##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547651_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3825938
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.421771863527322	31.0	31.0	33.0	30.0	34.0
2	31.83310236600802	31.0	31.0	34.0	30.0	34.0
3	31.950280689336836	31.0	31.0	34.0	30.0	34.0
4	35.655601841953526	37.0	35.0	37.0	33.0	37.0
5	35.54758048875858	37.0	35.0	37.0	33.0	37.0
6	35.58760701297303	37.0	35.0	37.0	33.0	37.0
7	35.980189956031694	37.0	35.0	37.0	35.0	37.0
8	35.98375378795997	37.0	35.0	37.0	35.0	37.0
9	37.714323389453774	39.0	37.0	39.0	35.0	39.0
10	37.129463676619956	39.0	37.0	39.0	33.0	39.0
11	36.889153718643634	39.0	37.0	39.0	32.0	39.0
12	36.41514002579237	39.0	35.0	39.0	32.0	39.0
13	36.249831544578086	39.0	35.0	39.0	32.0	39.0
14	37.3083863355862	39.0	35.0	41.0	32.0	41.0
15	37.439847169504574	39.0	35.0	41.0	32.0	41.0
16	37.496228375891086	39.0	35.0	41.0	32.0	41.0
17	37.387894419616835	39.0	35.0	41.0	32.0	41.0
18	37.281632896298895	39.0	35.0	41.0	32.0	41.0
19	37.21704716594989	39.0	35.0	41.0	32.0	41.0
20	37.099555455420344	39.0	35.0	41.0	32.0	41.0
21	36.96265621659316	39.0	35.0	41.0	31.0	41.0
22	36.86800883861683	38.0	35.0	41.0	31.0	41.0
23	36.74299139191487	38.0	35.0	41.0	31.0	41.0
24	36.60886271549617	38.0	35.0	41.0	31.0	41.0
25	36.46663380326602	38.0	35.0	40.0	31.0	41.0
26	36.29803645537382	38.0	35.0	40.0	30.0	41.0
27	36.277640672692556	38.0	35.0	40.0	30.0	41.0
28	36.28325262981261	38.0	35.0	40.0	30.0	41.0
29	36.235390902832194	38.0	35.0	40.0	30.0	41.0
30	36.25022778727726	38.0	35.0	40.0	30.0	41.0
31	36.1057259683769	38.0	35.0	40.0	30.0	41.0
32	35.873630205194125	38.0	35.0	40.0	29.0	41.0
33	35.68778323119716	38.0	35.0	40.0	29.0	41.0
34	35.42458450711956	38.0	34.0	40.0	27.0	41.0
35	35.262118988859726	38.0	34.0	40.0	26.0	41.0
36	35.0748496185772	38.0	34.0	40.0	25.0	41.0
37	34.81322802408194	37.0	34.0	40.0	24.0	41.0
38	34.83360341960586	37.0	34.0	40.0	24.0	41.0
39	34.716287613651865	37.0	34.0	40.0	24.0	41.0
40	34.51980795297781	37.0	33.0	40.0	23.0	41.0
41	34.44345857146666	37.0	33.0	40.0	23.0	41.0
42	34.391195309490115	37.0	33.0	40.0	23.0	41.0
43	34.30178612408251	36.0	33.0	40.0	23.0	41.0
44	34.14167270875796	36.0	33.0	40.0	23.0	41.0
45	34.060325337211424	36.0	33.0	40.0	23.0	41.0
46	33.861796244476515	35.0	33.0	40.0	23.0	41.0
47	33.87042367126702	35.0	33.0	40.0	23.0	41.0
48	33.70088067292256	35.0	33.0	40.0	22.0	41.0
49	33.65654331042479	35.0	33.0	40.0	22.0	41.0
50	33.49283548243594	35.0	33.0	39.0	22.0	41.0
51	32.79511978500436	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	4.0
12	11.0
13	20.0
14	59.0
15	156.0
16	444.0
17	1098.0
18	2378.0
19	4491.0
20	7711.0
21	11691.0
22	17280.0
23	24893.0
24	35415.0
25	50395.0
26	65466.0
27	76157.0
28	81277.0
29	88305.0
30	100666.0
31	118542.0
32	143830.0
33	182812.0
34	290531.0
35	416647.0
36	312967.0
37	394431.0
38	546560.0
39	851205.0
40	492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.473951224510174	23.773621004835938	26.6013981407958	15.15102962985809
2	33.815968789875846	25.034383724984565	26.999470456656642	14.150177028482949
3	29.21790682441796	26.10795052089187	30.834033379526797	13.840109275163373
4	25.342830960668994	28.53391769547755	30.59921514671696	15.524036197136493
5	23.501138805699412	31.348364767019227	29.54224558787936	15.608250839401997
6	21.638066272898307	39.929293156344926	27.469028510132677	10.963612060624088
7	85.98714877240562	4.446491291808702	7.821820426781616	1.7445395090040665
8	86.18579286961786	3.584506596813644	7.778850572068863	2.4508499614996375
9	81.0037433957372	5.374499011745616	10.145119967966025	3.476637624551156
10	43.09761423211772	27.08201230652457	15.979323240470702	13.841050220887007
11	35.5605082988799	26.679705734907362	22.465340525643647	15.294445440569085
12	29.622591897725474	24.192995286384672	27.650082149789153	18.534330666100704
13	23.717582459517118	29.874869901185015	29.05250947610756	17.355038163190308
14	20.84994581720875	30.93657032602201	30.36481511200652	17.848668744762723
15	20.45806806069518	23.538593672976404	37.526954174375014	18.4763840919534
16	23.048831423823387	22.165649312665288	35.432801054277405	19.352718209233917
17	22.209795349532584	22.755909792578972	29.206955261690076	25.827339596198367
18	26.145431525550073	22.005479440597313	30.220876553671282	21.62821248018133
19	28.677673292144306	25.02246507915183	25.457260415615725	20.842601213088134
20	30.86108556908136	24.142236492070705	24.852859612466276	20.14381832638166
21	27.078849683397898	26.360097837445352	26.573509555042452	19.987542924114297
22	26.651843286535225	23.21613680096227	25.994906347149378	24.137113565353125
23	24.145765038534343	27.929908953046283	25.571506908893976	22.352819099525398
24	23.638203232775858	24.966740182407555	31.422621067042904	19.972435517773683
25	23.21428104689621	25.458802521107245	29.587463257376363	21.739453174620184
26	21.919748830221504	28.451323570847205	27.206321691569492	22.422605907361802
27	23.126485583404644	26.18829683073798	27.844727227675932	22.840490358181444
28	21.399902455293315	27.237320625687083	30.930401904055948	20.43237501496365
29	22.075553759627052	25.21841702609922	30.48886312323932	22.217166091034407
30	23.68778584493528	25.45616264560482	29.580615263498782	21.27543624596112
31	26.847873645626247	25.789597217728044	25.96970991166088	21.392819224984827
32	27.311341689279857	25.06637587958822	26.53610696252788	21.08617546860404
33	25.751358229014688	25.603525200878842	25.6542578578116	22.990858712294866
34	22.603424310587364	27.80944176303955	28.50197781563632	21.085156110736765
35	23.438801151508468	27.501621824504213	28.02157274895725	21.03800427503007
36	27.092728632821544	27.872458989142007	24.84371152904203	20.191100848994417
37	24.554030933067917	28.341755668805924	25.951073958856625	21.153139439269534
38	24.699877520231638	29.247337515662824	25.05464019542397	20.998144768681563
39	24.942693791692392	28.13375438911974	24.51613173031032	22.40742008887755
40	26.81449620981835	26.32815795760412	25.148159745401	21.70918608717653
41	22.30357627332173	26.07831073059731	25.96077092728633	25.65734206879463
42	25.471348464089065	26.171621181524635	24.9618786295021	23.395151724884197
43	24.582494541207932	25.568213598861245	26.259129133822867	23.59016272610795
44	24.030446912626395	27.532699170765447	26.642329279774007	21.794524636834154
45	22.367011697523587	28.32505388221137	25.1276941759119	24.18024024435315
46	23.939933161488764	27.69156217377281	25.681937344515255	22.686567320223173
47	23.406207837136932	27.221533647434953	27.400417884450817	21.971840630977294
48	25.050719588242153	25.306264764353216	27.50682316336543	22.136192484039206
49	24.282803328229573	25.451614741273904	27.201303314376762	23.06427861611976
50	22.577522165806137	28.491261489339347	26.882688637400815	22.0485277074537
51	22.912551118183305	28.29387198642529	25.365178421605368	23.428398473786036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2665.0
1	3554.0
2	4443.0
3	22341.5
4	40240.0
5	28838.0
6	17436.0
7	18523.0
8	19610.0
9	20806.5
10	22003.0
11	22797.0
12	23591.0
13	23883.5
14	24176.0
15	23574.0
16	22972.0
17	22355.5
18	21739.0
19	22118.0
20	22497.0
21	22578.5
22	22660.0
23	23246.0
24	23832.0
25	24062.5
26	27830.0
27	31367.0
28	34498.5
29	37630.0
30	42296.5
31	46963.0
32	55641.5
33	64320.0
34	73868.0
35	83416.0
36	92235.0
37	101054.0
38	113774.0
39	126494.0
40	146257.5
41	166021.0
42	195472.5
43	224924.0
44	234678.5
45	244433.0
46	254724.5
47	265016.0
48	270246.0
49	275476.0
50	273620.5
51	271765.0
52	266548.0
53	261331.0
54	245454.5
55	229578.0
56	213593.0
57	197608.0
58	189636.5
59	181665.0
60	173112.5
61	164560.0
62	150710.5
63	136861.0
64	125506.5
65	114152.0
66	101549.0
67	88946.0
68	77765.5
69	66585.0
70	57127.5
71	47670.0
72	43458.5
73	39247.0
74	34697.5
75	24274.0
76	18400.0
77	14266.0
78	10132.0
79	7314.5
80	4497.0
81	3251.5
82	2006.0
83	1411.5
84	817.0
85	639.0
86	461.0
87	291.5
88	122.0
89	89.0
90	56.0
91	47.0
92	38.0
93	26.0
94	14.0
95	9.5
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3825938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.84803668644208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07309075482473	16.90214770339834
2	5.974687940019355	2.491210267271331
3	1.989010407661106	1.2440088584590157
4	1.092067261510297	0.9106983332811603
5	0.7637726219078335	0.7961579820817282
6	0.551832526055607	0.6902774848787578
7	0.3882572428556287	0.5666080869981699
8	0.33753012568643526	0.5629472354472166
9	0.30851772428513474	0.57887899508827
>10	4.819529242494747	25.799411697046803
>50	2.0934683920102697	30.946400876529705
>100	0.6019977745857766	16.68047030387555
>500	0.0044557043594358504	0.6233364769088503
>1k	0.0016549759049332947	0.5778986821175667
>5k	0.0	0.0
>10k+	1.2730583884102266E-4	0.629547016617465
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23720	0.6199786823518834	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4479	0.11706933044916044	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5682428727282043E-4	0.0	0.0	0.02017805829576956	0.0
2	1.5682428727282043E-4	0.0	0.0	0.06244220371579466	0.0
3	1.5682428727282043E-4	0.0	0.0	0.11492606518976523	0.0
4	1.5682428727282043E-4	0.0	0.0	0.22130520672316176	0.0
5	1.5682428727282043E-4	0.0	0.0	0.37413047467052524	0.0
6	1.5682428727282043E-4	0.0	0.0	0.8783989703962793	0.0
7	1.5682428727282043E-4	0.0	0.0	1.1401125684734044	0.0
8	1.5682428727282043E-4	0.0	0.0	1.8261404131483574	0.0
9	1.5682428727282043E-4	0.0	0.0	2.233360812433448	0.0
10	2.0909904969709388E-4	0.0	0.0	2.7680270825089166	0.0
11	2.0909904969709388E-4	0.0	0.0	3.077153890104858	0.0
12	2.0909904969709388E-4	0.0	0.0	3.3498190509098684	0.0
13	2.0909904969709388E-4	0.0	0.0	3.5143800030214813	0.0
14	2.0909904969709388E-4	0.0	0.0	3.6011299712645632	0.0
15	2.0909904969709388E-4	0.0	0.0	3.6640949226046007	0.0
16	2.0909904969709388E-4	0.0	0.0	3.7523869963391983	0.0
17	2.0909904969709388E-4	0.0	0.0	3.8393983383944015	0.0
18	2.0909904969709388E-4	0.0	0.0	3.976201391658725	2.6137381212136735E-5
19	2.0909904969709388E-4	0.0	0.0	4.054456711007862	2.6137381212136735E-5
20	2.6137381212136735E-4	0.0	0.0	4.132921129406697	2.6137381212136735E-5
21	2.6137381212136735E-4	0.0	0.0	4.2193574490752335	2.6137381212136735E-5
22	2.875111933335041E-4	0.0	0.0	4.30898252925165	2.6137381212136735E-5
23	2.875111933335041E-4	0.0	0.0	4.415492357691107	2.6137381212136735E-5
24	2.875111933335041E-4	0.0	0.0	4.503000309989341	2.6137381212136735E-5
25	2.875111933335041E-4	0.0	0.0	4.580288546233629	2.6137381212136735E-5
26	2.875111933335041E-4	0.0	0.0	4.652453855760339	2.6137381212136735E-5
27	2.875111933335041E-4	0.0	0.0	4.733375187993114	2.6137381212136735E-5
28	2.875111933335041E-4	0.0	0.0	4.812728277353162	2.6137381212136735E-5
29	2.875111933335041E-4	0.0	0.0	4.905829629230793	2.6137381212136735E-5
30	2.875111933335041E-4	0.0	0.0	5.0387120753132955	2.6137381212136735E-5
31	2.875111933335041E-4	0.0	0.0	5.13858300892487	2.6137381212136735E-5
32	2.875111933335041E-4	0.0	0.0	5.271465455007373	2.6137381212136735E-5
33	2.875111933335041E-4	0.0	0.0	5.370160206464401	2.6137381212136735E-5
34	2.875111933335041E-4	0.0	0.0	5.468358347678399	2.6137381212136735E-5
35	2.875111933335041E-4	0.0	0.0	5.578997882349374	2.6137381212136735E-5
36	2.875111933335041E-4	0.0	0.0	5.6752879947348855	2.6137381212136735E-5
37	2.875111933335041E-4	0.0	0.0	5.778765886953735	2.6137381212136735E-5
38	2.875111933335041E-4	0.0	0.0	5.89612272859623	2.6137381212136735E-5
39	2.875111933335041E-4	0.0	0.0	6.087631320737555	2.6137381212136735E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18730	0.0	40.80753	1
GGGCGAT	10390	0.0	37.247353	7
AGGGCGA	5165	0.0	37.071636	6
CACGACC	4390	0.0	37.004555	27
ACGACCA	4455	0.0	36.666664	28
AGACACG	4385	0.0	36.636257	24
CGACCAA	4460	0.0	36.57511	29
TACGGGA	905	0.0	36.54696	4
GACACGA	4425	0.0	36.406776	25
AACACGT	4040	0.0	36.367577	41
ACACGAC	4430	0.0	36.36569	26
AAACACG	4470	0.0	36.040268	40
GCGATAA	375	0.0	36.0	9
TCAAGCG	4475	0.0	36.0	17
CGTTCGG	515	0.0	35.82524	45
TGGTCAA	4525	0.0	35.801105	14
GACCGAT	3075	0.0	35.560978	9
ACGTGAG	4150	0.0	35.186745	44
ACACGTG	4170	0.0	35.179855	42
GCGAGAC	4580	0.0	35.12555	21
>>END_MODULE