##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547657_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2949680
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.401215047055953	31.0	31.0	33.0	30.0	34.0
2	31.81354079086545	31.0	31.0	34.0	30.0	34.0
3	31.964688033956225	31.0	31.0	34.0	30.0	34.0
4	35.662846817281874	37.0	35.0	37.0	33.0	37.0
5	35.54116446529793	37.0	35.0	37.0	33.0	37.0
6	35.5879532695072	37.0	35.0	37.0	33.0	37.0
7	35.986653467494776	37.0	35.0	37.0	35.0	37.0
8	35.9976366927938	37.0	35.0	37.0	35.0	37.0
9	37.74764415122996	39.0	37.0	39.0	35.0	39.0
10	37.116701472702125	39.0	37.0	39.0	33.0	39.0
11	36.857438773019446	39.0	37.0	39.0	32.0	39.0
12	36.38906389845678	38.0	35.0	39.0	32.0	39.0
13	36.21171618616257	39.0	35.0	39.0	32.0	39.0
14	37.26807450299694	39.0	35.0	41.0	32.0	41.0
15	37.41798161156464	39.0	35.0	41.0	32.0	41.0
16	37.48097725855008	39.0	35.0	41.0	32.0	41.0
17	37.392387648829704	39.0	35.0	41.0	32.0	41.0
18	37.281184399663694	39.0	35.0	41.0	32.0	41.0
19	37.25146287054867	39.0	35.0	41.0	32.0	41.0
20	37.15841616717746	39.0	35.0	41.0	32.0	41.0
21	37.04848525941797	39.0	35.0	41.0	32.0	41.0
22	36.94888292967373	39.0	35.0	41.0	31.0	41.0
23	36.84798859537306	38.0	35.0	41.0	31.0	41.0
24	36.728579710341464	38.0	35.0	41.0	31.0	41.0
25	36.58713012936997	38.0	35.0	40.0	31.0	41.0
26	36.418571506061674	38.0	35.0	40.0	31.0	41.0
27	36.392694122752296	38.0	35.0	40.0	30.0	41.0
28	36.40677870141846	38.0	35.0	40.0	30.0	41.0
29	36.394864866697404	38.0	35.0	41.0	30.0	41.0
30	36.40228363754712	38.0	35.0	40.0	30.0	41.0
31	36.28660668275881	38.0	35.0	41.0	30.0	41.0
32	36.09638401453717	38.0	35.0	41.0	30.0	41.0
33	35.98686637194543	38.0	35.0	41.0	30.0	41.0
34	35.77872921808467	38.0	35.0	40.0	29.0	41.0
35	35.68167767351035	38.0	35.0	40.0	28.0	41.0
36	35.53269202082938	38.0	35.0	40.0	27.0	41.0
37	35.290147066800465	38.0	34.0	40.0	26.0	41.0
38	35.34957893737626	38.0	34.0	40.0	27.0	41.0
39	35.23831703778037	38.0	34.0	40.0	26.0	41.0
40	35.09749464348675	38.0	34.0	40.0	26.0	41.0
41	35.04012977678935	37.0	34.0	40.0	26.0	41.0
42	34.99548256082016	37.0	34.0	40.0	26.0	41.0
43	34.91152192780234	37.0	34.0	40.0	26.0	41.0
44	34.75221244338369	37.0	34.0	40.0	25.0	41.0
45	34.68226349976947	37.0	34.0	40.0	25.0	41.0
46	34.49817675137642	36.0	34.0	40.0	24.0	41.0
47	34.51488398741559	36.0	33.0	40.0	24.0	41.0
48	34.338862859700036	36.0	33.0	40.0	24.0	41.0
49	34.30944271921022	36.0	33.0	40.0	24.0	41.0
50	34.16178534620705	36.0	33.0	40.0	24.0	41.0
51	33.45931660383499	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	5.0
13	15.0
14	43.0
15	111.0
16	255.0
17	658.0
18	1384.0
19	2734.0
20	4711.0
21	7479.0
22	11357.0
23	16533.0
24	23528.0
25	32597.0
26	42293.0
27	50115.0
28	56300.0
29	64168.0
30	74544.0
31	89411.0
32	109352.0
33	140269.0
34	223639.0
35	323685.0
36	233797.0
37	297384.0
38	430514.0
39	712427.0
40	365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37795964307993	23.81261696183993	28.577642320522905	14.23178107455724
2	31.118663719454315	25.49683355482629	29.446482330286678	13.938020395432723
3	28.94419055626373	26.134936671096526	30.880163271948142	14.0407095006916
4	25.431470532396734	28.73142171354181	30.681497653982802	15.155610100078654
5	23.087589162214208	32.73748338802853	29.534966504841204	14.639960944916059
6	21.61939600227821	41.3059382712701	26.413475360039058	10.661190366412628
7	88.22177320929728	3.7577296520300507	6.342043882726261	1.6784532559464078
8	88.67704293347074	3.13776409644436	5.927897263431965	2.257295706652925
9	83.12437281332213	4.9603346803721085	8.610188223807329	3.3051042824984402
10	43.99534864798893	25.668920018442677	15.346613869979118	14.989117463589272
11	35.37041306175585	24.787095549347725	23.5034308806379	16.339060508258523
12	31.282240785441136	22.90536600580402	26.66590952238887	19.14648368636598
13	23.511296140598304	29.41234303382062	28.458680263621815	18.61768056195926
14	20.51734425429199	31.078523772070188	29.437566108866047	18.966565864771773
15	19.87168099590464	23.991382116026145	37.71168397927911	18.425252908790107
16	22.447350221040928	21.800534295245587	36.185484527135145	19.56663095657834
17	22.85213989314095	22.440230804697457	28.569878766510264	26.137750535651328
18	25.49137533562963	22.789794147161725	30.22965203005072	21.48917848715793
19	27.23942936182908	25.70248298120474	25.916234981421717	21.141852675544463
20	29.22744162078598	24.614161536166634	25.29037048086572	20.86802636218166
21	26.245660546228745	26.440122318353176	26.88925578367823	20.42496135173985
22	25.62688156003363	23.588016327194815	27.199289414444955	23.585812698326595
23	23.728709554934774	27.15077567736161	26.07526918174175	23.04524558596187
24	23.66487212172168	24.995457134333215	31.126664587345065	20.213006156600038
25	22.738432643540996	25.531074557240107	29.467840579317077	22.26265221990182
26	21.673062840714923	28.924696916275668	27.06764801605598	22.334592226953433
27	22.18491497382767	27.06958042906349	28.133390740690515	22.612113856418322
28	20.95067939573106	27.06893629139432	31.44161400558705	20.53877030728757
29	22.09575275962138	24.974268395215752	30.432453689891787	22.49752515527108
30	22.900145100485474	26.041774022944864	29.744277345339153	21.31380353123051
31	26.70109978031515	26.27932521493857	25.751878169835372	21.267696834910907
32	27.30096146022619	25.8774850153237	25.45201513384503	21.369538390605083
33	26.072828238995417	26.4186962653576	25.1546269425836	22.353848553063383
34	22.769927585365192	27.94370914811098	27.358221908817228	21.9281413577066
35	22.961473786987064	27.29743565403705	27.81098288627919	21.9301076726967
36	26.681233218518617	27.177490439640913	25.690617287298963	20.45065905454151
37	23.719250901792737	27.97340728485802	26.54779501505248	21.759546798296764
38	23.930019527541972	28.609849203981447	25.439403596322315	22.020727672154266
39	23.190922405142253	27.558107998155734	25.65603726505926	23.594932331642752
40	24.585616066827587	26.2145724281956	26.263018361313765	22.936793143663042
41	21.465887825120014	25.114520897182068	27.192678527840307	26.22691274985761
42	24.248291340077568	26.387574245341867	25.277826747308186	24.086307667272383
43	23.59069458381926	25.66641127173117	26.822638387892923	23.920255756556642
44	22.802439586667028	27.693648124542324	26.735442488676735	22.768469800113913
45	21.71940685091264	28.872657373003175	25.67695478831602	23.730980987768167
46	23.76895120826666	27.725007458436167	26.22247159013859	22.28356974315858
47	22.89183911475143	27.172133926392018	27.280823682568954	22.655203276287597
48	24.12404057389276	25.600777033440913	27.66225488866589	22.612927504000435
49	23.16705540940034	24.73814108649074	28.170445607659133	23.924357896449784
50	22.201120121504704	27.890415231482734	27.446367063545875	22.46209758346668
51	21.62621030077839	28.65402348729354	25.65603726505926	24.063728946868814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1759.0
1	2389.0
2	3019.0
3	14544.0
4	26069.0
5	18358.0
6	10647.0
7	11346.0
8	12045.0
9	12871.0
10	13697.0
11	13893.0
12	14089.0
13	14328.5
14	14568.0
15	14450.0
16	14332.0
17	13615.5
18	12899.0
19	12414.5
20	11930.0
21	12557.5
22	13185.0
23	14306.5
24	15428.0
25	15836.5
26	18392.5
27	20540.0
28	24684.0
29	28828.0
30	32597.0
31	36366.0
32	44594.5
33	52823.0
34	58890.0
35	64957.0
36	75004.5
37	85052.0
38	91552.5
39	98053.0
40	122192.0
41	146331.0
42	165873.5
43	185416.0
44	193204.0
45	200992.0
46	204613.5
47	208235.0
48	217349.0
49	226463.0
50	229815.0
51	233167.0
52	226926.5
53	220686.0
54	205007.0
55	189328.0
56	175164.0
57	161000.0
58	151215.5
59	141431.0
60	132026.5
61	122622.0
62	112650.0
63	102678.0
64	87887.5
65	73097.0
66	64628.0
67	56159.0
68	46621.0
69	37083.0
70	31740.0
71	26397.0
72	23026.5
73	19656.0
74	17173.5
75	11482.5
76	8274.0
77	6368.0
78	4462.0
79	3528.0
80	2594.0
81	2004.5
82	1415.0
83	999.5
84	584.0
85	404.0
86	224.0
87	154.0
88	84.0
89	73.0
90	62.0
91	34.5
92	7.0
93	7.5
94	8.0
95	4.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2949680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.263746117956984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74778966038764	16.1598896314633
2	6.204382470311696	2.5144806239419797
3	2.2249421085764673	1.3525698604603624
4	1.1827273967608594	0.9586595077885691
5	0.821392426239748	0.832224379426748
6	0.588260259167564	0.7152213925833056
7	0.46140931847089606	0.6544916900167657
8	0.40713275369116597	0.6600027805682
9	0.3452565493933965	0.6296571956222695
>10	5.208576363321715	26.139045657211728
>50	2.1378850430820604	31.01792024980115
>100	0.6678629460840282	17.379356186353604
>500	0.0013615454358808204	0.1767140207160167
>1k	8.509658974255127E-4	0.2707269833373792
>5k	0.0	0.0
>10k+	1.7019317948510255E-4	0.5390398407086926
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15630	0.5298879878495294	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0170594776382523E-4	0.0	0.0	0.022477014455805376	0.0
2	1.0170594776382523E-4	0.0	0.0	0.07665238263133628	0.0
3	1.0170594776382523E-4	0.0	0.0	0.12781047435654036	0.0
4	1.0170594776382523E-4	0.0	0.0	0.2576211656857693	0.0
5	1.0170594776382523E-4	0.0	0.0	0.4323180819614331	0.0
6	1.0170594776382523E-4	0.0	0.0	0.957866616039706	0.0
7	1.0170594776382523E-4	0.0	0.0	1.219115293862385	0.0
8	1.0170594776382523E-4	0.0	0.0	1.950584470179816	0.0
9	1.0170594776382523E-4	0.0	0.0	2.3540180629763228	0.0
10	1.0170594776382523E-4	0.0	0.0	2.886889425293591	0.0
11	1.0170594776382523E-4	0.0	0.0	3.218213501125546	0.0
12	1.0170594776382523E-4	0.0	0.0	3.500854329961216	0.0
13	1.0170594776382523E-4	0.0	0.0	3.6716864202218544	0.0
14	1.0170594776382523E-4	0.0	0.0	3.761085948306257	0.0
15	1.0170594776382523E-4	0.0	0.0	3.829805267012015	0.0
16	1.0170594776382523E-4	0.0	0.0	3.9365965121640314	0.0
17	1.0170594776382523E-4	0.0	0.0	4.045625288166852	0.0
18	1.0170594776382523E-4	0.0	0.0	4.211914512760706	0.0
19	1.0170594776382523E-4	0.0	0.0	4.296635567247972	0.0
20	1.0170594776382523E-4	0.0	0.0	4.389391391608581	0.0
21	1.0170594776382523E-4	0.0	0.0	4.493877301944618	0.0
22	1.3560793035176697E-4	0.0	0.0	4.608330395161509	0.0
23	1.3560793035176697E-4	0.0	0.0	4.738988636055437	0.0
24	1.3560793035176697E-4	0.0	0.0	4.846525724824388	0.0
25	1.3560793035176697E-4	0.0	0.0	4.939790078923815	0.0
26	1.6950991293970873E-4	0.0	0.0	5.032715413197364	0.0
27	1.6950991293970873E-4	0.0	0.0	5.130590436928752	0.0
28	1.6950991293970873E-4	0.0	0.0	5.22927910824225	0.0
29	1.6950991293970873E-4	0.0	0.0	5.34013859130482	0.0
30	1.6950991293970873E-4	0.0	0.0	5.501512028423422	0.0
31	1.6950991293970873E-4	0.0	0.0	5.624576225217651	0.0
32	1.6950991293970873E-4	0.0	0.0	5.761167313064468	0.0
33	1.6950991293970873E-4	0.0	0.0	5.882909332537767	0.0
34	1.6950991293970873E-4	0.0	0.0	6.002379919177674	0.0
35	1.6950991293970873E-4	0.0	0.0	6.127410430962002	0.0
36	1.6950991293970873E-4	0.0	0.0	6.254203845840904	0.0
37	1.6950991293970873E-4	0.0	0.0	6.386896205690109	0.0
38	1.6950991293970873E-4	0.0	0.0	6.5379634401019775	0.0
39	1.6950991293970873E-4	0.0	0.0	6.760563857774403	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10630	0.0	40.87253	1
AGGGCGA	3510	0.0	40.51282	6
GGGCGAT	7490	0.0	37.82043	7
CGTTAGG	595	0.0	37.436974	2
TAGGGCG	1405	0.0	37.313168	5
GGCGATA	1510	0.0	37.251656	8
ATAGGGC	1515	0.0	36.980198	4
TAAGGGA	2870	0.0	36.925087	4
CGTTGAT	250	0.0	36.9	25
TACGGGA	765	0.0	36.764706	4
CACGACC	1345	0.0	36.4684	27
TAACGGG	865	0.0	36.416187	3
TATGGGC	1310	0.0	36.412212	4
AGGGTAC	1305	0.0	36.37931	6
AAGGGAT	3055	0.0	36.08838	5
TCGTTAG	145	0.0	35.689655	1
GGCACCG	1700	0.0	35.60294	8
GTAGGGC	2005	0.0	35.573566	4
ATAGGGA	3210	0.0	35.537384	4
AACACGT	1235	0.0	35.526318	41
>>END_MODULE