FastQCFastQC Report
Thu 26 May 2016
SRR1547661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547661_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences688026
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28820.4188795190879414No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9130.13269847360419518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG302.1642263E-645.0000041
TATCTCG253.8891667E-545.0000041
GCGTATG253.8891667E-545.0000041
AATACCG253.8891667E-545.0000041
TGCGTAG253.8891667E-545.0000041
CGGGTAT453.8380676E-1045.06
CGTTCGA351.210974E-745.014
ACGGGTA351.210974E-745.05
TAGTCGG351.210974E-745.02
CATACGA1200.043.12500418
ACATACG1250.041.39999817
CGTATGG603.6379788E-1241.2500042
AGCAACG1700.041.0294110
TACGGGA1600.040.781254
CGGCTAC1350.040.00000425
AAGTACG451.9263098E-840.01
TCGTAAG451.9263098E-840.01
GCGATCT850.039.7058839
GTTCGAA403.455516E-739.37515
GGCGATA3150.039.2857138
Produced by FastQC (version 0.11.5)