FastQCFastQC Report
Thu 26 May 2016
SRR1547650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547650_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3057021
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277080.9063725764396122No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32120.10506960861570791No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC253.8923314E-545.029
CGTTTTT182450.043.2118381
AGGGCGA40350.038.9219366
GGGCGAT80500.038.2919247
CGTAAGG4650.038.2258032
ACGGGTA7400.038.0067565
CGGGTAT5000.037.8000036
TACGGGT6400.037.6171884
TAGGGTA24200.037.5619855
TAGGGTC22200.037.0945975
TGGGCGA21900.037.089046
CGTTAGG5400.037.0833362
GTTTTTT217100.036.9368972
TAAGGGT26900.036.886624
TAGGGCG18050.036.772855
ATAGGGT28800.036.6406254
CACGACC18700.036.57753827
AGGGTAC21700.036.4976966
TAGGGAC23800.036.397065
TATACTA17850.036.30252544
Produced by FastQC (version 0.11.5)