FastQCFastQC Report
Thu 26 May 2016
SRR1547654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547654_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7063547
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518180.7335974404927157No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC81150.11488562332777003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG6950.041.115112
CGTTTTT287050.039.9991261
TATAGCG3400.038.382351
GTTGATC46100.037.5325416
CTACGTT601.5643309E-1037.50000416
GGGCGAT189950.036.9570967
CATATGC45100.036.81818433
GGTACCT50650.036.6929938
TCGTTAG2650.036.5094341
ATATGCT44800.036.4620534
TCTAACG1050.036.428571
GGCGATA38000.036.4144748
TACGGGT5500.036.4090924
ACGGGAT14600.036.3698655
TAGCATA46050.036.3517930
CAGTAGC47800.036.33891327
ACTACGG5400.036.2500042
ATGGGCG30000.035.9255
GTAGCAT47550.035.91482529
GGGTACC56600.035.8966457
Produced by FastQC (version 0.11.5)