##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547658_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2405682
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.491127671903435	31.0	31.0	33.0	30.0	34.0
2	31.912036171031748	31.0	31.0	34.0	30.0	34.0
3	31.95788512363646	31.0	31.0	34.0	30.0	34.0
4	35.70785914347782	37.0	35.0	37.0	33.0	37.0
5	35.626293500138424	37.0	35.0	37.0	33.0	37.0
6	35.67970330243149	37.0	35.0	37.0	33.0	37.0
7	36.05428273562341	37.0	35.0	37.0	35.0	37.0
8	36.06441042498551	37.0	35.0	37.0	35.0	37.0
9	37.811209045917124	39.0	38.0	39.0	35.0	39.0
10	37.2382800386751	39.0	37.0	39.0	34.0	39.0
11	37.01585122223137	39.0	37.0	39.0	33.0	39.0
12	36.69103688683708	39.0	35.0	39.0	32.0	39.0
13	36.615085451859386	39.0	35.0	39.0	32.0	39.0
14	37.768204608921714	40.0	36.0	41.0	33.0	41.0
15	37.844962468023624	40.0	36.0	41.0	33.0	41.0
16	37.86311781856455	40.0	36.0	41.0	33.0	41.0
17	37.75992920095008	40.0	36.0	41.0	33.0	41.0
18	37.52195427325806	39.0	36.0	41.0	32.0	41.0
19	37.369363864384404	39.0	36.0	41.0	32.0	41.0
20	37.15751125876155	39.0	35.0	41.0	32.0	41.0
21	37.04791406345477	39.0	35.0	41.0	32.0	41.0
22	36.96357373917251	38.0	35.0	41.0	32.0	41.0
23	36.84673784814452	38.0	35.0	41.0	32.0	41.0
24	36.70547354139076	38.0	35.0	40.0	31.0	41.0
25	36.580115326963416	38.0	35.0	40.0	31.0	41.0
26	36.41914891494387	38.0	35.0	40.0	31.0	41.0
27	36.367741039755046	38.0	35.0	40.0	31.0	41.0
28	36.35225935930019	38.0	35.0	40.0	31.0	41.0
29	36.237528484645935	38.0	35.0	40.0	30.0	41.0
30	36.19067399598118	38.0	35.0	40.0	30.0	41.0
31	35.988075315025014	38.0	35.0	40.0	30.0	41.0
32	35.62135145044108	38.0	35.0	40.0	28.0	41.0
33	35.21802964814136	38.0	34.0	40.0	25.0	41.0
34	34.73944685955999	38.0	34.0	40.0	23.0	41.0
35	34.425171323558146	38.0	34.0	40.0	21.0	41.0
36	34.23242972263167	38.0	33.0	40.0	20.0	41.0
37	33.979216704452206	37.0	33.0	40.0	18.0	41.0
38	34.00386418487564	37.0	33.0	40.0	18.0	41.0
39	33.86684690661526	37.0	33.0	40.0	18.0	41.0
40	33.72987867889439	37.0	33.0	40.0	18.0	41.0
41	33.62979437847562	37.0	33.0	40.0	18.0	41.0
42	33.56094654239421	37.0	33.0	40.0	17.0	41.0
43	33.46857730988551	36.0	33.0	40.0	17.0	41.0
44	33.30485325990717	36.0	33.0	40.0	16.0	41.0
45	33.23199408733158	36.0	33.0	40.0	17.0	41.0
46	33.04375765375474	35.0	32.0	40.0	15.0	41.0
47	33.04988065754327	35.0	32.0	40.0	16.0	41.0
48	32.87336148335483	35.0	32.0	40.0	15.0	41.0
49	32.826997915767755	35.0	32.0	40.0	15.0	41.0
50	32.71565693221299	35.0	32.0	40.0	15.0	41.0
51	32.05566737415835	35.0	31.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	11.0
14	41.0
15	90.0
16	247.0
17	527.0
18	1320.0
19	2657.0
20	4587.0
21	7351.0
22	11466.0
23	17483.0
24	27123.0
25	42453.0
26	60390.0
27	66070.0
28	61570.0
29	58171.0
30	61455.0
31	70551.0
32	84553.0
33	107172.0
34	165458.0
35	229844.0
36	205449.0
37	249646.0
38	339317.0
39	530351.0
40	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.33980301635877	22.236604838045928	25.791438768715068	20.632153376880236
2	38.474079283961885	23.618583004736287	25.42189699220429	12.485440719097536
3	27.135548256170182	24.029817739834275	36.00567323528214	12.828960768713404
4	23.69864346160465	26.781345165321103	35.429079986465375	14.09093138660887
5	21.61748726556544	30.043039770011166	34.42711879624988	13.912354168173517
6	20.214267721170128	38.425943246031686	31.565477066378683	9.794311966419501
7	81.2313098738736	3.6455774287707188	13.49280578231038	1.630306915045297
8	81.16758574075875	3.3815774487234807	13.454604557044531	1.9962322534732353
9	75.81126682578994	5.5038862160501685	15.458734778744656	3.2261121794152343
10	38.79082937811398	25.100283412354585	22.757953877528287	13.350933332003148
11	30.604585310943012	25.938340977735212	27.821923263340707	15.635150447981072
12	26.25716948457859	22.808750283703333	33.302406552486985	17.631673679231085
13	22.39963552955046	25.824776508283303	34.323946390254406	17.451641571911832
14	20.29328897169285	27.674314394005524	34.2105481938178	17.82184844048382
15	19.80020634481199	23.927435130661493	38.85056295886156	17.421795565664954
16	22.100011555974564	22.580997820992135	37.45607274776965	17.862917875263644
17	22.163901962104717	23.227633577505255	33.24030358127134	21.368160879118687
18	23.992198470121988	23.47093256714728	33.99460111519311	18.542267847537623
19	25.680950350046267	25.57557482659803	30.45589566700836	18.28757915634735
20	27.26233143033867	24.307161129359574	30.214300975773188	18.21620646452856
21	24.81919887998497	25.544523340990207	31.351649968699103	18.28462781032572
22	24.297392589710526	23.9334209592124	31.297819079994778	20.471367371082298
23	22.557511757580595	26.338476989061732	30.818453976876413	20.285557276481264
24	22.370288342349486	25.11329427580204	33.665172703624165	18.851244678224305
25	22.06833654655935	25.89178453345039	32.272553063954426	19.767325856035836
26	20.80790395405544	27.39767766479526	31.358550298834174	20.43586808231512
27	20.921385286999694	26.24519782747678	32.23559888630334	20.59781799922018
28	20.719779255944886	26.883145818940324	33.6976790781159	18.699395846998897
29	21.753373887321768	24.99802550794328	33.33657565713174	19.912024947603214
30	22.304070114005093	25.332982497271043	33.39306691408092	18.96988047464295
31	24.58949270934396	25.732578121297827	30.162714772775455	19.515214396582756
32	24.941201704963497	26.050367421795567	30.014482379632884	18.99394849360805
33	24.41661034168273	26.60692477226832	29.184987874540358	19.791477011508587
34	22.577298246401647	27.65934982262826	30.425135159177312	19.33821677179278
35	22.990154143398836	27.745229835032227	29.32469877564865	19.939917245920284
36	24.77330752776136	28.411236397828144	27.320693258710005	19.494762815700494
37	23.646392166545706	28.64023590815411	28.154427725692756	19.558944199607428
38	23.637205582450214	28.942062999182767	27.24420767167065	20.17652374669636
39	23.394280707092623	28.796324701269743	26.7600206511085	21.049373940529133
40	24.273033592968645	27.188589348051824	27.576836838784182	20.961540220195353
41	21.680587874872906	27.45674615348163	27.953237377176205	22.90942859446926
42	23.00337284811542	28.278966214154654	26.709182676679628	22.008478261050296
43	22.998343089402507	27.117008814963906	27.8555104124319	22.029137683201686
44	22.63345695732021	28.40283961055534	27.69501538441074	21.268688047713706
45	21.615990808427714	29.17085466823961	27.31977875712584	21.893375766206837
46	23.02823066390321	28.72037950153013	27.200935119438064	21.0504547151286
47	22.356071999541086	28.648092308127175	28.128156589274894	20.867679103056847
48	23.040451730527973	27.313959201590237	28.534694111690573	21.110894956191217
49	22.588355401919287	27.054282319940874	28.371330874155436	21.9860314039844
50	21.672315792361584	28.323984633047928	28.634582625633815	21.36911694895668
51	21.388570891747122	28.955697386437606	27.553392343626466	22.102339378188805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1746.0
1	2363.0
2	2980.0
3	31241.5
4	59503.0
5	41121.5
6	22740.0
7	23716.5
8	24693.0
9	26378.0
10	28063.0
11	29454.0
12	30845.0
13	31255.0
14	31665.0
15	30748.0
16	29831.0
17	28453.0
18	27075.0
19	25665.0
20	24255.0
21	23361.0
22	22467.0
23	21651.5
24	20836.0
25	20783.0
26	22805.0
27	24880.0
28	26361.5
29	27843.0
30	31634.5
31	35426.0
32	38965.0
33	42504.0
34	49480.0
35	56456.0
36	61072.5
37	65689.0
38	72998.5
39	80308.0
40	90624.0
41	100940.0
42	111516.5
43	122093.0
44	127345.0
45	132597.0
46	137129.5
47	141662.0
48	148114.5
49	154567.0
50	158084.0
51	161601.0
52	157554.0
53	153507.0
54	143856.0
55	134205.0
56	123865.0
57	113525.0
58	111529.5
59	109534.0
60	102254.5
61	94975.0
62	88832.0
63	82689.0
64	73727.0
65	64765.0
66	56904.5
67	49044.0
68	41955.5
69	34867.0
70	30375.5
71	25884.0
72	21714.0
73	17544.0
74	15427.0
75	11100.5
76	8891.0
77	6786.5
78	4682.0
79	3528.5
80	2375.0
81	1692.0
82	1009.0
83	785.0
84	561.0
85	349.5
86	138.0
87	118.0
88	98.0
89	68.0
90	38.0
91	31.0
92	24.0
93	20.0
94	16.0
95	10.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2405682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.558614501342312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55701936576132	22.629194228043364
2	6.86195340949187	4.056596711146835
3	3.1056342579543363	2.7539473743910357
4	1.760777989494945	2.0818463125571856
5	1.271246999290487	1.8788149994007846
6	0.9847428865160742	1.7464581219280633
7	0.7978170366669513	1.6507656360609186
8	0.659596506374022	1.5597407086673516
9	0.5872511254866976	1.5622496670366628
>10	6.766500100629156	44.37651836947574
>50	0.5925180270599656	11.233515471275043
>100	0.052807790657093706	2.360732819194212
>500	0.0015653033858534227	0.3016686598277685
>1k	4.2690092341456977E-4	0.16294818574634348
>5k	0.0	0.0
>10k+	1.423003078048566E-4	1.6450027352487728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39109	1.6256928388706404	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02814170784002208	0.0
2	0.0	0.0	0.0	0.08866508541029114	0.0
3	0.0	0.0	0.0	0.16993102163960158	0.0
4	0.0	0.0	0.0	0.24130371345838728	0.0
5	0.0	0.0	0.0	0.38176284313554326	0.0
6	0.0	0.0	0.0	0.7735436354430885	0.0
7	0.0	0.0	0.0	0.9987188664170908	0.0
8	0.0	0.0	0.0	1.504853924999231	0.0
9	4.156825382573424E-5	0.0	0.0	1.887281860195986	0.0
10	8.313650765146848E-5	0.0	0.0	2.3876804997501746	0.0
11	8.313650765146848E-5	0.0	0.0	2.715820295450521	0.0
12	8.313650765146848E-5	0.0	0.0	3.0134074245889524	0.0
13	8.313650765146848E-5	0.0	0.0	3.242739480945528	0.0
14	8.313650765146848E-5	0.0	0.0	3.3854848645830997	0.0
15	8.313650765146848E-5	0.0	0.0	3.4845004451959984	0.0
16	8.313650765146848E-5	0.0	0.0	3.604217016214113	0.0
17	8.313650765146848E-5	0.0	0.0	3.717033257097156	0.0
18	8.313650765146848E-5	0.0	0.0	3.8858003676296367	0.0
19	8.313650765146848E-5	0.0	0.0	3.9825296942821202	0.0
20	1.6627301530293696E-4	0.0	0.0	4.086491897100282	0.0
21	1.6627301530293696E-4	0.0	0.0	4.195068176093099	0.0
22	2.078412691286712E-4	0.0	0.0	4.307260893168756	0.0
23	2.078412691286712E-4	0.0	0.0	4.430510765762058	0.0
24	2.078412691286712E-4	0.0	0.0	4.549645381226613	0.0
25	2.078412691286712E-4	0.0	0.0	4.651778580876441	0.0
26	2.078412691286712E-4	0.0	0.0	4.75129298053525	0.0
27	2.078412691286712E-4	0.0	0.0	4.868764865846774	0.0
28	2.078412691286712E-4	0.0	0.0	4.975470573417434	0.0
29	2.078412691286712E-4	0.0	0.0	5.0860421285938875	0.0
30	2.4940952295440544E-4	0.0	0.0	5.235355296335925	0.0
31	2.4940952295440544E-4	0.0	0.0	5.357358121314455	0.0
32	2.4940952295440544E-4	0.0	0.0	5.505673650964675	0.0
33	2.4940952295440544E-4	0.0	0.0	5.6253070854751375	0.0
34	2.4940952295440544E-4	0.0	0.0	5.751092621551809	0.0
35	2.4940952295440544E-4	0.0	0.0	5.885981605216317	0.0
36	2.4940952295440544E-4	0.0	0.0	6.0160902396908655	0.0
37	2.4940952295440544E-4	0.0	0.0	6.148069445587572	0.0
38	2.4940952295440544E-4	0.0	0.0	6.297299476821958	0.0
39	2.4940952295440544E-4	0.0	0.0	6.481613114285263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	70	0.0	45.0	1
CGTTTTT	26165	0.0	43.38334	1
TCTAGCG	130	0.0	39.807693	1
GTTTTTT	30155	0.0	38.329468	2
TTCGTAG	90	0.0	37.500004	1
GTATGCG	90	0.0	37.500004	1
TTACGCG	60	1.5643309E-10	37.499996	1
TATGGGC	950	0.0	37.42105	4
AGGGCGA	3070	0.0	37.30456	6
GGGCGAT	5910	0.0	36.73858	7
TATCGCG	105	0.0	36.42857	1
TCGCGAT	25	0.002107566	35.999996	22
GGCGATT	1585	0.0	35.77287	8
TTACGGG	775	0.0	35.70968	3
GCGATAT	300	0.0	35.25	9
ACGGGTA	390	0.0	35.192307	5
TACGGGA	570	0.0	35.13158	4
CGTAGGT	45	8.705174E-7	35.000004	3
ATACTAT	1245	0.0	34.879517	45
AAGGGCG	1130	0.0	34.64602	5
>>END_MODULE