FastQCFastQC Report
Thu 26 May 2016
SRR1547651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547651_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3825938
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237200.6199786823518834No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44790.11706933044916044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT187300.040.807531
GGGCGAT103900.037.2473537
AGGGCGA51650.037.0716366
CACGACC43900.037.00455527
ACGACCA44550.036.66666428
AGACACG43850.036.63625724
CGACCAA44600.036.5751129
TACGGGA9050.036.546964
GACACGA44250.036.40677625
AACACGT40400.036.36757741
ACACGAC44300.036.3656926
AAACACG44700.036.04026840
GCGATAA3750.036.09
TCAAGCG44750.036.017
CGTTCGG5150.035.8252445
TGGTCAA45250.035.80110514
GACCGAT30750.035.5609789
ACGTGAG41500.035.18674544
ACACGTG41700.035.17985542
GCGAGAC45800.035.1255521
Produced by FastQC (version 0.11.5)