FastQCFastQC Report
Thu 26 May 2016
SRR1547662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547662_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4578797
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT981342.1432267034332377No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT59580.13012151445019293No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58290.127304180552228No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT47080.1028217673768896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT620650.044.119071
GTTTTTT695500.039.6815222
TTACGAG1650.039.5454521
AGGGCGA51650.038.5963176
CGTTAGG5350.038.2710272
TACGGGA10600.038.2075464
GTTACGG6050.037.1900832
ACGTGCG1350.036.6666641
TGTTACG3500.036.6428571
ACGGGAC9700.036.4175265
TGATTCG2350.036.3829815
ACGGGAA15100.036.3576135
GGGCGAT101550.036.3146257
GACCGAT26800.036.1847049
ACGGGAT10700.036.1682245
TGGGCGA27400.036.1313866
TCGCAAG751.8189894E-1236.0000041
TTACGGG15350.035.9120523
ACGGGTA6650.035.864665
TTAACGG5800.035.6896552
Produced by FastQC (version 0.11.5)