##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547659_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2899309
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40509652472365	31.0	31.0	33.0	30.0	34.0
2	31.811610973511275	31.0	31.0	34.0	30.0	34.0
3	31.94491411574275	31.0	31.0	34.0	30.0	34.0
4	35.638798072230315	37.0	35.0	37.0	33.0	37.0
5	35.52028121183358	37.0	35.0	37.0	33.0	37.0
6	35.5710343395616	37.0	35.0	37.0	33.0	37.0
7	35.986252931301905	37.0	35.0	37.0	35.0	37.0
8	36.004077868209286	37.0	35.0	37.0	35.0	37.0
9	37.746504080799944	39.0	37.0	39.0	35.0	39.0
10	37.100153174428804	39.0	37.0	39.0	33.0	39.0
11	36.8321596628714	39.0	37.0	39.0	32.0	39.0
12	36.23878413787561	38.0	35.0	39.0	32.0	39.0
13	36.00246748449372	38.0	35.0	39.0	31.0	39.0
14	37.060454059915656	39.0	35.0	41.0	32.0	41.0
15	37.250130979485114	39.0	35.0	41.0	32.0	41.0
16	37.34692473275529	39.0	35.0	41.0	32.0	41.0
17	37.28656448829704	39.0	35.0	41.0	32.0	41.0
18	37.192570712538746	39.0	35.0	41.0	32.0	41.0
19	37.15002091877754	39.0	35.0	41.0	32.0	41.0
20	37.027519315809386	39.0	35.0	41.0	32.0	41.0
21	36.92597546518843	38.0	35.0	41.0	32.0	41.0
22	36.81691361631341	38.0	35.0	41.0	31.0	41.0
23	36.70597649301954	38.0	35.0	41.0	31.0	41.0
24	36.59869334382779	38.0	35.0	40.0	31.0	41.0
25	36.46919179707992	38.0	35.0	40.0	31.0	41.0
26	36.29980281508456	38.0	35.0	40.0	31.0	41.0
27	36.25679360150988	38.0	35.0	40.0	30.0	41.0
28	36.3035685399521	38.0	35.0	40.0	30.0	41.0
29	36.29799721243924	38.0	35.0	40.0	30.0	41.0
30	36.31799680544571	38.0	35.0	40.0	30.0	41.0
31	36.182190308104445	38.0	35.0	40.0	30.0	41.0
32	35.972523453002076	38.0	35.0	40.0	30.0	41.0
33	35.8780702574303	38.0	35.0	40.0	30.0	41.0
34	35.68530708523996	38.0	35.0	40.0	29.0	41.0
35	35.589779495735016	38.0	35.0	40.0	29.0	41.0
36	35.42987380786249	38.0	35.0	40.0	27.0	41.0
37	35.17669865474843	38.0	34.0	40.0	26.0	41.0
38	35.27954384993114	38.0	34.0	40.0	27.0	41.0
39	35.19587529304396	37.0	34.0	40.0	27.0	41.0
40	35.06867601901005	37.0	34.0	40.0	26.0	41.0
41	35.03966151934823	37.0	34.0	40.0	26.0	41.0
42	34.993076281279436	37.0	34.0	40.0	26.0	41.0
43	34.92471550979906	37.0	34.0	40.0	26.0	41.0
44	34.75448494796519	37.0	34.0	40.0	25.0	41.0
45	34.71965009593665	37.0	34.0	40.0	25.0	41.0
46	34.530086306771715	36.0	34.0	40.0	24.0	41.0
47	34.559264983484	36.0	34.0	40.0	25.0	41.0
48	34.395632200638154	36.0	34.0	40.0	24.0	41.0
49	34.38397080131852	36.0	34.0	40.0	24.0	41.0
50	34.23918561284775	36.0	33.0	40.0	24.0	41.0
51	33.51933133032733	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	5.0
12	5.0
13	13.0
14	24.0
15	80.0
16	249.0
17	649.0
18	1358.0
19	2803.0
20	4759.0
21	7402.0
22	10970.0
23	16034.0
24	23125.0
25	31775.0
26	41763.0
27	48803.0
28	54201.0
29	62255.0
30	73197.0
31	88282.0
32	109617.0
33	140462.0
34	233344.0
35	370318.0
36	219100.0
37	269302.0
38	392600.0
39	696343.0
40	468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70993191826052	24.96888051601261	28.372415634208014	13.948771931518856
2	31.13459103531221	26.356245574376512	28.788480289613837	13.720683100697443
3	28.55749421672543	26.01054251202614	31.093132880972675	14.338830390275753
4	24.968432133311765	28.690284478129097	31.100238022232197	15.241045366326942
5	22.667677022352567	33.399544512157895	29.42721869245396	14.505559773035575
6	20.94723259921588	41.91888481013925	26.622515916723604	10.511366673921268
7	87.14090150446192	4.550670521838134	6.645480009202193	1.6629479644977476
8	87.88445798636847	3.5716096490577582	6.0836909760222175	2.460241388551548
9	81.95925304960596	5.8464275453220065	8.744428413804807	3.449890991267229
10	46.20514750238764	23.528675280903137	16.521867796775027	13.744309419934197
11	38.24897587666579	24.528430739876296	21.438522075432456	15.784071308025464
12	32.85182779758901	23.056838715707777	26.630483332407824	17.460850154295386
13	21.7281428092004	34.67595209755152	27.508347678705512	16.08755741454257
14	18.65568657911247	35.929837074972006	28.394731296319225	17.0197450495963
15	17.259491830639647	25.60127257908695	40.90253919123488	16.23669639903853
16	18.983730261245007	21.44200566410824	39.47819980553987	20.09606426910688
17	18.925440510135346	23.226051448810733	28.44315662801033	29.40535141304359
18	22.017004741474608	24.43020043741457	32.055155211121	21.49763960998983
19	26.78907284459849	26.848673252833695	25.916796036572855	20.445457865994968
20	28.329060476134142	25.062006153880112	25.747755758354835	20.86117761163091
21	23.106229794754544	28.981664251723426	27.03806320747461	20.87404274604742
22	23.67429618574633	26.143056845613906	25.648387253652512	24.53425971498726
23	21.10827097077269	29.720840379552506	25.223699853999694	23.947188795675107
24	21.421966406478234	25.766691304721228	33.806848459408776	19.004493829391762
25	19.693451094726363	27.406944206360894	31.21467908387826	21.68492561503448
26	20.013389397266728	31.08657959534496	27.389181353212095	21.51084965417622
27	19.871252081099325	31.459323583653898	27.471200896489474	21.198223438757307
28	18.848146230705318	28.493375490504807	33.41509994277947	19.24337833601041
29	19.357508978863585	25.7831090097675	32.10226988568656	22.75711212568236
30	20.714625450409045	29.046024414782973	29.37248151197406	20.866868622833923
31	25.824705128014987	27.35227600783497	25.79731929228654	21.025699571863502
32	26.31940921095337	26.81076766912392	26.793694635514875	20.07612848440784
33	26.021752079547227	27.487998002282616	25.575438837322963	20.914811080847194
34	20.607013602206596	27.1071486343815	28.338752440667758	23.947085322744144
35	21.294625719438667	26.503349591230187	29.895157777249683	22.306866912081468
36	26.500107439393318	26.40480886997557	26.44554271379836	20.649540976832757
37	21.848688773773336	29.97065852587634	28.76009421555274	19.420558484797585
38	21.245131167460936	30.446392571471343	26.321306214687706	21.987170046380015
39	20.776881663872324	30.13742239961315	27.325648973600263	21.760046962914267
40	22.99203016994739	26.87812854718141	26.78155381161511	23.348287471256082
41	19.256484907265833	25.258639213688504	27.68618315605546	27.798692722990204
42	21.668852819758087	27.431053399275484	25.845330732253792	25.05476304871264
43	22.483150295466956	26.75354713830089	27.181994054445386	23.581308511786776
44	20.739148535047487	29.50099489223122	27.74350715980946	22.016349412911833
45	19.244413065320046	32.13989954158043	25.521529440290774	23.094157952808754
46	22.390473040300293	29.438635205836977	26.635898415794934	21.534993338067796
47	22.009106307744364	27.22717723429962	27.946314104498693	22.817402353457325
48	23.09850381590924	25.01713339281877	28.885124007134117	22.999238784137876
49	21.48408465603356	24.87727248113257	29.715873678866238	23.92276918396763
50	20.364714488866138	29.607261592331135	28.332578555786913	21.695445363015807
51	19.555176767981614	30.30487609288972	26.353141386447597	23.78680575268107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2578.0
1	2871.5
2	3165.0
3	14607.5
4	26050.0
5	17890.0
6	9730.0
7	10535.5
8	11341.0
9	12664.0
10	13987.0
11	15172.5
12	16358.0
13	16778.5
14	17199.0
15	16984.5
16	16770.0
17	16154.0
18	15538.0
19	15131.0
20	14724.0
21	15138.5
22	15553.0
23	13893.0
24	12233.0
25	15894.5
26	22588.5
27	25621.0
28	29529.5
29	33438.0
30	37361.0
31	41284.0
32	47921.5
33	54559.0
34	61448.0
35	68337.0
36	77619.0
37	86901.0
38	100843.0
39	114785.0
40	141894.0
41	169003.0
42	195691.5
43	222380.0
44	232925.5
45	243471.0
46	252141.0
47	260811.0
48	255751.5
49	250692.0
50	236611.0
51	222530.0
52	208114.0
53	193698.0
54	174716.0
55	155734.0
56	143220.0
57	130706.0
58	120595.0
59	110484.0
60	99230.5
61	87977.0
62	80245.5
63	72514.0
64	62189.5
65	51865.0
66	44428.5
67	36992.0
68	30071.5
69	23151.0
70	20303.0
71	17455.0
72	15175.0
73	12895.0
74	10108.0
75	5838.0
76	4355.0
77	3694.5
78	3034.0
79	2235.5
80	1437.0
81	967.5
82	498.0
83	392.0
84	286.0
85	243.5
86	201.0
87	124.5
88	48.0
89	47.0
90	46.0
91	28.0
92	10.0
93	6.0
94	2.0
95	1.5
96	1.0
97	3.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2899309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.739672457268917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2538303517359	16.64438154936229
2	7.389765365080245	3.0652262641566903
3	2.4269540746637674	1.5100269773208208
4	1.2970380972499724	1.0760058120625493
5	0.8154449983434269	0.8456031086280433
6	0.5809262164951171	0.7228931671169531
7	0.4586632545308427	0.6658767967008248
8	0.3549737477552134	0.5889631407497858
9	0.2971577122947557	0.5546658259030126
>10	3.5705505717394823	17.69882352097242
>50	1.4406884503244424	21.76166868570547
>100	1.1094554053464938	33.23176832814725
>500	0.0025287524668195023	0.3390076983438126
>1k	0.0016858349778796679	0.49529528204810747
>5k	1.685834977879668E-4	0.18499275288130287
>10k+	1.685834977879668E-4	0.6148010899007428
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17584	0.6064893393563777	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5291	0.18249175924332314	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.898195397593012E-5	0.0	0.0	0.04356210393579987	0.0
2	6.898195397593012E-5	0.0	0.0	0.17628338338548943	0.0
3	6.898195397593012E-5	0.0	0.0	0.27065069642456185	0.0
4	6.898195397593012E-5	0.0	0.0	0.4720090200803019	0.0
5	6.898195397593012E-5	0.0	0.0	0.7973624060077763	0.0
6	6.898195397593012E-5	0.0	0.0	1.4785592015200864	0.0
7	6.898195397593012E-5	0.0	0.0	1.7835974020016494	0.0
8	6.898195397593012E-5	0.0	0.0	2.6098977377023282	0.0
9	6.898195397593012E-5	0.0	0.0	3.0166498293214006	0.0
10	1.3796390795186024E-4	0.0	0.0	3.625863955859827	0.0
11	1.3796390795186024E-4	0.0	0.0	4.069693847740962	0.0
12	1.3796390795186024E-4	0.0	0.0	4.458062248625448	0.0
13	1.3796390795186024E-4	0.0	0.0	4.667077569172517	0.0
14	1.3796390795186024E-4	0.0	0.0	4.764273142324602	0.0
15	1.3796390795186024E-4	0.0	0.0	4.864055538750785	0.0
16	1.3796390795186024E-4	0.0	0.0	5.040545867998202	0.0
17	1.3796390795186024E-4	0.0	0.0	5.227211035457069	0.0
18	1.3796390795186024E-4	0.0	0.0	5.51262387003248	0.0
19	1.3796390795186024E-4	0.0	0.0	5.63668791425819	0.0
20	2.069458619277904E-4	0.0	0.0	5.771547634281134	0.0
21	2.069458619277904E-4	0.0	0.0	5.93534528399698	0.0
22	3.1041879289168554E-4	0.0	0.0	6.109179808016324	0.0
23	3.1041879289168554E-4	0.0	0.0	6.309261965523509	0.0
24	3.1041879289168554E-4	0.0	0.0	6.478957572304298	0.0
25	3.1041879289168554E-4	0.0	0.0	6.622612491459172	0.0
26	3.1041879289168554E-4	0.0	0.0	6.751229344647293	0.0
27	3.1041879289168554E-4	0.0	0.0	6.886054573693249	0.0
28	3.1041879289168554E-4	0.0	0.0	7.023811535783182	0.0
29	3.1041879289168554E-4	0.0	0.0	7.177986202919385	0.0
30	3.1041879289168554E-4	0.0	0.0	7.385104519732115	0.0
31	5.17364654819476E-4	0.0	0.0	7.555558928006639	0.0
32	5.17364654819476E-4	0.0	0.0	7.7137000574964585	0.0
33	5.17364654819476E-4	0.0	0.0	7.869840710320976	0.0
34	5.17364654819476E-4	0.0	0.0	8.030016807453086	0.0
35	5.17364654819476E-4	0.0	0.0	8.2211313109434	0.0
36	5.17364654819476E-4	0.0	0.0	8.381686808822378	0.0
37	5.17364654819476E-4	0.0	0.0	8.54979583066172	0.0
38	5.17364654819476E-4	0.0	0.0	8.719250000603592	0.0
39	5.17364654819476E-4	0.0	0.0	8.896361167436792	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAA	25	3.8922815E-5	45.0	36
CGTTTTT	9950	0.0	41.811558	1
ACGTATG	70	0.0	41.785713	1
TACGAAT	280	0.0	40.17857	12
TACGGGA	770	0.0	40.032467	4
GCGTAAT	45	1.9301297E-8	40.0	23
CGTAAGG	395	0.0	39.3038	2
GTACGGG	1070	0.0	39.11215	3
CGTTAGG	455	0.0	39.065933	2
GCGATCG	145	0.0	38.793106	9
GCTACGA	290	0.0	38.793106	10
TAGTACG	175	0.0	38.571426	1
AGGGCGA	3500	0.0	38.507145	6
GGGCGAT	7160	0.0	38.023743	7
TAAACGG	190	0.0	37.894737	2
AGTACGG	415	0.0	37.409637	2
GCGTTAG	265	0.0	37.35849	1
GCGAGAC	205	0.0	37.317074	21
ATACGGG	935	0.0	36.57754	3
TAGGGAC	2250	0.0	36.4	5
>>END_MODULE