FastQCFastQC Report
Thu 26 May 2016
SRR1547658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547658_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2405682
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391091.6256928388706404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG700.045.01
CGTTTTT261650.043.383341
TCTAGCG1300.039.8076931
GTTTTTT301550.038.3294682
TTCGTAG900.037.5000041
GTATGCG900.037.5000041
TTACGCG601.5643309E-1037.4999961
TATGGGC9500.037.421054
AGGGCGA30700.037.304566
GGGCGAT59100.036.738587
TATCGCG1050.036.428571
TCGCGAT250.00210756635.99999622
GGCGATT15850.035.772878
TTACGGG7750.035.709683
GCGATAT3000.035.259
ACGGGTA3900.035.1923075
TACGGGA5700.035.131584
CGTAGGT458.705174E-735.0000043
ATACTAT12450.034.87951745
AAGGGCG11300.034.646025
Produced by FastQC (version 0.11.5)