##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547650_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3057021
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41031121474141	31.0	31.0	33.0	30.0	34.0
2	31.839856513906838	31.0	31.0	34.0	30.0	34.0
3	31.953441602134887	31.0	31.0	34.0	30.0	34.0
4	35.66461434187073	37.0	35.0	37.0	33.0	37.0
5	35.5566046160625	37.0	35.0	37.0	33.0	37.0
6	35.6047433105628	37.0	35.0	37.0	33.0	37.0
7	36.02985717140968	37.0	35.0	37.0	35.0	37.0
8	36.052400032580735	37.0	35.0	37.0	35.0	37.0
9	37.78407999159967	39.0	38.0	39.0	35.0	39.0
10	37.02788237306842	39.0	37.0	39.0	33.0	39.0
11	36.90604578771294	39.0	37.0	39.0	33.0	39.0
12	36.54948461263432	39.0	35.0	39.0	32.0	39.0
13	36.39398846131577	39.0	35.0	39.0	32.0	39.0
14	37.490199772916185	40.0	36.0	41.0	32.0	41.0
15	37.618989532620155	40.0	36.0	41.0	32.0	41.0
16	37.66725907345746	40.0	36.0	41.0	33.0	41.0
17	37.57829795739055	39.0	35.0	41.0	32.0	41.0
18	37.451072465645474	39.0	36.0	41.0	32.0	41.0
19	37.37560226115555	39.0	36.0	41.0	32.0	41.0
20	37.24639313894147	39.0	35.0	41.0	32.0	41.0
21	37.15277683731973	39.0	35.0	41.0	32.0	41.0
22	37.049593705767805	39.0	35.0	41.0	32.0	41.0
23	36.93190854756968	39.0	35.0	41.0	32.0	41.0
24	36.827216103520385	38.0	35.0	41.0	31.0	41.0
25	36.70656367751481	38.0	35.0	41.0	31.0	41.0
26	36.571533201767345	38.0	35.0	40.0	31.0	41.0
27	36.54785753843366	38.0	35.0	41.0	31.0	41.0
28	36.58074609235592	38.0	35.0	41.0	31.0	41.0
29	36.55878549738455	38.0	35.0	41.0	31.0	41.0
30	36.5554135872799	38.0	35.0	41.0	31.0	41.0
31	36.40202635179804	38.0	35.0	41.0	30.0	41.0
32	36.12220001105651	38.0	35.0	41.0	30.0	41.0
33	35.93317808415448	38.0	35.0	41.0	29.0	41.0
34	35.65670795195715	38.0	35.0	41.0	27.0	41.0
35	35.489078419808045	38.0	35.0	41.0	27.0	41.0
36	35.31224777324068	38.0	35.0	40.0	26.0	41.0
37	35.0798434161885	38.0	34.0	40.0	24.0	41.0
38	35.14625283895662	38.0	34.0	40.0	25.0	41.0
39	35.0483572078831	38.0	34.0	40.0	24.0	41.0
40	34.925201364334754	38.0	34.0	40.0	24.0	41.0
41	34.875906642447006	38.0	34.0	40.0	24.0	41.0
42	34.825765344758835	38.0	34.0	40.0	23.0	41.0
43	34.74644204275993	38.0	34.0	40.0	23.0	41.0
44	34.582727433014036	37.0	34.0	40.0	23.0	41.0
45	34.52816025797664	37.0	34.0	40.0	23.0	41.0
46	34.34849057301209	37.0	34.0	40.0	23.0	41.0
47	34.37061505302057	37.0	33.0	40.0	23.0	41.0
48	34.2062743435521	37.0	33.0	40.0	23.0	41.0
49	34.191457631465404	36.0	33.0	40.0	23.0	41.0
50	34.0462597411009	36.0	33.0	40.0	23.0	41.0
51	33.3722787641956	35.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	11.0
14	24.0
15	92.0
16	237.0
17	654.0
18	1497.0
19	2903.0
20	4866.0
21	7822.0
22	11959.0
23	17558.0
24	26125.0
25	38739.0
26	52602.0
27	59912.0
28	61926.0
29	65570.0
30	73710.0
31	87351.0
32	108127.0
33	136442.0
34	214340.0
35	321931.0
36	237436.0
37	295598.0
38	440656.0
39	788490.0
40	436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.630316572898913	24.445334199536084	28.969640705772058	16.95470852179295
2	32.50213197750359	25.161521625137674	28.638926588989737	13.697419808368997
3	27.020128419137457	25.252100001929982	34.01177159070873	13.715999988223828
4	23.77821415031169	28.72871988776001	33.09319759334332	14.399868368584972
5	22.551006355533705	31.823432027454178	31.80985671998982	13.815704897022297
6	20.507808091602904	40.64182090996431	28.107461479656177	10.742909518776612
7	86.82400284459936	3.3718119698883324	8.377567573137377	1.4266176123749232
8	87.63037610798224	2.419087078564393	7.910446150026447	2.0400906634269114
9	81.40964684246526	4.090092936882018	11.277972902377838	3.222287318274883
10	36.0977893184247	22.119573270841123	27.498633473567896	14.28400393716628
11	32.91541013293661	24.27958460213391	25.461944814903138	17.34306045002635
12	29.024399897809012	24.04693981493748	28.1337288818101	18.794931405443403
13	22.10501661584922	30.546273643524202	30.302670475603538	17.04603926502304
14	19.790573895305265	31.91499175177403	30.53750693894481	17.75692741397589
15	18.933137848905844	24.52115965183098	39.45880646551005	17.08689603375312
16	21.237799805758613	21.908845245093183	37.913576648639314	18.939778300508895
17	20.741663207416632	23.23657573827592	30.696288968901424	25.32547208540602
18	23.17897063840909	23.169026316796646	32.78011502047254	20.871888024321716
19	25.52798950350684	25.79318231703348	28.749295474254183	19.929532705205492
20	27.701608853848242	24.9332929018152	27.573477578335247	19.791620666001315
21	23.912527915248212	27.085060913876614	29.351875567750437	19.65053560312474
22	23.631633541280873	23.640989054376792	29.36378258441797	23.363594819924362
23	21.155693729287435	28.037164285099774	28.236737660617965	22.570404324994822
24	20.920497438519394	26.07891800547003	33.94674096121682	19.053843594793754
25	20.63947221821505	26.743192146864548	31.81724953803065	20.80008609688975
26	20.047163562173765	29.69518364446957	29.185569873415986	21.072082919940687
27	20.273135186182888	28.27523919528194	29.975620056257384	21.476005562277788
28	18.76673401981864	27.88865369259812	33.94772230874436	19.396889978838878
29	19.811018635462432	25.407316469203188	33.38413442367586	21.39753047165852
30	21.86717068675681	26.58012489937099	32.205241638837286	19.347462775034913
31	25.255436583523633	26.47080278480259	28.365294186726224	19.90846644494755
32	25.692528772291716	26.057066667190053	28.49587883105808	19.754525729460152
33	24.021261221300083	27.782766294376128	27.477338232220188	20.7186342521036
34	20.845522487415035	27.36955356211161	30.3721171689694	21.412806781503953
35	20.961190649328216	27.790028266079954	30.426058571400066	20.822722513191763
36	24.16960825588048	27.7029173172183	28.66156300529175	19.46591142160947
37	21.22363568977773	29.349422198931574	29.593614175368764	19.833327935921933
38	21.277609803792647	29.24755832557251	28.572783765633275	20.90204810500157
39	20.80057677065352	29.662472060218104	28.165230137444265	21.371721031684114
40	22.846130268650427	27.334813859636554	28.012826866416685	21.80622900529633
41	19.61962315600711	26.81885404123819	28.63784710670944	24.923675696045265
42	22.600302713000666	27.641059711398775	27.17243355541228	22.58620402018828
43	22.266448284130202	27.09772029698193	28.39771136671943	22.238120052168433
44	21.92085039651347	28.902810939146313	28.229541112082646	20.946797552257575
45	20.27051825944277	30.30116574272797	27.081070100597938	22.347245897231325
46	22.81564307212806	28.32734874899453	27.65725194560325	21.199756233274158
47	21.83023930813691	27.706482879901706	28.81723743474448	21.646040377216906
48	22.57455869619476	26.17751072040395	29.234669961377435	22.013260622023857
49	21.519773662006248	26.24846214664538	29.5296303165729	22.702133874775477
50	20.7096712780187	28.113251430068686	29.519195321196683	21.657881970715938
51	20.319160385224702	29.28278870181134	27.930753501529757	22.467297411434203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2173.0
1	2603.5
2	3034.0
3	23721.0
4	44408.0
5	30798.5
6	17189.0
7	17943.5
8	18698.0
9	19889.0
10	21080.0
11	21516.5
12	21953.0
13	21772.0
14	21591.0
15	20911.0
16	20231.0
17	19371.0
18	18511.0
19	18231.5
20	17952.0
21	17933.5
22	17915.0
23	18892.0
24	19869.0
25	20198.0
26	25399.0
27	30271.0
28	34245.5
29	38220.0
30	43469.0
31	48718.0
32	56983.0
33	65248.0
34	75845.5
35	86443.0
36	93292.0
37	100141.0
38	112506.5
39	124872.0
40	147218.0
41	169564.0
42	195211.5
43	220859.0
44	236614.5
45	252370.0
46	257727.5
47	263085.0
48	253218.0
49	243351.0
50	228394.5
51	213438.0
52	205453.0
53	197468.0
54	179105.0
55	160742.0
56	148414.5
57	136087.0
58	122590.0
59	109093.0
60	101218.0
61	93343.0
62	81935.5
63	70528.0
64	61871.0
65	53214.0
66	43843.5
67	34473.0
68	30221.0
69	25969.0
70	21902.0
71	17835.0
72	15628.5
73	13422.0
74	11588.5
75	8111.0
76	6467.0
77	4937.0
78	3407.0
79	2584.5
80	1762.0
81	1404.0
82	1046.0
83	721.0
84	396.0
85	262.0
86	128.0
87	125.0
88	122.0
89	75.5
90	29.0
91	20.0
92	11.0
93	10.5
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3057021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.87042672020838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57848201203508	17.971234146414606
2	6.64289887843089	3.0385186401781623
3	2.43239442582207	1.6688969541122096
4	1.3558329318486788	1.2403391085076194
5	0.9697234027149849	1.1088994010332092
6	0.7231165300077151	0.992279016582769
7	0.6338635571187288	1.014771102358615
8	0.5003591731177459	0.9154742242058771
9	0.4556268051127839	0.9378341512285129
>10	5.65538493873328	30.801017463391815
>50	1.643997026823573	26.580286763591204
>100	0.40542014596653836	12.140098320564311
>500	0.0015950947473589246	0.2517485970543697
>1k	0.001160068907170127	0.4196915420528321
>5k	0.0	0.0
>10k+	1.4500861339626587E-4	0.9189105687238144
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27708	0.9063725764396122	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3212	0.10506960861570791	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.542316850293144E-5	0.0	0.0	0.03601545426086376	0.0
2	6.542316850293144E-5	0.0	0.0	0.12747704382796193	0.0
3	6.542316850293144E-5	0.0	0.0	0.21589645605967378	0.0
4	6.542316850293144E-5	0.0	0.0	0.3424902871128461	0.0
5	6.542316850293144E-5	0.0	0.0	0.5401991023287049	0.0
6	6.542316850293144E-5	0.0	0.0	1.1622753000388286	0.0
7	6.542316850293144E-5	0.0	0.0	1.4697641920026066	0.0
8	6.542316850293144E-5	0.0	0.0	2.295568136430859	0.0
9	6.542316850293144E-5	0.0	0.0	2.8035136166876184	0.0
10	6.542316850293144E-5	0.0	0.0	3.4849613398141526	0.0
11	6.542316850293144E-5	0.0	0.0	3.8985338995054337	0.0
12	6.542316850293144E-5	0.0	0.0	4.253781704476351	0.0
13	6.542316850293144E-5	0.0	0.0	4.4746830329264995	0.0
14	6.542316850293144E-5	0.0	0.0	4.593556930096326	0.0
15	6.542316850293144E-5	0.0	0.0	4.693490819984554	0.0
16	6.542316850293144E-5	0.0	0.0	4.843408010609021	0.0
17	6.542316850293144E-5	0.0	0.0	4.9935541823232485	0.0
18	6.542316850293144E-5	0.0	0.0	5.210431985910467	0.0
19	6.542316850293144E-5	0.0	0.0	5.322403738803233	0.0
20	9.813475275439718E-5	0.0	0.0	5.445235737667487	0.0
21	9.813475275439718E-5	0.0	0.0	5.576834441111134	0.0
22	9.813475275439718E-5	0.0	0.0	5.732247177889848	0.0
23	9.813475275439718E-5	0.0	0.0	5.898585583808551	0.0
24	9.813475275439718E-5	0.0	0.0	6.039572511932368	0.0
25	9.813475275439718E-5	0.0	0.0	6.166885997839072	0.0
26	9.813475275439718E-5	0.0	0.0	6.287068358378958	0.0
27	9.813475275439718E-5	0.0	0.0	6.416671655183265	0.0
28	9.813475275439718E-5	0.0	0.0	6.544573949606496	0.0
29	9.813475275439718E-5	0.0	0.0	6.683500047922471	0.0
30	9.813475275439718E-5	0.0	0.0	6.883335116114675	0.0
31	1.308463370058629E-4	0.0	0.0	7.031616727526569	0.0
32	1.308463370058629E-4	0.0	0.0	7.206492856934905	0.0
33	1.308463370058629E-4	0.0	0.0	7.346694707036687	0.0
34	1.308463370058629E-4	0.0	0.0	7.490887370417148	0.0
35	1.308463370058629E-4	0.0	0.0	7.649669400373763	0.0
36	1.308463370058629E-4	0.0	0.0	7.797820165448651	0.0
37	1.308463370058629E-4	0.0	0.0	7.946788720129826	0.0
38	1.308463370058629E-4	0.0	0.0	8.112800010206014	0.0
39	1.308463370058629E-4	0.0	0.0	8.339949251248193	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	25	3.8923314E-5	45.0	29
CGTTTTT	18245	0.0	43.211838	1
AGGGCGA	4035	0.0	38.921936	6
GGGCGAT	8050	0.0	38.291924	7
CGTAAGG	465	0.0	38.225803	2
ACGGGTA	740	0.0	38.006756	5
CGGGTAT	500	0.0	37.800003	6
TACGGGT	640	0.0	37.617188	4
TAGGGTA	2420	0.0	37.561985	5
TAGGGTC	2220	0.0	37.094597	5
TGGGCGA	2190	0.0	37.08904	6
CGTTAGG	540	0.0	37.083336	2
GTTTTTT	21710	0.0	36.936897	2
TAAGGGT	2690	0.0	36.88662	4
TAGGGCG	1805	0.0	36.77285	5
ATAGGGT	2880	0.0	36.640625	4
CACGACC	1870	0.0	36.577538	27
AGGGTAC	2170	0.0	36.497696	6
TAGGGAC	2380	0.0	36.39706	5
TATACTA	1785	0.0	36.302525	44
>>END_MODULE