FastQCFastQC Report
Thu 26 May 2016
SRR1547659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547659_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2899309
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175840.6064893393563777No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52910.18249175924332314No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA253.8922815E-545.036
CGTTTTT99500.041.8115581
ACGTATG700.041.7857131
TACGAAT2800.040.1785712
TACGGGA7700.040.0324674
GCGTAAT451.9301297E-840.023
CGTAAGG3950.039.30382
GTACGGG10700.039.112153
CGTTAGG4550.039.0659332
GCGATCG1450.038.7931069
GCTACGA2900.038.79310610
TAGTACG1750.038.5714261
AGGGCGA35000.038.5071456
GGGCGAT71600.038.0237437
TAAACGG1900.037.8947372
AGTACGG4150.037.4096372
GCGTTAG2650.037.358491
GCGAGAC2050.037.31707421
ATACGGG9350.036.577543
TAGGGAC22500.036.45
Produced by FastQC (version 0.11.5)