##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547661_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	688026
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.384495644059964	31.0	31.0	33.0	30.0	34.0
2	31.78975794519393	31.0	31.0	34.0	30.0	34.0
3	31.925139456939128	31.0	31.0	34.0	30.0	34.0
4	35.625517058948354	37.0	35.0	37.0	33.0	37.0
5	35.50793720004767	37.0	35.0	37.0	33.0	37.0
6	35.56058637318939	37.0	35.0	37.0	33.0	37.0
7	35.963749916427574	37.0	35.0	37.0	35.0	37.0
8	35.9681843418708	37.0	35.0	37.0	35.0	37.0
9	37.69119044919814	39.0	37.0	39.0	35.0	39.0
10	37.068625313578266	39.0	37.0	39.0	33.0	39.0
11	36.79798292506388	39.0	35.0	39.0	32.0	39.0
12	36.1827183856424	38.0	35.0	39.0	32.0	39.0
13	35.9157226616436	38.0	35.0	39.0	31.0	39.0
14	36.98494388293466	39.0	35.0	41.0	31.0	41.0
15	37.183182030911624	39.0	35.0	41.0	32.0	41.0
16	37.27293445305846	39.0	35.0	41.0	32.0	41.0
17	37.18868909023787	39.0	35.0	41.0	32.0	41.0
18	37.10477365680948	39.0	35.0	41.0	32.0	41.0
19	37.055964454831646	38.0	35.0	41.0	32.0	41.0
20	36.948609500222375	38.0	35.0	41.0	32.0	41.0
21	36.80856537398296	38.0	35.0	41.0	31.0	41.0
22	36.6883475333781	38.0	35.0	41.0	31.0	41.0
23	36.59115207855517	38.0	35.0	40.0	31.0	41.0
24	36.45819780066451	38.0	35.0	40.0	31.0	41.0
25	36.32813004159726	38.0	35.0	40.0	31.0	41.0
26	36.130537218070245	38.0	35.0	40.0	30.0	41.0
27	36.108529328833505	38.0	35.0	40.0	30.0	41.0
28	36.133371413289616	38.0	35.0	40.0	30.0	41.0
29	36.13657478060422	38.0	35.0	40.0	30.0	41.0
30	36.168359916631054	38.0	35.0	40.0	30.0	41.0
31	36.04827288503632	38.0	35.0	40.0	30.0	41.0
32	35.83647274957632	38.0	35.0	40.0	30.0	41.0
33	35.736546293308685	38.0	35.0	40.0	29.0	41.0
34	35.5662954597646	38.0	35.0	40.0	29.0	41.0
35	35.449233023170635	38.0	34.0	40.0	28.0	41.0
36	35.285887451927685	37.0	34.0	40.0	27.0	41.0
37	35.01447619712046	37.0	34.0	40.0	26.0	41.0
38	35.08417850488208	37.0	34.0	40.0	27.0	41.0
39	34.99044367509367	37.0	34.0	40.0	26.0	41.0
40	34.825614148302535	37.0	34.0	40.0	25.0	41.0
41	34.776508445901754	37.0	34.0	40.0	25.0	41.0
42	34.74327132986254	36.0	34.0	40.0	25.0	41.0
43	34.6394729268952	36.0	34.0	40.0	25.0	41.0
44	34.48581012926837	36.0	34.0	40.0	24.0	41.0
45	34.41467182926227	36.0	33.0	40.0	24.0	41.0
46	34.21785949949566	35.0	33.0	40.0	23.0	41.0
47	34.21996261769177	35.0	33.0	40.0	24.0	41.0
48	34.05110998712258	35.0	33.0	40.0	23.0	41.0
49	34.03838081700401	35.0	33.0	40.0	24.0	41.0
50	33.87315159601527	35.0	33.0	39.0	24.0	41.0
51	33.165162363050236	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	8.0
15	30.0
16	80.0
17	179.0
18	367.0
19	712.0
20	1226.0
21	1882.0
22	2842.0
23	4149.0
24	5982.0
25	7878.0
26	10126.0
27	12184.0
28	13512.0
29	15321.0
30	17951.0
31	21368.0
32	26736.0
33	34498.0
34	57214.0
35	89124.0
36	54334.0
37	66763.0
38	93574.0
39	149866.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.13562859543099	24.28323929618939	28.143849215000593	13.43728289337903
2	31.46857822233462	26.202207474717525	28.462005796292583	13.86720850665527
3	29.359646292436626	26.14305273347228	30.498702083932876	13.99859889015822
4	25.69321508198818	29.066343423068314	30.02110385363344	15.219337641310066
5	23.424114786359816	33.187263272027515	28.428722170383097	14.959899771229576
6	21.79699604375416	41.74260856421124	25.5782484964231	10.882146895611502
7	87.52808760134064	4.519305956460948	6.181597788455669	1.7710086537427365
8	87.98955272039139	3.701458956492923	5.739172647545296	2.5698156755704
9	82.11114114873565	5.941054553170956	8.487905980297256	3.4598983177961298
10	47.51666361445643	23.766979736230898	14.749587951618107	13.966768697694565
11	39.68890710525474	24.547473496641114	19.961745631705778	15.801873766398364
12	34.67848598744815	22.11369919160032	25.41037693342984	17.797437887521692
13	22.241019961454946	33.87633606869508	26.621523023839217	17.26112094601076
14	18.79492926139419	35.276137820373066	27.851418405699786	18.077514512532957
15	18.313261417446434	23.730643900085173	40.2913552685509	17.664739413917495
16	19.812623360163713	20.370451116672918	39.462462174394574	20.354463348768796
17	19.70492394182778	22.078671445555837	28.261868010801916	29.954536601814468
18	24.467970687154263	23.216564490295426	30.413094853973544	21.902369968576767
19	28.510841160072438	26.0740146447954	24.412885559557342	21.00225863557482
20	30.787353966274527	23.33981564650173	23.894009819396363	21.978820567827377
21	25.21416341824291	27.57541139433684	25.288724554013946	21.921700633406296
22	25.14643341966728	24.464482446884276	25.110678956899886	25.278405176548564
23	23.600997636717217	28.75719812914047	24.15504646626726	23.48675776787505
24	23.12441680982986	24.886704862897624	32.260554106966886	19.72832422030563
25	21.668803213832035	25.77824093856918	29.833610939121485	22.719344908477296
26	21.066645737225045	30.86729280579513	26.11238528776529	21.953676169214535
27	22.418920215224425	29.140468528805595	26.78081932950208	21.659791926467893
28	20.37423004363207	28.138180824561864	32.01143561435178	19.476153517454282
29	20.759680593465944	25.62810126361504	30.806539287759477	22.805678855159545
30	22.62923203483589	27.392133436817794	28.508515666559113	21.4701188617872
31	27.815082569554058	26.434756826050176	23.521640170574948	22.228520433820815
32	28.537148305441946	26.716141541162692	24.870717094993502	19.875993058401864
33	26.33141770805173	27.41001066820149	23.96639080499865	22.29218081874813
34	22.272414123884854	28.369712772482437	26.766139651699213	22.5917334519335
35	22.587809181629765	26.855380465273115	28.29587835343432	22.2609319996628
36	28.353288974544565	26.47690639597922	25.054867112580048	20.114937516896163
37	23.205082366073377	29.790007935746615	27.116707798833183	19.88820189934683
38	22.98154430210486	29.587980686776373	25.10733605997448	22.32313895114429
39	23.415975559063178	28.575809635101002	26.296825992041	21.711388813794827
40	24.885978146174708	26.04959696290547	25.91239284561921	23.152032045300615
41	19.713644542502752	25.450201009845557	27.285596765238523	27.550557682413164
42	23.89851546307843	26.4966730908425	24.58003040582769	25.024781040251387
43	23.94749617020287	25.761962483975896	26.83343362024109	23.457107725580137
44	23.123690093106948	28.891059349501326	26.09363599631409	21.89161456107763
45	21.383784915104954	30.460476784307573	24.102577518872835	24.053160781714645
46	23.96639080499865	28.887425765886753	25.336978544415473	21.809204884699124
47	23.979762392700277	26.468040451959663	26.671230447686572	22.880966707653492
48	24.86010703083895	23.867121300648524	27.9932735100127	23.279498158499823
49	22.848409798466918	24.78874344864874	28.103007735172795	24.259839017711542
50	21.28466075409941	29.030007586922586	27.289957065576008	22.395374593401993
51	21.362564786795847	29.782014051794555	24.438029958170183	24.417391203239415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	522.0
1	659.5
2	797.0
3	2829.0
4	4861.0
5	3568.5
6	2276.0
7	2403.0
8	2530.0
9	2735.5
10	2941.0
11	3171.5
12	3402.0
13	3344.5
14	3287.0
15	3274.0
16	3261.0
17	3157.0
18	3053.0
19	3099.0
20	3145.0
21	3224.0
22	3303.0
23	3328.0
24	3353.0
25	3428.5
26	4047.0
27	4590.0
28	5600.5
29	6611.0
30	7660.0
31	8709.0
32	9982.0
33	11255.0
34	12358.5
35	13462.0
36	15216.5
37	16971.0
38	19561.5
39	22152.0
40	28881.5
41	35611.0
42	43610.0
43	51609.0
44	55878.5
45	60148.0
46	58535.5
47	56923.0
48	53680.5
49	50438.0
50	49575.0
51	48712.0
52	46160.0
53	43608.0
54	40843.5
55	38079.0
56	36155.0
57	34231.0
58	33373.0
59	32515.0
60	30309.5
61	28104.0
62	25650.0
63	23196.0
64	20320.5
65	17445.0
66	14938.5
67	12432.0
68	11137.5
69	9843.0
70	8743.5
71	7644.0
72	6318.0
73	4992.0
74	4287.0
75	2964.0
76	2346.0
77	1844.5
78	1343.0
79	968.5
80	594.0
81	486.5
82	379.0
83	250.0
84	121.0
85	93.5
86	66.0
87	58.5
88	51.0
89	37.0
90	23.0
91	12.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	688026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.826732756276133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4608144144801	20.998279162826876
2	5.964540884083357	3.3194737039054116
3	2.579562372625146	2.1534237831355663
4	1.7839262408764192	1.985633550471056
5	1.3223375415445817	1.8398166691076134
6	1.0621991933770356	1.7734519852820911
7	0.9018257999078745	1.7566405868725967
8	0.7976781886250968	1.7757422224304795
9	0.7107648678693212	1.7800437627674568
>10	9.059300801650405	55.1300526240872
>50	0.33184618708109553	5.702261624511232
>100	0.024153361717919136	1.2306902827079584
>500	5.25073080824329E-4	0.13339905355720835
>1k	5.25073080824329E-4	0.4210909883372119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2882	0.4188795190879414	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	913	0.13269847360419518	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028051265504501283	0.0
2	0.0	0.0	0.0	0.10726338830218625	0.0
3	0.0	0.0	0.0	0.17659216366823347	0.0
4	0.0	0.0	0.0	0.33501640926360343	0.0
5	0.0	0.0	0.0	0.5739608677579044	0.0
6	0.0	0.0	0.0	1.1703046105815769	0.0
7	0.0	0.0	0.0	1.4483464287686802	0.0
8	0.0	0.0	0.0	2.223171798740164	0.0
9	0.0	0.0	0.0	2.624900803167322	0.0
10	0.0	0.0	0.0	3.1895596968719206	0.0
11	0.0	0.0	0.0	3.5728300965370496	0.0
12	0.0	0.0	0.0	3.902759488740251	0.0
13	0.0	0.0	0.0	4.076735472205992	0.0
14	0.0	0.0	0.0	4.156674311726592	0.0
15	0.0	0.0	0.0	4.235014374456779	0.0
16	0.0	0.0	0.0	4.3707650582972155	0.0
17	0.0	0.0	0.0	4.501283381732667	0.0
18	0.0	0.0	0.0	4.71232191806705	0.0
19	0.0	0.0	0.0	4.804469598532614	0.0
20	1.453433445829082E-4	0.0	0.0	4.904029789571906	0.0
21	1.453433445829082E-4	0.0	0.0	5.026844915744463	0.0
22	1.453433445829082E-4	0.0	0.0	5.172914977050286	0.0
23	1.453433445829082E-4	0.0	0.0	5.3202931284573545	0.0
24	1.453433445829082E-4	0.0	0.0	5.453136945406133	0.0
25	1.453433445829082E-4	0.0	0.0	5.565487350768721	0.0
26	1.453433445829082E-4	0.0	0.0	5.672460052381742	0.0
27	1.453433445829082E-4	0.0	0.0	5.769113376529376	0.0
28	1.453433445829082E-4	0.0	0.0	5.8808824085136315	0.0
29	1.453433445829082E-4	0.0	0.0	5.999046547659536	0.0
30	1.453433445829082E-4	0.0	0.0	6.15369186629575	0.0
31	1.453433445829082E-4	0.0	0.0	6.2831927863191215	0.0
32	1.453433445829082E-4	0.0	0.0	6.425774607354954	0.0
33	1.453433445829082E-4	0.0	0.0	6.553240720554165	0.0
34	1.453433445829082E-4	0.0	0.0	6.675038443314643	0.0
35	1.453433445829082E-4	0.0	0.0	6.8168935476275605	0.0
36	1.453433445829082E-4	0.0	0.0	6.941743480624279	0.0
37	1.453433445829082E-4	0.0	0.0	7.0750233276068055	0.0
38	1.453433445829082E-4	0.0	0.0	7.227924526108025	0.0
39	1.453433445829082E-4	0.0	0.0	7.364692613360542	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.1642263E-6	45.000004	1
TATCTCG	25	3.8891667E-5	45.000004	1
GCGTATG	25	3.8891667E-5	45.000004	1
AATACCG	25	3.8891667E-5	45.000004	1
TGCGTAG	25	3.8891667E-5	45.000004	1
CGGGTAT	45	3.8380676E-10	45.0	6
CGTTCGA	35	1.210974E-7	45.0	14
ACGGGTA	35	1.210974E-7	45.0	5
TAGTCGG	35	1.210974E-7	45.0	2
CATACGA	120	0.0	43.125004	18
ACATACG	125	0.0	41.399998	17
CGTATGG	60	3.6379788E-12	41.250004	2
AGCAACG	170	0.0	41.02941	10
TACGGGA	160	0.0	40.78125	4
CGGCTAC	135	0.0	40.000004	25
AAGTACG	45	1.9263098E-8	40.0	1
TCGTAAG	45	1.9263098E-8	40.0	1
GCGATCT	85	0.0	39.705883	9
GTTCGAA	40	3.455516E-7	39.375	15
GGCGATA	315	0.0	39.285713	8
>>END_MODULE