FastQCFastQC Report
Thu 26 May 2016
SRR1547655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547655_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences629090
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69101.0984119919248438No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG207.0308486E-445.01
CCGTAAG207.0308486E-445.01
CGATCGA253.888786E-544.99999610
CGTTTTT55700.043.2226221
CGTCATA900.040.00000438
TTCGACG750.039.025
CGTTAGG1100.038.8636362
AGGGCGA8200.038.6890266
GCGATAC356.243781E-638.5714269
CGATAAT356.243781E-638.57142610
GCGATTC659.094947E-1238.0769279
AGTAGCG659.094947E-1238.0769271
TAGGGCG3550.038.028175
GTTTTTT64700.038.0100482
AGGCGTC950.037.89473735
TTAAGCG301.1393344E-437.5000041
ACTACGG301.1393344E-437.5000042
GTCGTTT301.1393344E-437.5000049
ATAGGGC3650.036.9862984
GGGCGAT16100.036.754667
Produced by FastQC (version 0.11.5)