FastQCFastQC Report
Thu 26 May 2016
SRR1547652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547652_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1607981
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177131.1015677424049166No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG253.8915023E-545.01
CGTTTTT125550.043.3512541
CATACGA2300.041.0869618
TACGGGA3650.040.684934
TATTACG501.0822987E-940.51
TCGTAAG501.0822987E-940.51
GCGATTT2600.038.9423079
AGGGCGA19650.038.5877846
ATACCGG700.038.5714262
GTTTTTT144500.038.4446372
GGGCGAT39150.037.471267
GGCGATT9000.037.2500048
GACCGAT11450.037.139749
CGAGGGT3400.037.0588264
AGTACGG1950.036.9230772
CGAATAT1350.036.66666814
GGCGATA7550.036.655638
CGTAAGG2100.036.428572
AAGGGAC14700.036.428575
ACGGGTA2350.036.382985
Produced by FastQC (version 0.11.5)