##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547648_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1342354
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225935185502482	31.0	31.0	33.0	30.0	34.0
2	31.594778277563147	31.0	31.0	34.0	30.0	34.0
3	31.489510218615955	31.0	31.0	34.0	30.0	34.0
4	35.40479933013199	37.0	35.0	37.0	33.0	37.0
5	35.35642460930574	37.0	35.0	37.0	33.0	37.0
6	35.418238408050335	37.0	35.0	37.0	33.0	37.0
7	35.87918388145005	37.0	35.0	37.0	35.0	37.0
8	35.92393288208625	37.0	35.0	37.0	35.0	37.0
9	37.74453534611585	39.0	37.0	39.0	35.0	39.0
10	37.10839465595514	39.0	37.0	39.0	33.0	39.0
11	36.79280875238573	39.0	35.0	39.0	32.0	39.0
12	36.09841219231291	38.0	35.0	39.0	32.0	39.0
13	35.84938995227787	38.0	35.0	39.0	31.0	39.0
14	36.871362546690364	39.0	35.0	41.0	31.0	41.0
15	37.136572766945235	39.0	35.0	41.0	32.0	41.0
16	37.25024173951134	39.0	35.0	41.0	32.0	41.0
17	37.16804360101732	39.0	35.0	41.0	32.0	41.0
18	36.98055356485696	38.0	35.0	40.0	32.0	41.0
19	36.83598886731816	38.0	35.0	40.0	32.0	41.0
20	36.556090271269724	38.0	35.0	40.0	32.0	41.0
21	36.353013437588	37.0	35.0	40.0	31.0	41.0
22	36.279602846939035	37.0	35.0	40.0	31.0	41.0
23	36.220023928114344	37.0	35.0	40.0	31.0	41.0
24	36.07855230438469	37.0	35.0	40.0	31.0	41.0
25	35.943119326198605	36.0	35.0	40.0	31.0	41.0
26	35.80853932718195	36.0	35.0	40.0	31.0	41.0
27	35.722391410909495	36.0	35.0	40.0	30.0	41.0
28	35.655775600177	36.0	35.0	40.0	30.0	41.0
29	35.63370616096797	36.0	35.0	40.0	30.0	41.0
30	35.50643570920934	36.0	34.0	40.0	30.0	41.0
31	35.30529279161831	36.0	34.0	40.0	29.0	41.0
32	35.01276935890235	35.0	34.0	40.0	27.0	41.0
33	34.75590641514832	35.0	34.0	40.0	25.0	41.0
34	34.540464735829744	35.0	34.0	40.0	24.0	41.0
35	34.26524970313345	35.0	33.0	40.0	23.0	41.0
36	34.08501781199296	35.0	33.0	40.0	22.0	41.0
37	33.98753011500692	35.0	33.0	40.0	22.0	41.0
38	33.893589917413735	35.0	33.0	40.0	22.0	41.0
39	33.84694424868552	35.0	33.0	40.0	22.0	41.0
40	33.717724981636735	35.0	33.0	40.0	21.0	41.0
41	33.641855278115905	35.0	33.0	40.0	21.0	41.0
42	33.57587491824064	35.0	33.0	40.0	21.0	41.0
43	33.494403115720594	35.0	33.0	40.0	20.0	41.0
44	33.35965252087006	35.0	33.0	40.0	19.0	41.0
45	33.270482301985915	35.0	33.0	40.0	20.0	41.0
46	33.216394483124425	35.0	33.0	40.0	20.0	41.0
47	33.16645013163443	35.0	33.0	40.0	20.0	41.0
48	33.03231189388194	35.0	33.0	40.0	18.0	41.0
49	32.98741092141119	35.0	32.0	39.0	18.0	41.0
50	32.87303274695051	35.0	32.0	39.0	18.0	41.0
51	32.48329129275884	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	0.0
13	4.0
14	9.0
15	33.0
16	120.0
17	288.0
18	726.0
19	1447.0
20	2602.0
21	4314.0
22	6796.0
23	10443.0
24	16323.0
25	23115.0
26	29961.0
27	32081.0
28	32141.0
29	33339.0
30	38115.0
31	44967.0
32	55097.0
33	70571.0
34	116673.0
35	196063.0
36	118569.0
37	112101.0
38	145011.0
39	251325.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.770984405007916	23.75140983674947	27.431959080838585	19.045646677404022
2	34.264731955952	25.78336265992428	27.930486294971374	12.021419089152339
3	25.604423274337467	24.370918550546282	37.24658324108246	12.778074934033794
4	23.261449662309644	26.558865992130244	36.86710063068311	13.312583714876999
5	21.468479998569677	30.56436677657309	35.66629964971982	12.300853575137408
6	19.329252939239574	38.308821667011834	32.23583346866773	10.126091925080866
7	82.05048742731053	3.1694322064075497	12.830296628162168	1.9497837381197507
8	81.81366465179826	3.227017612343689	12.406563395348767	2.5527543405092845
9	77.60881257850015	5.5855608878134975	14.231938817927311	2.573687715759032
10	41.236439866085995	30.03462573955901	18.69231216206753	10.03662223228746
11	35.573105157059906	22.77476731175234	28.19092430163727	13.461203229550476
12	34.30712017843282	23.028724166650527	27.552195620529307	15.111960034387353
13	18.447890794827593	38.44023260630207	29.11564311649535	13.996233482374992
14	12.976532270921085	40.38852642447522	30.706505139478857	15.928436165124849
15	11.500319587828546	28.629854717906007	45.31531920789896	14.554506486366487
16	12.98390737465676	26.359738191266985	45.00824670690444	15.64810772717182
17	13.435129630484955	28.335595528452256	30.82465579124434	27.40461904981845
18	16.691051689792708	30.89967326055571	35.76202700628895	16.64724804336263
19	21.361801730393026	33.267901015678426	27.579386659554782	17.790910594373766
20	23.94576989378361	29.829389266914692	29.170099690543626	17.054741148758076
21	17.204776087380825	34.35695800064663	31.161973667154864	17.276292244817686
22	17.931335549340936	29.79832443602805	29.403197666189396	22.867142348441618
23	14.619541492035633	34.3293944816345	29.04137060715728	22.00969341917259
24	15.751880651452597	29.887943120816118	39.17431616399251	15.185860063738776
25	13.587101465038284	32.21929535726045	35.13134389289264	19.06225928480863
26	13.8988672138646	38.23842294953492	30.415747261899618	17.446962574700862
27	14.008376329939793	38.79982478541428	31.001211304916588	16.19058757972934
28	12.448355649850933	33.705937479979205	37.97254673506392	15.873160135105941
29	13.003350829959906	28.749867769604737	38.22344925407158	20.023332146363774
30	14.880500970682844	35.976277494610216	33.36668270813809	15.776538826568848
31	21.806840818442826	32.57709963243675	27.992466964749983	17.623592584370442
32	21.82248497788214	32.269654651455575	29.085174253587354	16.82268611707493
33	20.499585057294873	32.761402729831325	28.217593868681433	18.521418344192366
34	14.254213121129004	32.96947005037419	30.28895507444385	22.487361754052955
35	14.65202174687154	32.199106941984006	32.33260376919948	20.81626754194497
36	22.35684476673068	31.079581094107812	29.507045086467503	17.056529052694
37	15.443169238516816	36.0988979062155	30.980352425664172	17.477580429603517
38	15.222065118441186	37.48653484848259	28.018689555810166	19.272710477266056
39	16.196100283531766	36.07863499494172	29.53870588533278	18.18655883619373
40	19.871583799802437	33.15429461975008	27.761380381032126	19.212741199415355
41	14.738735087763736	30.854975662157674	30.064871114475018	24.341418135603572
42	18.74803516807042	31.218516129128382	26.27563221028134	23.757816492519858
43	18.683372642387923	31.907902088420787	27.461682983773283	21.947042285418007
44	16.26247621715285	35.50509031149756	28.54172595306454	19.69070751828504
45	14.36923494100662	40.638311503522914	25.021939071213705	19.97051448425676
46	18.823201629376456	37.24211348124265	26.294330705611184	17.640354183769706
47	17.33991182653756	33.24309384856752	27.913054231596135	21.503940093298787
48	18.478061673746268	30.111356616808976	31.677485968678905	19.733095740765847
49	17.26802318911405	29.275735014757657	31.095523237536447	22.360718558591845
50	15.664869326571083	35.00805301731138	30.521457082110974	18.80562057400656
51	14.639506419320089	36.07595313903784	27.521950245613304	21.762590196028768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2031.0
1	2078.5
2	2126.0
3	22691.5
4	43257.0
5	27523.5
6	11790.0
7	12440.5
8	13091.0
9	16606.5
10	20122.0
11	23572.5
12	27023.0
13	27910.5
14	28798.0
15	26322.5
16	23847.0
17	21386.0
18	18925.0
19	17072.5
20	15220.0
21	14180.5
22	13141.0
23	12118.5
24	11096.0
25	12143.0
26	13730.0
27	14270.0
28	16686.0
29	19102.0
30	22358.5
31	25615.0
32	29813.0
33	34011.0
34	39240.5
35	44470.0
36	46085.0
37	47700.0
38	53050.0
39	58400.0
40	72398.5
41	86397.0
42	98934.5
43	111472.0
44	118575.0
45	125678.0
46	127780.0
47	129882.0
48	127665.0
49	125448.0
50	109162.5
51	92877.0
52	78060.0
53	63243.0
54	51444.0
55	39645.0
56	33141.5
57	26638.0
58	23264.5
59	19891.0
60	16217.0
61	12543.0
62	9868.5
63	7194.0
64	6374.5
65	5555.0
66	4307.5
67	3060.0
68	2608.0
69	2156.0
70	1664.0
71	1172.0
72	962.5
73	753.0
74	617.0
75	451.5
76	422.0
77	362.5
78	303.0
79	235.5
80	168.0
81	144.0
82	120.0
83	66.5
84	13.0
85	10.0
86	7.0
87	6.5
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1342354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.58029973862968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08018842458074	20.222342124967806
2	9.033142469680655	4.802072688517148
3	3.8583223847416424	3.07666096424092
4	2.243599478444963	2.3854218652200134
5	1.4784983991821534	1.9649465306672897
6	0.9782802772739373	1.5601789798998607
7	0.7161217717922186	1.3324311940517015
8	0.5023145612702814	1.0681337281313876
9	0.3912016211074298	0.9358430712545992
>10	3.183489680056166	18.325952897174144
>50	0.9503835776973892	18.357466897673895
>100	0.5782216307044273	21.231337719110588
>500	0.003401303709838546	0.613717414170815
>1k	0.0025509777823789093	1.2907201988183654
>5k	0.0	0.0
>10k+	2.8344197581987884E-4	2.8327737261014794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37600	2.801049499610386	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2732	0.2035230647057334	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2530	0.18847487324506054	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTT	2522	0.18787890526642004	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCT	1902	0.1416913869217807	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1711	0.12746265143173857	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1552	0.11561778785625848	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTCTG	1551	0.11554329185892842	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTC	1548	0.11531980386693824	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0896931807853964	0.0
2	0.0	0.0	0.0	0.28800152567802534	0.0
3	0.0	0.0	0.0	0.5272081731048591	0.0
4	0.0	0.0	0.0	0.8706347207964517	0.0
5	0.0	0.0	0.0	1.54974023245731	0.0
6	0.0	0.0	0.0	2.722381726429839	0.0
7	0.0	0.0	0.0	3.507644034286038	0.0
8	0.0	0.0	0.0	4.87144225740751	0.0
9	0.0	0.0	0.0	5.4948247630654805	0.0
10	0.0	0.0	0.0	6.23427203256369	0.0
11	0.0	0.0	0.0	6.998377477178151	0.0
12	0.0	0.0	0.0	7.578999280368666	0.0
13	0.0	0.0	0.0	7.856943846407132	0.0
14	0.0	0.0	0.0	7.987162849740083	0.0
15	0.0	0.0	0.0	8.121479132926188	0.0
16	0.0	0.0	0.0	8.402478034855188	0.0
17	0.0	0.0	0.0	8.728919495155525	0.0
18	0.0	0.0	0.0	9.137679032505584	0.0
19	0.0	0.0	0.0	9.356101296677329	0.0
20	0.0	0.0	0.0	9.587113384397856	0.0
21	0.0	0.0	0.0	9.906701212943828	0.0
22	0.0	0.0	0.0	10.22539508952184	0.0
23	0.0	0.0	0.0	10.581113476772893	0.0
24	0.0	0.0	0.0	10.854066810990245	0.0
25	0.0	0.0	0.0	11.078523250945727	0.0
26	0.0	0.0	0.0	11.29195428329636	0.0
27	0.0	0.0	0.0	11.49473238802879	0.0
28	0.0	0.0	0.0	11.707194972414133	0.0
29	0.0	0.0	0.0	11.952435795624702	0.0
30	0.0	0.0	0.0	12.258092872669952	0.0
31	0.0	0.0	0.0	12.519126847314494	0.0
32	0.0	0.0	0.0	12.750585911019002	0.0
33	0.0	0.0	0.0	12.993368366317677	0.0
34	0.0	0.0	0.0	13.232202533757862	0.0
35	0.0	0.0	0.0	13.523258395326419	0.0
36	0.0	0.0	0.0	13.77118107444087	0.0
37	0.0	0.0	0.0	14.01776282560338	0.0
38	0.0	0.0	0.0	14.274997504384089	0.0
39	0.0	0.0	0.0	14.542363638801687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.1657743E-6	45.000004	1
CGCATCG	30	2.1657743E-6	45.000004	21
GATCCGC	30	2.1657743E-6	45.000004	9
CGTTCGG	30	2.1657743E-6	45.000004	45
TACATCG	30	2.1657743E-6	45.000004	16
TACGCCT	30	2.1657743E-6	45.000004	34
TCCACGG	35	1.2121382E-7	45.000004	2
TCGAGCG	30	2.1657743E-6	45.000004	1
CGATAGA	65	0.0	45.000004	10
TCCGGAC	20	7.0337113E-4	45.0	16
CGTGGTC	20	7.0337113E-4	45.0	22
AGTCCGC	25	3.891157E-5	45.0	40
GTCGACC	20	7.0337113E-4	45.0	17
GGTCGAC	20	7.0337113E-4	45.0	9
CGTGCCC	20	7.0337113E-4	45.0	20
GAGTCGA	20	7.0337113E-4	45.0	15
ACTTCGA	20	7.0337113E-4	45.0	23
CATCTCG	40	6.8157533E-9	45.0	22
TGCGGTC	20	7.0337113E-4	45.0	29
CGATTAC	25	3.891157E-5	45.0	10
>>END_MODULE