##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547646_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1404574
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167188770402984	31.0	31.0	33.0	30.0	34.0
2	31.544416314127986	31.0	31.0	34.0	30.0	34.0
3	31.58807296731963	31.0	31.0	34.0	30.0	34.0
4	35.42222410495994	37.0	35.0	37.0	33.0	37.0
5	35.33321277483422	37.0	35.0	37.0	33.0	37.0
6	35.37779782339699	37.0	35.0	37.0	33.0	37.0
7	35.82095994942239	37.0	35.0	37.0	35.0	37.0
8	35.872965753317374	37.0	35.0	37.0	35.0	37.0
9	37.715043137634616	39.0	37.0	39.0	35.0	39.0
10	36.950707474294695	39.0	37.0	39.0	32.0	39.0
11	36.63499324350301	39.0	35.0	39.0	32.0	39.0
12	35.73460921247296	37.0	35.0	39.0	31.0	39.0
13	35.40411256366699	37.0	35.0	39.0	30.0	39.0
14	36.29898673903974	38.0	35.0	40.0	31.0	41.0
15	36.63005295555806	38.0	35.0	40.0	31.0	41.0
16	36.79474488350204	38.0	35.0	41.0	32.0	41.0
17	36.723569566288425	38.0	35.0	40.0	32.0	41.0
18	36.63424782175948	38.0	35.0	40.0	32.0	41.0
19	36.538841670143405	37.0	35.0	40.0	31.0	41.0
20	36.30885805945432	37.0	35.0	40.0	31.0	41.0
21	36.096994533573884	37.0	35.0	40.0	31.0	41.0
22	36.02323693874442	37.0	35.0	40.0	31.0	41.0
23	36.02035706199887	36.0	35.0	40.0	31.0	41.0
24	35.92756878598066	36.0	35.0	40.0	31.0	41.0
25	35.8185777324655	36.0	34.0	40.0	30.0	41.0
26	35.67788881183903	36.0	34.0	40.0	30.0	41.0
27	35.615660691426726	36.0	34.0	40.0	30.0	41.0
28	35.63520113571802	36.0	34.0	40.0	30.0	41.0
29	35.67329097648113	36.0	34.0	40.0	30.0	41.0
30	35.65964484605297	36.0	35.0	40.0	30.0	41.0
31	35.47387891275219	36.0	34.0	40.0	30.0	41.0
32	35.213027579892554	36.0	34.0	40.0	29.0	41.0
33	35.02658030121589	36.0	34.0	40.0	27.0	41.0
34	34.86381991977639	36.0	34.0	40.0	27.0	41.0
35	34.65892505485649	36.0	34.0	40.0	25.0	41.0
36	34.50779311022417	36.0	34.0	40.0	24.0	41.0
37	34.43070710407569	36.0	34.0	40.0	24.0	41.0
38	34.3784777448536	35.0	33.0	40.0	24.0	41.0
39	34.35889102318568	36.0	34.0	40.0	24.0	41.0
40	34.26232152951714	36.0	33.0	40.0	23.0	41.0
41	34.22178041171202	36.0	34.0	40.0	23.0	41.0
42	34.15013804897428	36.0	33.0	40.0	23.0	41.0
43	34.06764898111456	35.0	33.0	40.0	23.0	41.0
44	33.93955889828517	35.0	33.0	40.0	23.0	41.0
45	33.84087986820203	35.0	33.0	40.0	23.0	41.0
46	33.787706450496735	35.0	33.0	40.0	23.0	41.0
47	33.73168804206827	35.0	33.0	40.0	23.0	41.0
48	33.62299458768281	35.0	33.0	40.0	23.0	41.0
49	33.613518404868664	35.0	33.0	40.0	23.0	41.0
50	33.49061921977767	35.0	33.0	39.0	22.0	41.0
51	33.04967840783042	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	0.0
12	1.0
13	1.0
14	5.0
15	24.0
16	86.0
17	236.0
18	582.0
19	1242.0
20	2246.0
21	3642.0
22	5871.0
23	9022.0
24	14141.0
25	20669.0
26	27005.0
27	30511.0
28	31403.0
29	34457.0
30	40534.0
31	49422.0
32	60817.0
33	79224.0
34	137101.0
35	227906.0
36	97518.0
37	111068.0
38	157318.0
39	262431.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.042009890543326	24.8272429932492	29.948795862660138	14.18195125354734
2	30.937138235507707	26.075308242926326	29.61438841954927	13.373165102016696
3	27.079527315755524	25.18970164619308	34.28270778186126	13.448063256190132
4	23.700638058229757	27.931102241676125	33.562062233816086	14.806197466278032
5	22.0916804668177	31.411659335855568	32.751852163004585	13.744808034322151
6	19.314397105456884	39.86639365387655	30.19791054084726	10.621298699819306
7	85.81028838637195	3.2216173729543622	9.191327762011827	1.7767664786618576
8	86.37672347629957	2.306678039035323	8.263573154565014	3.0530253301000876
9	82.67083115592342	3.773813270073346	10.868562282941305	2.6867932910619166
10	48.624493974685564	20.561180827781232	17.77862896508123	13.035696232451974
11	41.52134383806051	21.76061923401686	23.245055084317382	13.47298184360525
12	38.86865341377528	19.9132975549882	25.62955031205191	15.588498719184607
13	19.552903585001573	39.98158872369843	26.83197894877735	13.633528742522644
14	14.216267708216158	41.43590868120868	29.212060026741206	15.135763583833958
15	12.773908672665163	26.324351725149402	47.646190232768085	13.255549369417347
16	13.515699422031163	20.9933403295234	47.64497990137935	17.845980347066085
17	14.119227609225288	23.524072067402642	29.432696319311052	32.92400400406102
18	19.75275065607081	25.03015149077229	35.79441168639032	19.422686166766578
19	24.872381234452583	30.443394224868182	26.311038079873327	18.37318646080591
20	28.287366845748245	24.658579754430882	28.037540207920692	19.016513191900177
21	18.74091361508899	31.06201595643946	30.26376680758721	19.93330362088434
22	19.514600156346336	24.985582817281255	28.925069095683103	26.57474793068931
23	16.122753233364705	31.823314399953297	26.325206076717922	25.728726289964076
24	17.99741416258595	24.558051053201897	40.08510765541723	17.359427128794923
25	14.709157367287162	27.52201023228395	35.819757449589694	21.949074950839186
26	14.183659956684375	37.55615581663906	29.18108978238242	19.079094444294142
27	15.828286726082071	36.255975121282326	29.96153994022387	17.954198212411736
28	13.690912689541454	30.840169332480883	39.06266241579298	16.40625556218469
29	13.618933569893791	25.418596670591935	39.04493462074622	21.917535138768056
30	16.212887323843386	33.55081327149726	32.71710853255151	17.519190872107842
31	25.820640279543834	28.911114686730638	26.728460017058552	18.539785016666976
32	25.141288390643712	28.857717713698243	28.590448064680107	17.410545830977934
33	24.67374449477208	29.75927220637716	25.46793547367387	20.099047825176886
34	16.052696404746207	29.80590556282545	29.114521556002032	25.026876476426303
35	16.120759746371498	28.27796897849455	31.099393837562133	24.50187743757182
36	26.006604137624645	25.589395788331554	29.75542762431883	18.64857244972497
37	16.57563076064344	31.926334959923793	32.621705940733634	18.876328338699135
38	16.231327078530573	32.67659802901093	27.264708018231865	23.827366874226634
39	16.76152342276021	31.301234395624583	31.30415343015035	20.633088751464857
40	21.56753577953173	27.705126251803037	27.83569964985825	22.891638318806983
41	14.994581987136312	24.98280617468357	31.192233374674455	28.830378463505664
42	20.530993739026922	25.734493163051575	27.487266601830875	26.247246496090632
43	20.63828605684001	26.20901426339944	28.574072992950178	24.578626686810377
44	17.42179479329676	31.69551764449577	28.738037298141645	22.14465026406583
45	15.762786439162339	38.033453559584615	24.408539528711195	21.79522047254185
46	21.270007845795238	34.14181096901979	25.26089761023627	19.3272835749487
47	18.538574685278242	29.34939704138052	27.792483699684034	24.319544573657208
48	20.733403864801712	24.739956741332247	32.81607092257154	21.7105684712945
49	19.337179813950705	23.75253991601724	33.280624587953355	23.629655682078692
50	16.728346103516085	31.617985239652736	30.35924059536913	21.29442806146205
51	15.829924233255067	33.69206606415895	25.39659711770259	25.081412584883388
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1064.0
1	1272.0
2	1480.0
3	8116.5
4	14753.0
5	10994.0
6	7235.0
7	7657.0
8	8079.0
9	9248.5
10	10418.0
11	11059.0
12	11700.0
13	11684.5
14	11669.0
15	11095.0
16	10521.0
17	9679.0
18	8837.0
19	8559.5
20	8282.0
21	8466.0
22	8650.0
23	8670.5
24	8691.0
25	8655.5
26	10663.0
27	12706.0
28	14222.0
29	15738.0
30	17738.5
31	19739.0
32	24619.5
33	29500.0
34	35094.5
35	40689.0
36	41637.0
37	42585.0
38	53284.5
39	63984.0
40	82457.5
41	100931.0
42	124692.0
43	148453.0
44	152590.0
45	156727.0
46	161710.5
47	166694.0
48	158520.0
49	150346.0
50	133774.0
51	117202.0
52	99233.5
53	81265.0
54	65394.5
55	49524.0
56	40729.0
57	31934.0
58	26518.0
59	21102.0
60	18082.5
61	15063.0
62	12283.0
63	9503.0
64	7268.0
65	5033.0
66	3644.5
67	2256.0
68	1857.0
69	1458.0
70	1185.0
71	912.0
72	710.5
73	509.0
74	372.5
75	303.5
76	371.0
77	214.5
78	58.0
79	43.5
80	29.0
81	23.0
82	17.0
83	10.0
84	3.0
85	2.5
86	2.0
87	3.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1404574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.322384598618083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1626493034897	21.854302302217253
2	10.11942737346642	5.732126279781992
3	3.5497222890195497	3.016097996636958
4	1.823501777620837	2.0658367464816436
5	1.056606179402119	1.4962803291151638
6	0.7252572499889193	1.2324608860273363
7	0.5481615845578436	1.0867670254024608
8	0.3931177579231816	0.8907225865957432
9	0.34315746752690585	0.874713399586634
>10	2.844868983335339	17.578655698672605
>50	0.8056317229997831	16.65386170849875
>100	0.6230604891373976	25.33929017210258
>500	0.002036977487163644	0.39309927253158244
>1k	0.0025462218589545555	1.0806443953193472
>5k	2.546221858954555E-4	0.7051412010298684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9778	0.696154136414315	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3465	0.24669401540965444	No Hit
GAGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	1582	0.11263201511632707	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1358789212957096E-4	0.0	0.0	0.1117776635478088	0.0
2	2.1358789212957096E-4	0.0	0.0	0.3717141282694967	0.0
3	2.1358789212957096E-4	0.0	0.0	0.6193336912117126	0.0
4	2.1358789212957096E-4	0.0	0.0	1.1753029744249859	0.0
5	2.1358789212957096E-4	0.0	0.0	2.007726186017967	0.0
6	2.1358789212957096E-4	0.0	0.0	3.2305168684597607	0.0
7	2.1358789212957096E-4	0.0	0.0	3.7667648696330702	0.0
8	2.1358789212957096E-4	0.0	0.0	5.214463602487302	0.0
9	2.1358789212957096E-4	0.0	0.0	5.704007051248279	0.0
10	2.1358789212957096E-4	0.0	0.0	6.596804440349885	0.0
11	2.1358789212957096E-4	0.0	0.0	7.6726466530065345	0.0
12	2.1358789212957096E-4	0.0	0.0	8.454093554344592	0.0
13	2.1358789212957096E-4	0.0	0.0	8.840901226991244	0.0
14	2.1358789212957096E-4	0.0	0.0	8.982438803509107	0.0
15	2.1358789212957096E-4	0.0	0.0	9.184848929283897	0.0
16	2.1358789212957096E-4	0.0	0.0	9.664780922899043	0.0
17	2.1358789212957096E-4	0.0	0.0	10.24246497514549	0.0
18	2.1358789212957096E-4	0.0	0.0	10.91975218108836	0.0
19	2.1358789212957096E-4	0.0	0.0	11.297446770337483	0.0
20	2.1358789212957096E-4	0.0	0.0	11.650080380243406	0.0
21	2.1358789212957096E-4	0.0	0.0	12.207687170629672	0.0
22	2.1358789212957096E-4	0.0	0.0	12.758672736359921	0.0
23	2.1358789212957096E-4	0.0	0.0	13.490994422508177	0.0
24	2.1358789212957096E-4	0.0	0.0	13.945367065031817	0.0
25	2.8478385617276126E-4	0.0	0.0	14.348336221516275	0.0
26	2.8478385617276126E-4	0.0	0.0	14.710937266388243	0.0
27	2.8478385617276126E-4	0.0	0.0	15.07909159645558	0.0
28	2.8478385617276126E-4	0.0	0.0	15.48725805831519	0.0
29	2.8478385617276126E-4	0.0	0.0	15.905320759176805	0.0
30	2.8478385617276126E-4	0.0	0.0	16.401770216449968	0.0
31	2.8478385617276126E-4	0.0	0.0	16.861126576456634	0.0
32	2.8478385617276126E-4	0.0	0.0	17.279046885390162	0.0
33	2.8478385617276126E-4	0.0	0.0	17.715620536903003	0.0
34	2.8478385617276126E-4	0.0	0.0	18.103496149010304	0.0
35	2.8478385617276126E-4	0.0	0.0	18.568121010356165	0.0
36	2.8478385617276126E-4	0.0	0.0	18.99444244304679	0.0
37	2.8478385617276126E-4	0.0	0.0	19.427669884249603	0.0
38	2.8478385617276126E-4	0.0	0.0	19.85833427074686	0.0
39	2.8478385617276126E-4	0.0	0.0	20.28750354199921	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.165847E-6	45.000004	1
ATGTACG	30	2.165847E-6	45.000004	1
TCGTAAT	30	2.165847E-6	45.000004	30
CAATACG	30	2.165847E-6	45.000004	1
CTTAACG	20	7.033823E-4	45.0	1
CTCGTTG	35	1.2121927E-7	45.0	1
CGTATCG	20	7.033823E-4	45.0	33
CACGGTA	20	7.033823E-4	45.0	37
CTCCGAC	25	3.891249E-5	45.0	13
CGACGGT	100	0.0	45.0	28
CGGTTTA	20	7.033823E-4	45.0	37
TCTTACG	25	3.891249E-5	45.0	17
TTCACCG	20	7.033823E-4	45.0	29
CGTCTAC	20	7.033823E-4	45.0	17
CGACATC	20	7.033823E-4	45.0	21
ATCGTTA	20	7.033823E-4	45.0	19
ATACTCG	80	0.0	45.0	44
CCCGTTC	25	3.891249E-5	45.0	35
ACTACGC	25	3.891249E-5	45.0	21
GAACGCG	25	3.891249E-5	45.0	1
>>END_MODULE