FastQCFastQC Report
Thu 26 May 2016
SRR1547660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547660_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1068825
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43400.40605337637124883No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21950.20536570533062007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG600.045.0000041
ACACGAA253.890621E-545.00000442
GTCGACA207.0330634E-445.00000440
AAACGCG207.0330634E-445.0000041
TACTACG207.0330634E-445.0000041
ATCGGTA207.0330634E-445.00000435
ATCGGGT207.0330634E-445.0000044
CAGTACG406.8139343E-945.0000041
TAATGCG502.1827873E-1145.0000041
TACACCG207.0330634E-445.0000041
CGCTTAA207.0330634E-445.00000437
GCGTAAG850.042.352941
GCGATCG750.042.09
AAGGGTA5700.041.8421065
CGTTCGG650.041.5384645
TAGAACG556.184564E-1140.9090921
CGAATAT556.184564E-1140.90909214
TGCATAG950.040.2631571
ACGTAAG451.9281288E-840.01
CGTAAGG2100.039.6428572