FastQCFastQC Report
Thu 26 May 2016
SRR1547656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547656_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3891675
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1351773.473491491452909No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT97570.25071466656388314No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT77880.20011948582551214No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC60560.1556142278067927No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG43570.11195693371106272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT694400.044.225591
GTTTTTT757500.040.5594062
TACGCGG2850.038.684212
AGGGCGA44350.038.0496066
GCGATAA4050.037.777789
TAGGGTA21450.037.6573455
GCGATAC3200.037.265639
CGTAAGG3950.037.025322
CGGGTAT5300.036.9339646
TAGGGCG17800.036.7837075
TACGGGT4050.036.6666684
GTTGATC13600.036.562516
CGTTCGG7650.036.4705945
AGGGTAC17600.036.4346586
CGTTTTC11550.036.233771
GGGCGAT90650.036.0397157
ACGGGTA5500.035.9999965
TAGGGTC10450.035.9569365
CGTTAGG4950.035.9090922
TAGGGAC29650.035.893765