FastQCFastQC Report
Thu 26 May 2016
SRR1547653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547653_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3609027
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348400.9653571447373489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT286950.042.5849461
AGGGCGA51800.038.6148646
TAGGGCG21550.037.7958225
TACGGGA9900.037.7272724
GGGCGAT100750.036.8486337
AAGGGCG21150.036.4893655
AGCAACG17950.036.47632210
CACGACC36550.036.38166427
GTTTTTT345200.036.36372
TATGGGC18100.036.2983444
GCGAGAC36650.036.2210121
ACGGGAT8900.036.1516845
TATACGG3550.036.1267622
AGACACG36600.036.0245924
TAGGGAC27250.035.917435
ACACGAC36900.035.7317126
GACACGA36900.035.7317125
GACCGAT28450.035.667849
TCAAGCG37800.035.5952417
CGAGACA37500.035.5222
Produced by FastQC (version 0.11.5)