FastQCFastQC Report
Thu 26 May 2016
SRR1547645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547645_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2591832
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316881.2226101074452358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACG351.2127202E-745.00000414
CGTTAAC207.034928E-445.013
CGTTTTT187450.043.6676451
CGACGGT2200.041.9318228
CGCATCG700.041.78571721
ACGGGTA1850.041.3513535
TACGGGA10250.039.951224
ATAGGGA45400.038.8546264
TAGGGAT53400.038.6797755
TAGGGAC33300.038.581085
TCGCGCG356.2513245E-638.571431
GGGCGAT37050.038.4412967
CGCATTA659.094947E-1238.0769227
GTTAGCG1300.038.076921
CGGTCTA2450.037.6530631
TTAGGGA41900.037.58954
CTACGGG4500.037.53
TCGACGG1500.037.52
AGTCCGC301.1403173E-437.49999611
TCGCGCA2950.037.37288334
Produced by FastQC (version 0.11.5)