FastQCFastQC Report
Thu 26 May 2016
SRR1547657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547657_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2949680
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156300.5298879878495294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106300.040.872531
AGGGCGA35100.040.512826
GGGCGAT74900.037.820437
CGTTAGG5950.037.4369742
TAGGGCG14050.037.3131685
GGCGATA15100.037.2516568
ATAGGGC15150.036.9801984
TAAGGGA28700.036.9250874
CGTTGAT2500.036.925
TACGGGA7650.036.7647064
CACGACC13450.036.468427
TAACGGG8650.036.4161873
TATGGGC13100.036.4122124
AGGGTAC13050.036.379316
AAGGGAT30550.036.088385
TCGTTAG1450.035.6896551
GGCACCG17000.035.602948
GTAGGGC20050.035.5735664
ATAGGGA32100.035.5373844
AACACGT12350.035.52631841
Produced by FastQC (version 0.11.5)