FastQCFastQC Report
Thu 26 May 2016
SRR1547646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547646_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1404574
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97780.696154136414315No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34650.24669401540965444No Hit
GAGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT15820.11263201511632707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.165847E-645.0000041
ATGTACG302.165847E-645.0000041
TCGTAAT302.165847E-645.00000430
CAATACG302.165847E-645.0000041
CTTAACG207.033823E-445.01
CTCGTTG351.2121927E-745.01
CGTATCG207.033823E-445.033
CACGGTA207.033823E-445.037
CTCCGAC253.891249E-545.013
CGACGGT1000.045.028
CGGTTTA207.033823E-445.037
TCTTACG253.891249E-545.017
TTCACCG207.033823E-445.029
CGTCTAC207.033823E-445.017
CGACATC207.033823E-445.021
ATCGTTA207.033823E-445.019
ATACTCG800.045.044
CCCGTTC253.891249E-545.035
ACTACGC253.891249E-545.021
GAACGCG253.891249E-545.01