##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547653_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3609027
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.443735943233452	31.0	31.0	33.0	30.0	34.0
2	31.862949764576435	31.0	31.0	34.0	30.0	34.0
3	31.958657555069554	31.0	31.0	34.0	30.0	34.0
4	35.68257788040932	37.0	35.0	37.0	33.0	37.0
5	35.58561961437252	37.0	35.0	37.0	33.0	37.0
6	35.630991954341155	37.0	35.0	37.0	33.0	37.0
7	36.02197489794341	37.0	35.0	37.0	35.0	37.0
8	36.03130095729403	37.0	35.0	37.0	35.0	37.0
9	37.775759505262776	39.0	38.0	39.0	35.0	39.0
10	37.146281532390866	39.0	37.0	39.0	33.0	39.0
11	36.96376641127927	39.0	37.0	39.0	33.0	39.0
12	36.62832087429659	39.0	35.0	39.0	32.0	39.0
13	36.524554956225046	39.0	35.0	39.0	32.0	39.0
14	37.64397938835038	40.0	36.0	41.0	32.0	41.0
15	37.72916883137754	40.0	36.0	41.0	33.0	41.0
16	37.74941140645387	40.0	36.0	41.0	33.0	41.0
17	37.636300864471224	40.0	36.0	41.0	32.0	41.0
18	37.47221674983313	39.0	36.0	41.0	32.0	41.0
19	37.36998365487429	39.0	36.0	41.0	32.0	41.0
20	37.20426502766535	39.0	35.0	41.0	32.0	41.0
21	37.095647940566806	39.0	35.0	41.0	32.0	41.0
22	36.99254480501254	39.0	35.0	41.0	32.0	41.0
23	36.87834061645978	38.0	35.0	41.0	31.0	41.0
24	36.74850340548851	38.0	35.0	41.0	31.0	41.0
25	36.6271341278411	38.0	35.0	40.0	31.0	41.0
26	36.4703375729802	38.0	35.0	40.0	31.0	41.0
27	36.452704011358186	38.0	35.0	40.0	31.0	41.0
28	36.44317207934438	38.0	35.0	40.0	31.0	41.0
29	36.38261614557054	38.0	35.0	41.0	30.0	41.0
30	36.36126745518945	38.0	35.0	41.0	30.0	41.0
31	36.2010162295821	38.0	35.0	41.0	30.0	41.0
32	35.90709351855777	38.0	35.0	41.0	29.0	41.0
33	35.62009289484396	38.0	35.0	41.0	27.0	41.0
34	35.25768496605872	38.0	34.0	41.0	25.0	41.0
35	35.01522709583497	38.0	34.0	40.0	24.0	41.0
36	34.84829623053527	38.0	34.0	40.0	23.0	41.0
37	34.59187947333173	38.0	34.0	40.0	22.0	41.0
38	34.61615249761224	38.0	34.0	40.0	23.0	41.0
39	34.4856946207385	38.0	34.0	40.0	22.0	41.0
40	34.36147305076964	37.0	33.0	40.0	22.0	41.0
41	34.27591203944997	37.0	33.0	40.0	21.0	41.0
42	34.23603674896309	37.0	33.0	40.0	21.0	41.0
43	34.15686166936407	37.0	33.0	40.0	21.0	41.0
44	33.99469025862095	37.0	33.0	40.0	20.0	41.0
45	33.919897523626176	37.0	33.0	40.0	20.0	41.0
46	33.72161166984896	36.0	33.0	40.0	20.0	41.0
47	33.724654041103044	36.0	33.0	40.0	20.0	41.0
48	33.56237539924196	36.0	33.0	40.0	20.0	41.0
49	33.51356861558531	36.0	33.0	40.0	20.0	41.0
50	33.381912354770414	35.0	33.0	40.0	20.0	41.0
51	32.706891635889676	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	10.0
13	15.0
14	49.0
15	125.0
16	332.0
17	913.0
18	2007.0
19	3867.0
20	6610.0
21	10514.0
22	15769.0
23	23289.0
24	34545.0
25	52353.0
26	73696.0
27	84979.0
28	85292.0
29	86003.0
30	92008.0
31	106558.0
32	128206.0
33	161621.0
34	247528.0
35	341841.0
36	291378.0
37	366446.0
38	530368.0
39	862227.0
40	472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.06532591748413	23.12468152773587	26.68118027379679	19.12881228098321
2	34.47183963988078	24.51976668503727	27.351360906970218	13.657032768111737
3	27.588266865279753	25.478307588167116	33.85073040462152	13.082695141931607
4	24.25803409062886	27.7054175543713	33.49684000701574	14.539708347984096
5	22.95244673979995	30.14856912957426	32.38620824948109	14.512775881144696
6	20.830822268716748	38.905887930458825	29.581601910985984	10.681687889838452
7	84.75830189134079	3.2823528336030736	10.464537948870984	1.4948073261851462
8	85.39706131320159	2.5879274386143414	10.26808610741898	1.746925140765087
9	80.11004073951234	4.270652450092504	12.39140078475445	3.227906025640706
10	36.743421426329036	26.144719892646968	21.041488467667325	16.070370213356675
11	30.951666474093987	26.172649858258197	26.064171866821724	16.811511800826096
12	26.917005608436845	23.524678535239556	30.278493344605067	19.279822511718532
13	22.985281074372676	27.42847310369249	31.499016216836285	18.08722960509855
14	20.78740336384294	28.5384121537467	32.26204736068752	18.412137121722836
15	20.797212101765933	23.569288896979714	37.15120446591283	18.48229453534152
16	23.24066846826028	22.421999059580326	35.59155971955876	18.74577275260063
17	22.40972428302698	22.827011269242377	31.531905968007447	23.231358479723205
18	24.83943733310945	22.528842261362968	32.175403509034425	20.456316896493153
19	26.965190340776058	24.492778801599435	28.899589834046687	19.642441023577824
20	28.62555475478571	23.88557913254736	28.4686980729155	19.020168039751432
21	26.512215065168533	25.1424275850527	29.52671177023613	18.818645579542633
22	24.947749074750618	22.80035588539515	29.807313716411656	22.444581323442577
23	23.32312836673153	26.594508713844476	29.150820983051666	20.931541936372327
24	22.693429558714858	24.932010760795084	33.17595019377799	19.19860948671207
25	22.795091308543828	25.34159483982802	31.380507821083082	20.482806030545074
26	21.272076933755272	27.346733621000897	29.95890582143054	21.422283623813286
27	22.04408556655298	25.64696800550398	30.520525338269845	21.7884210896732
28	20.491312478404843	26.88117877754863	32.596708198636364	20.030800545410163
29	21.787756090491982	24.69524334398163	32.11624629020508	21.400754275321297
30	22.595203637988853	24.99274735267982	32.27257651439017	20.139472494941156
31	24.745949531549638	25.987447586288493	28.824971384253985	20.441631497907885
32	24.8865691500784	25.089005984161382	28.833754915105924	21.19066995065429
33	23.89616370284844	26.2357139472772	27.653603034834596	22.21451931503976
34	21.996122500607505	27.519910491110206	29.064814422280577	21.41915258600171
35	22.025908922266307	27.508660921627907	29.53610488367086	20.929325272434927
36	25.17725691716909	28.09383249280208	26.663308420801506	20.06560216922733
37	23.56743244093214	27.719022329287093	28.099956026929142	20.613589202851625
38	23.677462097124792	28.143014723913122	27.57128167785943	20.60824150110265
39	23.468402979528832	27.405142715751364	27.33775613205443	21.788698172665374
40	24.98911756548233	26.598249334238837	27.478763666772238	20.933869433506594
41	21.347083299737022	26.77569328242765	28.352129258107517	23.525094159727818
42	23.911763475307886	27.112293701321715	26.62354701142441	22.35239581194599
43	23.365660606030378	26.47771823264276	27.59394706661934	22.56267409470752
44	22.941557378207477	27.7222087836971	27.67804175474442	21.658192083350997
45	22.002162909836915	28.101895607874365	26.685391935277845	23.21054954701087
46	23.57580034729582	27.6800644605873	26.877355032256617	21.866780159860262
47	22.433802795046976	27.817469916406836	28.5141673919314	21.234559896614794
48	23.72858390918106	26.267717032873406	28.411535851629814	21.592163206315718
49	23.100353641022913	26.45596721775703	28.24764126175836	22.196037879461695
50	22.15652584477755	27.74387667368518	28.54508985385812	21.55450762767915
51	22.226350758805626	27.67543717461798	27.394862936741678	22.70334912983472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2159.0
1	3012.0
2	3865.0
3	32866.5
4	61868.0
5	44491.5
6	27115.0
7	27376.5
8	27638.0
9	28894.5
10	30151.0
11	30706.5
12	31262.0
13	30584.5
14	29907.0
15	28853.5
16	27800.0
17	26908.5
18	26017.0
19	24947.0
20	23877.0
21	22811.0
22	21745.0
23	22494.0
24	23243.0
25	24478.0
26	28619.0
27	31525.0
28	35996.0
29	40467.0
30	44423.0
31	48379.0
32	57030.5
33	65682.0
34	77498.0
35	89314.0
36	97590.0
37	105866.0
38	118539.5
39	131213.0
40	149659.5
41	168106.0
42	183495.0
43	198884.0
44	208411.5
45	217939.0
46	222062.5
47	226186.0
48	241229.5
49	256273.0
50	264864.5
51	273456.0
52	268707.0
53	263958.0
54	242916.0
55	221874.0
56	205325.5
57	188777.0
58	175390.5
59	162004.0
60	149557.5
61	137111.0
62	125147.0
63	113183.0
64	102654.0
65	92125.0
66	78389.5
67	64654.0
68	55699.0
69	46744.0
70	39508.5
71	32273.0
72	28846.0
73	25419.0
74	22718.0
75	16520.5
76	13024.0
77	9759.5
78	6495.0
79	4973.0
80	3451.0
81	2308.5
82	1166.0
83	885.0
84	604.0
85	442.0
86	280.0
87	207.5
88	135.0
89	94.0
90	53.0
91	34.0
92	15.0
93	14.0
94	13.0
95	6.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3609027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.846474976969017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22296724725264	18.65342031085877
2	5.812697193961466	2.7722467636900023
3	2.2661056717351427	1.6211589658859895
4	1.371447747339455	1.3081677755660337
5	1.0249667414587584	1.222092187621088
6	0.7444801472296002	1.0651936321056523
7	0.6480424004345476	1.0817468820184164
8	0.5637889895668734	1.0755504025597673
9	0.48288071955687834	1.0363502696196505
>10	7.37189758431384	42.663419313244574
>50	1.359019168481463	21.239759688778012
>100	0.12677099444651085	4.344143471338625
>500	0.003995319132729177	0.6608952470908278
>1k	8.225657037927781E-4	0.2795739614245837
>5k	0.0	0.0
>10k+	1.1750938625611116E-4	0.9762811281981051
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34840	0.9653571447373489	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7708299217489923E-5	0.0	0.0	0.029675588461931707	0.0
2	2.7708299217489923E-5	0.0	0.0	0.09625863148155998	0.0
3	2.7708299217489923E-5	0.0	0.0	0.1682447928485988	0.0
4	2.7708299217489923E-5	0.0	0.0	0.25993155495927295	0.0
5	2.7708299217489923E-5	0.0	0.0	0.42377072823229084	0.0
6	2.7708299217489923E-5	0.0	0.0	0.8763857959499888	0.0
7	2.7708299217489923E-5	0.0	0.0	1.1056442636755004	0.0
8	2.7708299217489923E-5	0.0	0.0	1.5916478319502736	0.0
9	5.541659843497985E-5	0.0	0.0	1.897076414224665	0.0
10	8.312489765246977E-5	0.0	0.0	2.317688396346162	0.0
11	8.312489765246977E-5	0.0	0.0	2.578645158376482	0.0
12	8.312489765246977E-5	0.0	0.0	2.804356963802155	0.0
13	8.312489765246977E-5	0.0	0.0	2.9483847031346677	0.0
14	8.312489765246977E-5	0.0	0.0	3.034502097102626	0.0
15	8.312489765246977E-5	0.0	0.0	3.101999513996432	0.0
16	1.108331968699597E-4	0.0	0.0	3.189945655712745	0.0
17	1.108331968699597E-4	0.0	0.0	3.2787507547048	0.0
18	1.108331968699597E-4	0.0	0.0	3.416904334603205	0.0
19	1.108331968699597E-4	0.0	0.0	3.494930905199656	0.0
20	1.385414960874496E-4	0.0	0.0	3.574758515245245	0.0
21	1.385414960874496E-4	0.0	0.0	3.658631536976587	0.0
22	1.6624979530493954E-4	0.0	0.0	3.7498749662997812	0.0
23	1.6624979530493954E-4	0.0	0.0	3.850428384160052	0.0
24	1.9395809452242945E-4	0.0	0.0	3.9429741035464683	0.0
25	1.9395809452242945E-4	0.0	0.0	4.027041083372333	0.0
26	1.9395809452242945E-4	0.0	0.0	4.105871194646092	0.0
27	1.9395809452242945E-4	0.0	0.0	4.1948425434334515	0.0
28	1.9395809452242945E-4	0.0	0.0	4.283259726236462	0.0
29	1.9395809452242945E-4	0.0	0.0	4.378493150646975	0.0
30	1.9395809452242945E-4	0.0	0.0	4.519888601553826	0.0
31	1.9395809452242945E-4	0.0	0.0	4.62404409831237	0.0
32	1.9395809452242945E-4	0.0	0.0	4.76424809235287	0.0
33	1.9395809452242945E-4	0.0	0.0	4.87175629331673	0.0
34	1.9395809452242945E-4	0.0	0.0	4.975219082594838	0.0
35	1.9395809452242945E-4	0.0	0.0	5.082339367369654	0.0
36	1.9395809452242945E-4	0.0	0.0	5.179096748237129	0.0
37	1.9395809452242945E-4	0.0	0.0	5.283446203090196	0.0
38	1.9395809452242945E-4	0.0	0.0	5.401428141158268	0.0
39	1.9395809452242945E-4	0.0	0.0	5.557730657044129	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28695	0.0	42.584946	1
AGGGCGA	5180	0.0	38.614864	6
TAGGGCG	2155	0.0	37.795822	5
TACGGGA	990	0.0	37.727272	4
GGGCGAT	10075	0.0	36.848633	7
AAGGGCG	2115	0.0	36.489365	5
AGCAACG	1795	0.0	36.476322	10
CACGACC	3655	0.0	36.381664	27
GTTTTTT	34520	0.0	36.3637	2
TATGGGC	1810	0.0	36.298344	4
GCGAGAC	3665	0.0	36.22101	21
ACGGGAT	890	0.0	36.151684	5
TATACGG	355	0.0	36.126762	2
AGACACG	3660	0.0	36.02459	24
TAGGGAC	2725	0.0	35.91743	5
ACACGAC	3690	0.0	35.73171	26
GACACGA	3690	0.0	35.73171	25
GACCGAT	2845	0.0	35.66784	9
TCAAGCG	3780	0.0	35.59524	17
CGAGACA	3750	0.0	35.52	22
>>END_MODULE