##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547652_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1607981
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40602221046144	31.0	31.0	33.0	30.0	34.0
2	31.820748503869137	31.0	31.0	34.0	30.0	34.0
3	31.904121379543664	31.0	31.0	34.0	30.0	34.0
4	35.63950320308511	37.0	35.0	37.0	33.0	37.0
5	35.538078497196175	37.0	35.0	37.0	33.0	37.0
6	35.584744471483184	37.0	35.0	37.0	33.0	37.0
7	36.00508712478568	37.0	35.0	37.0	35.0	37.0
8	36.026370958363316	37.0	35.0	37.0	35.0	37.0
9	37.78213299784015	39.0	38.0	39.0	35.0	39.0
10	37.11459774711268	39.0	37.0	39.0	33.0	39.0
11	36.884804609009684	39.0	37.0	39.0	32.0	39.0
12	36.421113806692986	39.0	35.0	39.0	32.0	39.0
13	36.25671509800178	39.0	35.0	39.0	32.0	39.0
14	37.33840138658355	39.0	35.0	41.0	32.0	41.0
15	37.474854491439885	39.0	35.0	41.0	32.0	41.0
16	37.53241798255079	39.0	35.0	41.0	32.0	41.0
17	37.44026577428465	39.0	35.0	41.0	32.0	41.0
18	37.273612685722036	39.0	35.0	41.0	32.0	41.0
19	37.16232281351583	38.0	35.0	41.0	32.0	41.0
20	36.985217487022545	38.0	35.0	41.0	32.0	41.0
21	36.89068900689747	38.0	35.0	41.0	32.0	41.0
22	36.799691041125485	38.0	35.0	41.0	31.0	41.0
23	36.696534349597414	38.0	35.0	40.0	31.0	41.0
24	36.57832959469049	38.0	35.0	40.0	31.0	41.0
25	36.449086774035266	38.0	35.0	40.0	31.0	41.0
26	36.2682811550634	38.0	35.0	40.0	31.0	41.0
27	36.23556994765485	38.0	35.0	40.0	30.0	41.0
28	36.241717408352464	38.0	35.0	40.0	30.0	41.0
29	36.17763767109189	38.0	35.0	40.0	30.0	41.0
30	36.1151829530324	38.0	35.0	40.0	30.0	41.0
31	35.94567659692496	38.0	35.0	40.0	30.0	41.0
32	35.63382465340076	38.0	34.0	40.0	29.0	41.0
33	35.37892487535611	38.0	34.0	40.0	27.0	41.0
34	35.012492684926	38.0	34.0	40.0	24.0	41.0
35	34.80954563517852	38.0	34.0	40.0	23.0	41.0
36	34.618072601604126	38.0	34.0	40.0	23.0	41.0
37	34.37976505941302	37.0	33.0	40.0	22.0	41.0
38	34.43935842525502	37.0	34.0	40.0	23.0	41.0
39	34.320883766661424	37.0	33.0	40.0	22.0	41.0
40	34.1892043500514	37.0	33.0	40.0	21.0	41.0
41	34.12441689298568	37.0	33.0	40.0	21.0	41.0
42	34.077002153632414	37.0	33.0	40.0	21.0	41.0
43	33.991030988550236	37.0	33.0	40.0	21.0	41.0
44	33.82570689579043	36.0	33.0	40.0	20.0	41.0
45	33.76482993269199	36.0	33.0	40.0	20.0	41.0
46	33.57811193042704	36.0	33.0	40.0	20.0	41.0
47	33.59380676761728	36.0	33.0	40.0	20.0	41.0
48	33.40819325601484	35.0	33.0	40.0	20.0	41.0
49	33.388706707355375	35.0	33.0	40.0	20.0	41.0
50	33.2519924053829	35.0	33.0	40.0	19.0	41.0
51	32.58117539946056	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	2.0
13	13.0
14	22.0
15	54.0
16	125.0
17	366.0
18	792.0
19	1725.0
20	3001.0
21	4800.0
22	7398.0
23	10926.0
24	16446.0
25	25331.0
26	34926.0
27	38183.0
28	37095.0
29	37665.0
30	41864.0
31	49707.0
32	60467.0
33	76403.0
34	121733.0
35	172101.0
36	129755.0
37	156474.0
38	221821.0
39	358569.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.110504415164108	23.454754751455397	27.360273535570386	18.074467297810113
2	34.89406902195984	24.770566318880633	27.379365800964067	12.955998858195464
3	26.43059837149817	25.2175243364194	34.893136175116496	13.458741116965934
4	23.33790013687973	28.457612372285496	34.14088848064747	14.063599010187309
5	21.51580149267933	31.731034135353593	33.41382765094861	13.33933672101847
6	19.656762113482685	40.04705279477805	30.124734060912413	10.171451030826857
7	84.30335930586244	3.5142828180183723	10.605224812979754	1.5771330631394278
8	85.14578219518762	2.8025206765502824	10.00639932934531	2.045297798916778
9	79.83657767100482	4.702543127064312	12.319672931458767	3.1412062704721015
10	41.164665502888404	22.9413158488813	21.015484635701544	14.878534012528755
11	34.791144920244705	25.21920346073741	23.899971454886593	16.08968016413129
12	28.77577533565384	22.66656135862302	30.004894336438053	18.552768969285086
13	22.647717852387558	29.17671290892119	31.31722327564816	16.858345963043096
14	20.134628456430768	30.801545540650043	31.948076500903927	17.11574950201526
15	19.17199270389389	24.366457066345934	39.22583662369145	17.235713606068728
16	21.176183051914172	22.42234205503672	37.654984729297176	18.746490163751936
17	21.6894353851196	23.49978015909392	30.876048908538102	23.93473554724838
18	24.504580588949747	23.976216136882215	32.22140062600242	19.297802648165618
19	25.968776994255528	27.61008992021672	27.295409585063506	19.125723500464247
20	27.66201839449596	25.62698191085591	27.667677665345547	19.043322029302583
21	24.471060292379075	27.406045220683577	28.863400749138208	19.25949373779914
22	24.112038637272455	24.225535003212105	29.35942651063663	22.30299984887881
23	22.104490040616152	27.865627765502204	28.00232092294623	22.027561270935415
24	22.653625882395374	25.364976327456606	33.06755490270096	18.913842887447053
25	21.88806957296137	26.425685378123248	30.83637182280139	20.849873226113992
26	20.747260073346638	29.155133051945263	29.251340656388354	20.846266218319744
27	20.60273100241856	27.282474108835864	30.29500970471666	21.819785184028916
28	19.571562101791002	27.519728155991892	32.93633444673787	19.97237529547924
29	21.021143906551135	26.082522119353403	31.523133668867974	21.37320030522749
30	21.836016719103025	27.32725075731616	31.16187318133734	19.674859342243472
31	24.775292742886887	26.615115477110734	27.699954166125096	20.909637613877276
32	24.7967482202837	26.85678499932524	27.56836057142466	20.7781062089664
33	23.781499905782468	27.87327710961759	26.751746444765207	21.593476539834736
34	20.62785567739917	28.584106404242338	28.23690080915135	22.551137109207136
35	21.710455533989517	28.34772301414009	28.621669037134147	21.320152414736242
36	24.34058611389065	28.217186645862107	26.385697343438757	21.05652989680848
37	22.34193065714085	29.24275846542963	27.951885003616333	20.463425873813186
38	22.646536246385995	29.168441666910244	26.765614767836187	21.419407318867574
39	22.109092085043294	29.053079607283916	26.80112513767265	22.036703170000145
40	23.475774900325312	27.28421542294343	26.847145581944066	22.39286409478719
41	20.471510546455463	26.514243638450953	27.929185730428408	25.08506008466518
42	22.889698323549844	27.91736967041277	26.07145233681244	23.121479669224946
43	22.378436063610206	26.912755809925613	28.12427510026549	22.58453302619869
44	22.078805657529536	28.905005718351152	27.11953686019922	21.896651763920097
45	21.033333105304106	30.70931808273854	25.47418159791689	22.783167214040464
46	23.04461308933377	29.14487173666853	25.966786920989737	21.843728253007967
47	22.60275463453859	28.74741679161632	27.058528676644812	21.59129989720028
48	22.93746008192883	26.803426159886218	27.815254035961868	22.443859722223085
49	22.55126148878625	25.643648774456913	28.431057332145095	23.374032404611746
50	21.278112116996407	28.30543395724203	28.1707308730638	22.24572305269776
51	20.75248401566934	28.96147404726797	27.129549416317726	23.15649252074496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1029.0
1	1264.0
2	1499.0
3	14022.5
4	26546.0
5	18198.0
6	9850.0
7	10311.5
8	10773.0
9	11932.0
10	13091.0
11	13922.5
12	14754.0
13	15024.0
14	15294.0
15	14977.0
16	14660.0
17	14050.0
18	13440.0
19	12912.0
20	12384.0
21	12081.0
22	11778.0
23	11309.5
24	10841.0
25	11206.5
26	12659.0
27	13746.0
28	14909.5
29	16073.0
30	18292.0
31	20511.0
32	23398.0
33	26285.0
34	32533.5
35	38782.0
36	42570.5
37	46359.0
38	51743.5
39	57128.0
40	67417.5
41	77707.0
42	88563.0
43	99419.0
44	106401.0
45	113383.0
46	119262.5
47	125142.0
48	126385.5
49	127629.0
50	123270.5
51	118912.0
52	110684.0
53	102456.0
54	95177.5
55	87899.0
56	83006.0
57	78113.0
58	73589.0
59	69065.0
60	63682.0
61	58299.0
62	53124.5
63	47950.0
64	42729.5
65	37509.0
66	31981.5
67	26454.0
68	22098.0
69	17742.0
70	14945.0
71	12148.0
72	10289.0
73	8430.0
74	7094.0
75	4639.5
76	3521.0
77	2906.0
78	2291.0
79	1647.5
80	1004.0
81	685.0
82	366.0
83	248.5
84	131.0
85	157.0
86	183.0
87	110.5
88	38.0
89	29.0
90	20.0
91	14.0
92	8.0
93	7.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1607981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.235777193604218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.86445422524484	22.93820734868889
2	6.8758923412087825	4.157958976719969
3	2.8929726852815123	2.624138326180641
4	1.8359721320165576	2.220481772692766
5	1.3235046647823387	2.000859607952732
6	1.0690742530971828	1.9394574552039163
7	0.8822763229124108	1.867341722594044
8	0.7894604782569844	1.909596089898753
9	0.677723074797872	1.8442335499697542
>10	7.333223102635245	47.288980055716436
>50	0.4233462004695108	8.110630489042686
>100	0.029599183291513276	1.4423883738117511
>500	0.002084446671072031	0.4522034072251224
>1k	2.0844466710720312E-4	0.08716338845886332
>5k	0.0	0.0
>10k+	2.0844466710720312E-4	1.1163594358437063
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17713	1.1015677424049166	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043781611847403666	0.0
2	0.0	0.0	0.0	0.1407976835547186	0.0
3	0.0	0.0	0.0	0.24795069095965686	0.0
4	0.0	0.0	0.0	0.38731800935458816	0.0
5	0.0	0.0	0.0	0.6491370233852266	0.0
6	0.0	0.0	0.0	1.3880761028892754	0.0
7	0.0	0.0	0.0	1.7974714875362334	0.0
8	0.0	0.0	0.0	2.6258394844217685	0.0
9	0.0	0.0	0.0	3.1466167821634707	0.0
10	0.0	0.0	0.0	3.7822586212150515	0.0
11	0.0	0.0	0.0	4.17791006237014	0.0
12	0.0	0.0	0.0	4.5414715721143475	0.0
13	0.0	0.0	0.0	4.782270437275067	0.0
14	0.0	0.0	0.0	4.926115420518029	0.0
15	0.0	0.0	0.0	5.033454997291635	0.0
16	0.0	0.0	0.0	5.169775015998323	0.0
17	0.0	0.0	0.0	5.3136199992412845	0.0
18	0.0	0.0	0.0	5.5460854326015046	0.0
19	0.0	0.0	0.0	5.663997273599626	0.0
20	0.0	0.0	0.0	5.798886927146527	0.0
21	0.0	0.0	0.0	5.935828843748776	0.0
22	0.0	0.0	0.0	6.09012171163714	0.0
23	0.0	0.0	0.0	6.262263049128068	0.0
24	0.0	0.0	0.0	6.420162924810679	0.0
25	0.0	0.0	0.0	6.553373454039569	0.0
26	0.0	0.0	0.0	6.686459603689347	0.0
27	0.0	0.0	0.0	6.820043271655573	0.0
28	0.0	0.0	0.0	6.961338473526739	0.0
29	0.0	0.0	0.0	7.110096450144622	0.0
30	0.0	0.0	0.0	7.319489471579577	0.0
31	0.0	0.0	0.0	7.471419127464815	0.0
32	0.0	0.0	0.0	7.656806890131165	0.0
33	0.0	0.0	0.0	7.817194357396014	0.0
34	0.0	0.0	0.0	7.981126642665554	0.0
35	0.0	0.0	0.0	8.141451920140847	0.0
36	0.0	0.0	0.0	8.297548291926335	0.0
37	0.0	0.0	0.0	8.460734299721203	0.0
38	0.0	0.0	0.0	8.652900749449154	0.0
39	0.0	0.0	0.0	8.903090272832825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	25	3.8915023E-5	45.0	1
CGTTTTT	12555	0.0	43.351254	1
CATACGA	230	0.0	41.08696	18
TACGGGA	365	0.0	40.68493	4
TATTACG	50	1.0822987E-9	40.5	1
TCGTAAG	50	1.0822987E-9	40.5	1
GCGATTT	260	0.0	38.942307	9
AGGGCGA	1965	0.0	38.587784	6
ATACCGG	70	0.0	38.571426	2
GTTTTTT	14450	0.0	38.444637	2
GGGCGAT	3915	0.0	37.47126	7
GGCGATT	900	0.0	37.250004	8
GACCGAT	1145	0.0	37.13974	9
CGAGGGT	340	0.0	37.058826	4
AGTACGG	195	0.0	36.923077	2
CGAATAT	135	0.0	36.666668	14
GGCGATA	755	0.0	36.65563	8
CGTAAGG	210	0.0	36.42857	2
AAGGGAC	1470	0.0	36.42857	5
ACGGGTA	235	0.0	36.38298	5
>>END_MODULE