.0	39.0	35.0	39.0
10	37.12491998704931	39.0	37.0	39.0	33.0	39.0
11	36.940091044034105	39.0	37.0	39.0	33.0	39.0
12	36.56104284872643	39.0	35.0	39.0	32.0	39.0
13	36.45841255013526	39.0	35.0	39.0	32.0	39.0
14	37.55249079563569	40.0	36.0	41.0	32.0	41.0
15	37.67433481770847	40.0	36.0	41.0	33.0	41.0
16	37.70590760074378	40.0	36.0	41.0	33.0	41.0
17	37.63871653742518	40.0	36.0	41.0	33.0	41.0
18	37.43949216324234	39.0	36.0	41.0	32.0	41.0
19	37.29552971315645	39.0	36.0	41.0	32.0	41.0
20	37.095143315623446	39.0	35.0	41.0	32.0	41.0
21	37.01076711587149	38.0	35.0	41.0	32.0	41.0
22	36.92335281243974	38.0	35.0	41.0	32.0	41.0
23	36.81557833130758	38.0	35.0	41.0	32.0	41.0
24	36.71399505680079	38.0	35.0	40.0	31.0	41.0
25	36.59017057044944	38.0	35.0	40.0	31.0	41.0
26	36.4521534054821	38.0	35.0	40.0	31.0	41.0
27	36.40751175204035	38.0	35.0	40.0	31.0	41.0
28	36.39866565467313	38.0	35.0	40.0	31.0	41.0
29	36.3238283786342	38.0	35.0	40.0	30.0	41.0
30	36.2926476546342	38.0	35.0	40.0	30.0	41.0
31	36.088503788783505	38.0	35.0	40.0	30.0	41.0
32	35.7137544429524	38.0	35.0	40.0	29.0	41.0
33	35.348865311090705	38.0	34.0	40.0	25.0	41.0
34	34.89105288020084	38.0	34.0	40.0	23.0	41.0
35	34.60505890616237	38.0	34.0	40.0	21.0	41.0
36	34.40830471620835	38.0	34.0	40.0	20.0	41.0
37	34.140730793795704	38.0	33.0	40.0	18.0	41.0
38	34.19583879579846	38.0	33.0	40.0	18.0	41.0
39	34.06864571556966	38.0	33.0	40.0	18.0	41.0
40	33.95408981619579	38.0	33.0	40.0	18.0	41.0
41	33.89268657746262	38.0	33.0	40.0	18.0	41.0
42	33.857576417012105	37.0	33.0	40.0	18.0	41.0
43	33.78410143996773	37.0	33.0	40.0	17.0	41.0
44	33.638433462309436	37.0	33.0	40.0	17.0	41.0
45	33.596623267712985	37.0	33.0	40.0	17.0	41.0
46	33.42735390227893	37.0	33.0	40.0	16.0	41.0
47	33.43927101080816	37.0	33.0	40.0	17.0	41.0
48	33.262152326258224	36.0	33.0	40.0	15.0	41.0
49	33.242294960732174	36.0	33.0	40.0	15.0	41.0
50	33.106660580551676	36.0	32.0	40.0	15.0	41.0
51	32.43236503951552	35.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	1.0
12	1.0
13	13.0
14	32.0
15	108.0
16	224.0
17	698.0
18	1465.0
19	3164.0
20	5621.0
21	9174.0
22	13970.0
23	21222.0
24	33404.0
25	53464.0
26	73444.0
27	74398.0
28	64845.0
29	61912.0
30	67537.0
31	79580.0
32	97326.0
33	125234.0
34	196475.0
35	275547.0
36	214024.0
37	273247.0
38	397347.0
39	682268.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.055781720778242	23.168353617434597	27.037813527805937	18.73805113398122
2	36.028880738684514	24.47414374200534	27.037424299521778	12.459551219788366
3	26.337238000711228	24.020232793898316	36.5386990221524	13.10383018323806
4	22.8227189011024	26.837891727306662	36.2484762597285	14.090913111862442
5	20.963835384743312	30.49469145696993	35.45409671615173	13.087376442135024
6	18.87007029108968	39.31035824925117	32.19310676708756	9.626464692571577
7	83.47464089267737	3.3822876361635537	11.819942995748566	1.323128475410503
8	84.15487039582747	2.656518423766987	11.443877704473119	1.7447334759324231
9	78.87647486558355	4.674419386399302	13.670829640087684	2.778276107929465
10	38.8872317199821	19.70814955566053	25.484615752068663	15.920002972288716
11	33.18125122739601	25.781066167039086	24.737226677706598	16.300455927858305
12	26.286744476939113	22.32825744266402	32.65512074038296	18.729877340013907
13	22.00576411704448	27.60343299346627	33.68339109834914	16.707411791140103
14	20.504652162605424	28.87242335298936	34.187229419996775	16.435695064408435
15	20.047273544330448	23.912381174797115	39.96295254422606	16.077392736646374
16	20.812567119763774	21.224547566350154	38.86861953112145	19.09426578276462
17	21.789600881778988	22.967724129145946	32.798427517732	22.44424747134307
18	23.794409620307807	22.90502299100706	34.64793417088183	18.652633217803302
19	25.54204461617668	25.84801343191424	30.220214747859686	18.38972720404939
20	25.791186102427194	24.328572363730295	30.922948722712	18.957292811130515
21	23.61097694530104	25.90144386001228	32.14852243635675	18.339056758329928
22	23.040332896336125	23.551035789540727	32.1640561833336	21.24457513078955
23	21.41442020024026	26.40903292694362	31.095730696488634	21.08081617632749
24	21.722052082282858	24.562781637624926	34.7706118491705	18.94455443092171
25	20.969779962174087	25.13236415490578	33.776239736315524	20.1216161466046
26	20.0491489169723	27.52441965178222	32.20980819891688	20.216623232328594
27	20.9202772013071	25.893376219213373	32.992581662747845	20.193764916731684
28	20.034782854847926	25.519540144474462	35.349217385765925	19.09645961491169
29	20.532110448833286	24.697490008333023	34.52277958533034	20.24761995750335
30	21.237321330948426	25.45082366012586	33.87903138772268	19.43282362120303
31	23.821443293862046	24.69051928360765	31.467691398585686	20.020346023944615
32	24.151862722722615	24.72229446535072	31.251952775993814	19.87389003593285
33	24.152287335396245	25.2759186229811	29.683539712784913	20.888254328837746
34	21.849718959486644	26.466744866167392	30.357576947777947	21.325959226568013
35	21.957853653703594	26.72628936292176	29.89683681250343	21.419020170871217
36	25.38412408597699	26.25270469427003	27.128468333625257	21.234702886127728
37	22.314174455655397	28.08915450770584	28.866195700442837	20.73047533619593
38	23.986016089281893	28.086889906779827	26.807673458700226	21.119420545238057
39	22.819711227997544	27.490556791060488	27.400680441809488	22.289051539132483
40	23.729196190516628	26.14980688969447	27.169054228346077	22.951942691442817
41	21.30649781236012	25.098501294184043	28.204224542258693	25.390776351197143
42	22.812315890598544	26.465011031083417	27.39958352573595	23.32308955258209
43	22.459038146141065	26.30266745220011	28.5828375095759	22.65545689208292
44	22.11319112347206	27.79005026352524	27.99082128937177	22.105937323630933
45	21.27833183834288	28.653323213397947	27.05978723366612	23.008557714593053
46	23.08838489723489	27.74709361471	27.36009454708866	21.804426940966454
47	22.828026559522733	26.539672092862794	28.727170434219534	21.905130913394938
48	23.42386429378951	25.857850292186598	28.573319108808764	22.144966305215128
49	23.060431229554457	25.158937831396923	28.583049815912716	23.197581123135908
50	21.601674389309668	27.389003593284748	28.32771606150515	22.681605955900437
51	20.806021007712026	28.296153186098888	27.445158619371888	23.45266718681719
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1284.0
1	1726.0
2	2168.0
3	20092.0
4	38016.0
5	28733.5
6	19451.0
7	20310.0
8	21169.0
9	22832.5
10	24496.0
11	25796.0
12	27096.0
13	27549.0
14	28002.0
15	27838.0
16	27674.0
17	27291.5
18	26909.0
19	26102.5
20	25296.0
21	24566.0
22	23836.0
23	23937.5
24	24039.0
25	23763.5
26	24709.5
27	25931.0
28	28994.0
29	32057.0
30	35752.0
31	39447.0
32	44422.0
33	49397.0
34	58443.0
35	67489.0
36	74588.5
37	81688.0
38	91534.0
39	101380.0
40	120868.5
41	140357.0
42	157507.0
43	174657.0
44	182591.0
45	190525.0
46	196782.5
47	203040.0
48	210411.5
49	217783.0
50	221156.0
51	224529.0
52	212831.5
53	201134.0
54	185881.5
55	170629.0
56	156449.0
57	142269.0
58	129516.5
59	116764.0
60	105837.0
61	94910.0
62	85865.5
63	76821.0
64	65512.0
65	54203.0
66	46882.0
67	39561.0
68	32224.5
69	24888.0
70	20282.5
71	15677.0
72	13756.0
73	11835.0
74	9713.0
75	5935.5
76	4280.0
77	3336.5
78	2393.0
79	1713.0
80	1033.0
81	779.0
82	525.0
83	383.0
84	241.0
85	168.5
86	96.0
87	65.5
88	35.0
89	20.0
90	5.0
91	6.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2826105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.531224376712494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24976579925878	22.325616254438557
2	6.194218394256102	3.5345726968976123
3	2.568170523599191	2.1981914833940315
4	1.6235673050306845	1.852894522820995
5	1.200444606832201	1.712507721467197
6	0.9337486949989041	1.5984596117085728
7	0.7692650222509541	1.536365106849909
8	0.6482455847680804	1.479619218418504
9	0.5822194674940905	1.4950290837207565
>10	6.161530044565564	38.60773485665035
>50	0.9594669746498393	18.459176925643455
>100	0.10759350776677681	4.062742717380749
>500	0.0013860585583453694	0.24888781465171778
>1k	2.5201064697188394E-4	0.12683463962028832
>5k	0.0	0.0
>10k+	1.2600532348594197E-4	0.7613673463374337
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21178	0.7493706001723219	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.076877893779602E-5	0.0	0.0	0.02894443058555857	0.0
2	7.076877893779602E-5	0.0	0.0	0.08456869083066623	0.0
3	7.076877893779602E-5	0.0	0.0	0.15218825910573033	0.0
4	7.076877893779602E-5	0.0	0.0	0.2412153830094777	0.0
5	7.076877893779602E-5	0.0	0.0	0.3814791028641894	0.0
6	7.076877893779602E-5	0.0	0.0	0.8343992880660839	0.0
7	7.076877893779602E-5	0.0	0.0	1.0820546299589011	0.0
8	7.076877893779602E-5	0.0	0.0	1.7295889572397345	0.0
9	7.076877893779602E-5	0.0	0.0	2.153423174298195	0.0
10	1.0615316840669402E-4	0.0	0.0	2.714973435169606	0.0
11	1.0615316840669402E-4	0.0	0.0	3.061351223680649	0.0
12	1.0615316840669402E-4	0.0	0.0	3.380730723026922	0.0
13	1.0615316840669402E-4	0.0	0.0	3.597637030471267	0.0
14	1.0615316840669402E-4	0.0	0.0	3.715183972286946	0.0
15	1.0615316840669402E-4	0.0	0.0	3.797877290475761	0.0
16	1.0615316840669402E-4	0.0	0.0	3.910470417765794	0.0
17	1.0615316840669402E-4	0.0	0.0	4.032369639486148	0.0
18	1.0615316840669402E-4	0.0	0.0	4.207876211251882	0.0
19	1.4153755787559203E-4	0.0	0.0	4.3090401807434615	0.0
20	2.8307511575118407E-4	0.0	0.0	4.420607160738897	0.0
21	2.8307511575118407E-4	3.538438946889801E-5	0.0	4.551777092500101	0.0
22	3.538438946889801E-4	3.538438946889801E-5	0.0	4.690731589944464	0.0
23	3.538438946889801E-4	3.538438946889801E-5	0.0	4.858878208700667	0.0
24	3.538438946889801E-4	3.538438946889801E-5	0.0	4.9947542642612355	0.0
25	3.538438946889801E-4	1.4153755787559203E-4	0.0	5.118988855686537	0.0
26	3.538438946889801E-4	1.4153755787559203E-4	0.0	5.242551143711928	0.0
27	3.538438946889801E-4	1.4153755787559203E-4	0.0	5.381010259703727	0.0
28	3.538438946889801E-4	1.4153755787559203E-4	0.0	5.517240159158984	0.0
29	3.538438946889801E-4	1.4153755787559203E-4	0.0	5.66312999693925	0.0
30	3.538438946889801E-4	1.4153755787559203E-4	0.0	5.867510230511606	0.0
31	3.538438946889801E-4	1.4153755787559203E-4	0.0	6.020547714964589	0.0
32	3.538438946889801E-4	1.4153755787559203E-4	0.0	6.186111273289563	0.0
33	3.538438946889801E-4	1.4153755787559203E-4	0.0	6.349587152635872	0.0
34	3.538438946889801E-4	1.4153755787559203E-4	0.0	6.5140891792767786	0.0
35	3.538438946889801E-4	1.4153755787559203E-4	0.0	6.6975572386730144	0.0
36	3.538438946889801E-4	1.4153755787559203E-4	0.0	6.861528499471888	0.0
37	3.538438946889801E-4	1.4153755787559203E-4	0.0	7.050162679730583	0.0
38	3.538438946889801E-4	1.4153755787559203E-4	0.0	7.259921340502211	0.0
39	3.538438946889801E-4	1.4153755787559203E-4	0.0	7.546641048368691	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	20	7.0350355E-4	45.0	2
CGTTTTT	26360	0.0	43.685505	1
GTTTTTT	30170	0.0	38.72058	2
TCGTTAG	105	0.0	38.57143	1
TCGATAG	210	0.0	38.57143	1
AGGGCGA	3245	0.0	38.20493	6
CAGTACG	65	9.094947E-12	38.076927	1
TAACGGG	800	0.0	37.687496	3
GGGCGAT	6335	0.0	37.434887	7
GCGATAT	465	0.0	37.25806	9
TAGGGAC	1965	0.0	36.29771	5
GGCGATA	1460	0.0	36.215755	8
TACGGGT	430	0.0	36.104652	4
TAGGGTG	2500	0.0	36.09	5
ACGGGTA	505	0.0	36.08911	5
TATGGGC	1765	0.0	36.076485	4
ACGATAC	25	0.0021076282	35.999996	38
CGCGTAA	25	0.0021076282	35.999996	31
CGTTAGG	395	0.0	35.886078	2
AGTACGG	290	0.0	35.68966	2
>>END_MODULE