FastQCFastQC Report
Thu 26 May 2016
SRR1547648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547648_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1342354
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376002.801049499610386No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27320.2035230647057334No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25300.18847487324506054No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTT25220.18787890526642004No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCT19020.1416913869217807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17110.12746265143173857No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.11561778785625848No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTG15510.11554329185892842No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTC15480.11531980386693824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.1657743E-645.0000041
CGCATCG302.1657743E-645.00000421
GATCCGC302.1657743E-645.0000049
CGTTCGG302.1657743E-645.00000445
TACATCG302.1657743E-645.00000416
TACGCCT302.1657743E-645.00000434
TCCACGG351.2121382E-745.0000042
TCGAGCG302.1657743E-645.0000041
CGATAGA650.045.00000410
TCCGGAC207.0337113E-445.016
CGTGGTC207.0337113E-445.022
AGTCCGC253.891157E-545.040
GTCGACC207.0337113E-445.017
GGTCGAC207.0337113E-445.09
CGTGCCC207.0337113E-445.020
GAGTCGA207.0337113E-445.015
ACTTCGA207.0337113E-445.023
CATCTCG406.8157533E-945.022
TGCGGTC207.0337113E-445.029
CGATTAC253.891157E-545.010