##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547662_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4578797
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.393385424162723	31.0	31.0	33.0	30.0	34.0
2	31.800728444611106	31.0	31.0	34.0	30.0	34.0
3	31.799768367106033	31.0	31.0	34.0	30.0	34.0
4	35.566765244233366	37.0	35.0	37.0	33.0	37.0
5	35.47962685395312	37.0	35.0	37.0	33.0	37.0
6	35.541620648393014	37.0	35.0	37.0	33.0	37.0
7	36.009560371425074	37.0	35.0	37.0	35.0	37.0
8	36.037046193574426	37.0	35.0	37.0	35.0	37.0
9	37.77351911430011	39.0	38.0	39.0	35.0	39.0
10	37.12076731071502	39.0	37.0	39.0	33.0	39.0
11	36.89513730353191	39.0	37.0	39.0	32.0	39.0
12	36.494660278671454	39.0	35.0	39.0	32.0	39.0
13	36.34158732086179	39.0	35.0	39.0	32.0	39.0
14	37.484884785239444	40.0	36.0	41.0	32.0	41.0
15	37.623858843272586	40.0	36.0	41.0	32.0	41.0
16	37.66980868555649	40.0	35.0	41.0	33.0	41.0
17	37.59459198562417	39.0	35.0	41.0	32.0	41.0
18	37.340484629478006	39.0	36.0	40.0	32.0	41.0
19	37.13113335227572	38.0	36.0	40.0	32.0	41.0
20	36.852875329480646	38.0	35.0	40.0	32.0	41.0
21	36.745870367260224	38.0	35.0	40.0	32.0	41.0
22	36.66403817421912	38.0	35.0	40.0	32.0	41.0
23	36.55474090683644	38.0	35.0	40.0	31.0	41.0
24	36.42686648916735	38.0	35.0	40.0	31.0	41.0
25	36.29768714358815	38.0	35.0	40.0	31.0	41.0
26	36.14761082441523	37.0	35.0	40.0	31.0	41.0
27	36.05263456755126	37.0	35.0	40.0	30.0	41.0
28	36.06487249816928	37.0	35.0	40.0	31.0	41.0
29	35.930900627391864	37.0	35.0	40.0	30.0	41.0
30	35.86760015785806	37.0	35.0	40.0	30.0	41.0
31	35.59078268811655	37.0	35.0	40.0	29.0	41.0
32	35.12100711169331	37.0	34.0	40.0	25.0	41.0
33	34.61891758905232	37.0	34.0	40.0	23.0	41.0
34	34.07505726940941	37.0	33.0	40.0	20.0	41.0
35	33.70819060115572	37.0	33.0	40.0	18.0	41.0
36	33.4489478349881	37.0	33.0	40.0	15.0	41.0
37	33.14934992750279	36.0	33.0	40.0	15.0	41.0
38	33.17663176594201	36.0	33.0	40.0	15.0	41.0
39	33.072085964937955	36.0	33.0	40.0	14.0	41.0
40	32.94943431648095	36.0	32.0	40.0	12.0	41.0
41	32.907887595803004	36.0	32.0	40.0	12.0	41.0
42	32.85314592457364	36.0	32.0	40.0	11.0	41.0
43	32.7837462547477	36.0	32.0	40.0	10.0	41.0
44	32.63005042590881	35.0	32.0	40.0	10.0	41.0
45	32.596724205069584	35.0	32.0	40.0	10.0	41.0
46	32.41511034448568	35.0	31.0	40.0	10.0	41.0
47	32.44164766422272	35.0	31.0	40.0	10.0	41.0
48	32.24174777785519	35.0	31.0	40.0	10.0	41.0
49	32.22282599556171	35.0	31.0	40.0	10.0	41.0
50	32.083624366836965	35.0	31.0	40.0	10.0	41.0
51	31.445394063113085	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	6.0
13	18.0
14	50.0
15	166.0
16	494.0
17	1377.0
18	3448.0
19	6520.0
20	11321.0
21	18063.0
22	27780.0
23	41418.0
24	64582.0
25	103548.0
26	143584.0
27	145926.0
28	125864.0
29	115401.0
30	121064.0
31	137718.0
32	165202.0
33	207055.0
34	320581.0
35	494737.0
36	373727.0
37	418332.0
38	565653.0
39	964554.0
40	605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.066957980447704	22.867119900707543	26.062238618571648	22.00368350027311
2	38.9301382000556	24.022903832600573	25.55149311052663	11.495464856817195
3	25.009800609199317	23.3807482620435	39.09684137558402	12.512609753173159
4	21.700831026140712	26.003598761858193	38.79521193012051	13.50035828188059
5	19.776875891200245	30.12850318544369	37.551042337102956	12.543578586253116
6	17.920842526978156	37.9645570659717	34.45481422303719	9.659786184012962
7	78.20783494005084	3.6717941415616373	16.419640355316037	1.7007305630714793
8	78.65710141768677	2.9362515962162115	15.988457230141453	2.4181897559555487
9	73.35175593065165	5.255354190194499	18.38179329636147	3.01109658279238
10	36.87422700766162	22.94941225828531	26.623019103052613	13.553341631000457
11	30.782626965117693	24.57385640813515	30.10546219891382	14.538054427833336
12	25.928273299733533	22.283189230708413	35.27765043962421	16.510887029933848
13	19.83935955230162	29.437623026310185	36.568862956798476	14.154154464589716
14	16.992629286688185	32.19577107262017	36.03097058026377	14.78062906042788
15	15.869102735936972	25.79767130973485	44.457834667053376	13.875391287274802
16	17.25994841002997	23.16842174920618	43.52361548240728	16.04801435835657
17	17.19320598838516	24.501086202336552	35.82650639458356	22.47920141469473
18	19.912828631625292	25.55712777832256	37.47790085474416	17.052142735307985
19	22.43145961701294	27.821696397547218	33.03963901435246	16.707204971087382
20	23.698975953727583	25.56256588793956	32.81783402933128	17.92062412900157
21	20.00272123878827	28.53245077255008	34.19926675063341	17.265561238028244
22	20.342614009749724	25.919799458241975	33.203459336589944	20.534127195418357
23	18.10451522528734	29.68539553074749	32.33491242350338	19.875176820461792
24	18.85964806913257	26.72750069505156	38.110315875545474	16.302535360270394
25	17.78449667019525	27.89791729137588	36.358764103322336	17.958821935106535
26	17.125852052405904	31.471366824080647	34.21033952804634	17.192441595467105
27	18.207620910033793	29.71883226096287	34.47759313199515	17.595953697008188
28	16.883910773943462	28.885381902713746	38.29208851145836	15.938618811884432
29	17.33073119424163	26.91047451983567	37.381652866462524	18.377141419460177
30	18.8482258549571	28.914690911171647	35.24864718833353	16.988436045537725
31	21.64710512390045	28.451578001820128	31.888550638956044	18.012766235323383
32	22.430891782273815	28.75242121456793	32.32956167307701	16.48712533008124
33	21.454587307539512	30.33506399169913	30.23573659194762	17.974612108813734
34	18.605804100946166	30.551649265079888	31.686772748387842	19.155773885586104
35	19.879697658577133	29.92997942472663	30.862473265357693	19.32784965133855
36	23.148307295562567	29.607274574522524	29.60229510065635	17.642123029258556
37	21.101110182434383	31.860595697952977	29.663337335112256	17.374956784500384
38	20.077609904959754	32.96092838359071	28.697690681635375	18.263771029814162
39	20.991780155355215	31.655978633689152	28.316673571682692	19.035567639272934
40	21.437530425568113	29.897525485405886	28.652700698458567	20.012243390567434
41	19.646863575738344	28.415957291838883	28.97014215742694	22.967036974995832
42	21.101656177375848	30.208349485683684	27.630357930259848	21.05963640668062
43	20.787774605425835	29.845983562931487	29.056190960201995	20.310050871440684
44	20.455547603442565	31.95638505048378	28.20090080429423	19.387166541779425
45	19.11436562922532	32.938062115442115	27.28017861460117	20.667393640731397
46	21.461313965218377	31.609962180022393	27.27214156906279	19.656582285696437
47	21.12816969173344	29.98807328649862	28.322679516038818	20.561077505729124
48	21.330013101694615	28.38828626820538	29.31049356413923	20.971207065960776
49	20.4562683167653	27.564292542342457	29.819098772013696	22.16034036887855
50	19.18431850112595	30.654165275289557	29.438037982465698	20.72347824111879
51	18.647518114474174	31.586440717944036	27.96077222903745	21.80526893854434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4147.0
1	4710.0
2	5273.0
3	68003.5
4	130734.0
5	87511.0
6	44288.0
7	47781.5
8	51275.0
9	58410.0
10	65545.0
11	71810.5
12	78076.0
13	81197.0
14	84318.0
15	82511.0
16	80704.0
17	76459.5
18	72215.0
19	67695.5
20	63176.0
21	60175.0
22	57174.0
23	55098.0
24	53022.0
25	52985.0
26	51716.5
27	50485.0
28	53022.5
29	55560.0
30	62536.0
31	69512.0
32	78295.5
33	87079.0
34	96520.5
35	105962.0
36	118851.0
37	131740.0
38	150127.0
39	168514.0
40	208719.0
41	248924.0
42	293973.5
43	339023.0
44	370749.5
45	402476.0
46	403888.5
47	405301.0
48	378772.5
49	352244.0
50	320229.0
51	288214.0
52	260637.5
53	233061.0
54	215386.5
55	197712.0
56	177566.0
57	157420.0
58	142117.5
59	126815.0
60	110042.0
61	93269.0
62	82215.0
63	71161.0
64	62009.0
65	52857.0
66	42905.5
67	32954.0
68	28414.5
69	23875.0
70	19600.0
71	15325.0
72	12981.5
73	10638.0
74	8581.0
75	5507.0
76	4490.0
77	3412.0
78	2334.0
79	1882.5
80	1431.0
81	1021.0
82	611.0
83	431.5
84	252.0
85	162.0
86	72.0
87	55.5
88	39.0
89	27.5
90	16.0
91	11.5
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4578797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.934961476802666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95177376098029	22.334497200150267
2	6.881557284299141	3.8447207527461456
3	2.85728892150344	2.394547678508809
4	1.6447999541254528	1.8378969342216531
5	1.1667528549467403	1.629659802794336
6	0.8706061378101088	1.4592209352716006
7	0.6451882964545205	1.2616317144718678
8	0.4940986107842928	1.1042100526400747
9	0.39575567164490966	0.9949877497464044
>10	3.828691261046942	23.422260904613694
>50	0.7706969658979294	15.151907995744624
>100	0.4880033992048024	20.49986428513267
>500	0.002712566071531342	0.5195742850933087
>1k	0.0018349711649702316	1.095222386934829
>5k	1.5956270999741145E-4	0.2626955816776043
>10k+	7.978135499870573E-5	2.187101740251974
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98134	2.1432267034332377	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	5958	0.13012151445019293	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5829	0.127304180552228	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	4708	0.1028217673768896	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.183979765864265E-5	0.0	0.0	0.07482314677850972	0.0
2	2.183979765864265E-5	0.0	0.0	0.23316167980366895	0.0
3	2.183979765864265E-5	0.0	0.0	0.4223598469204903	0.0
4	2.183979765864265E-5	0.0	0.0	0.6341403648163481	0.0
5	2.183979765864265E-5	0.0	0.0	1.038482378668458	0.0
6	2.183979765864265E-5	0.0	0.0	2.003517517810901	0.0
7	2.183979765864265E-5	0.0	0.0	2.5515654002568797	0.0
8	2.183979765864265E-5	0.0	0.0	3.6638226154162328	0.0
9	2.183979765864265E-5	0.0	0.0	4.3022654203713335	0.0
10	2.183979765864265E-5	0.0	0.0	5.1202532018781355	0.0
11	2.183979765864265E-5	0.0	0.0	5.610316421540418	0.0
12	2.183979765864265E-5	0.0	0.0	6.049558432051039	0.0
13	2.183979765864265E-5	0.0	0.0	6.327972172603415	0.0
14	2.183979765864265E-5	0.0	0.0	6.478033422315949	0.0
15	2.183979765864265E-5	0.0	0.0	6.60079492495518	0.0
16	2.183979765864265E-5	0.0	0.0	6.789665495107121	0.0
17	2.183979765864265E-5	0.0	0.0	6.991749142842542	0.0
18	2.183979765864265E-5	0.0	0.0	7.321202490523166	0.0
19	2.183979765864265E-5	0.0	0.0	7.46514859689128	0.0
20	6.551939297592796E-5	0.0	0.0	7.639561220993199	0.0
21	6.551939297592796E-5	0.0	0.0	7.826225971581619	0.0
22	1.0919898829321326E-4	0.0	0.0	8.0231554270696	0.0
23	1.0919898829321326E-4	0.0	0.0	8.258413727448499	0.0
24	1.0919898829321326E-4	0.0	0.0	8.446214147515166	0.0
25	1.0919898829321326E-4	0.0	0.0	8.614402429284373	0.0
26	1.0919898829321326E-4	0.0	0.0	8.771932889796163	0.0
27	1.310387859518559E-4	0.0	0.0	8.942348830926552	0.0
28	1.310387859518559E-4	0.0	0.0	9.116499377456568	0.0
29	1.310387859518559E-4	0.0	0.0	9.297114504093543	0.0
30	1.310387859518559E-4	0.0	0.0	9.560917420012288	0.0
31	1.310387859518559E-4	0.0	0.0	9.74911095643681	0.0
32	1.310387859518559E-4	0.0	0.0	9.938767759304463	0.0
33	1.310387859518559E-4	0.0	0.0	10.125934825239032	0.0
34	1.310387859518559E-4	0.0	0.0	10.323672353240381	0.0
35	1.310387859518559E-4	0.0	0.0	10.5338367261095	0.0
36	1.310387859518559E-4	0.0	0.0	10.736488208584046	0.0
37	1.310387859518559E-4	0.0	0.0	10.946128426309356	0.0
38	1.310387859518559E-4	0.0	0.0	11.162538981308845	0.0
39	1.310387859518559E-4	0.0	0.0	11.373620625679628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	62065	0.0	44.11907	1
GTTTTTT	69550	0.0	39.681522	2
TTACGAG	165	0.0	39.545452	1
AGGGCGA	5165	0.0	38.596317	6
CGTTAGG	535	0.0	38.271027	2
TACGGGA	1060	0.0	38.207546	4
GTTACGG	605	0.0	37.190083	2
ACGTGCG	135	0.0	36.666664	1
TGTTACG	350	0.0	36.642857	1
ACGGGAC	970	0.0	36.417526	5
TGATTCG	235	0.0	36.38298	15
ACGGGAA	1510	0.0	36.357613	5
GGGCGAT	10155	0.0	36.314625	7
GACCGAT	2680	0.0	36.184704	9
ACGGGAT	1070	0.0	36.168224	5
TGGGCGA	2740	0.0	36.131386	6
TCGCAAG	75	1.8189894E-12	36.000004	1
TTACGGG	1535	0.0	35.912052	3
ACGGGTA	665	0.0	35.86466	5
TTAACGG	580	0.0	35.689655	2
>>END_MODULE