FastQCFastQC Report
Thu 26 May 2016
SRR1547647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547647_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1368636
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105190.768575428382711No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51170.3738758881104983No Hit
GAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT29180.21320497195748178Illumina PCR Primer Index 10 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT26660.19479247951975542No Hit
AAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT25670.18755900034779152Illumina PCR Primer Index 10 (95% over 21bp)
GAGAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT25100.18339426991544866No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT19870.1451810415625484No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC14580.10652942053255943Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT13700.10009966126859152Illumina PCR Primer Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG302.165807E-645.0000041
CGTATCG302.165807E-645.00000424
CGACGGT1450.045.00000428
TAGCACG551.8189894E-1245.0000041
TACGATA302.165807E-645.00000432
TATTACG302.165807E-645.0000041
TCCGTGA600.045.00000435
GTAAGCG302.165807E-645.0000041
CCCGGAG302.165807E-645.00000418
GTACGAT302.165807E-645.00000431
ACGCGAG453.8562575E-1045.01
TCACGAT207.033759E-445.018
TCTGCGC207.033759E-445.016
GCCCCTA207.033759E-445.045
AACGTTG351.2121563E-745.01
CTAGAAC351.2121563E-745.024
CGTGGAC207.033759E-445.041
GTCGCGA207.033759E-445.037
GCCCACG253.8911967E-545.040
CATATCC453.8562575E-1045.033