FastQCFastQC Report
Thu 26 May 2016
SRR1547649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547649_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3373367
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310940.9217496940000895No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38110.1129731808012588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT251650.043.36381
ACGGGTC5350.040.794395
CGAGGGA17700.038.389834
TACGGGA9050.038.038674
GTTTTTT294900.037.6068152
GGGCGAT83700.037.1505367
CGTTAGG5400.037.0833362
AGGGCGA44550.037.070716
CGAGGGT10500.036.8571434
CGTTCGG4300.036.62790745
CGTAAGG6400.036.2109382
ACGGGTA5350.036.1682245
TAGGGTC17250.036.1304325
ACGTTAG1750.036.01
GCGCGAC3250.036.09
TAGGGCG18800.035.9042555
AACGGGT5900.035.8474544
TATCGCG1450.035.6896551
GGCGATT21850.035.5263188
CGGGTCA4750.035.5263146
Produced by FastQC (version 0.11.5)