##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547645_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2591832
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293588087499497	31.0	31.0	33.0	30.0	34.0
2	31.701336737874986	31.0	31.0	34.0	30.0	34.0
3	31.694852521305393	31.0	31.0	34.0	30.0	34.0
4	35.55454172955655	37.0	35.0	37.0	33.0	37.0
5	35.487430126644014	37.0	35.0	37.0	33.0	37.0
6	35.538857070983	37.0	35.0	37.0	33.0	37.0
7	35.92122367499128	37.0	35.0	37.0	35.0	37.0
8	35.965753953188326	37.0	35.0	37.0	35.0	37.0
9	37.792083746168736	39.0	38.0	39.0	35.0	39.0
10	37.16100271931205	39.0	37.0	39.0	33.0	39.0
11	36.84928035459088	39.0	37.0	39.0	32.0	39.0
12	36.471915617987584	39.0	35.0	39.0	32.0	39.0
13	36.353387873905405	39.0	35.0	39.0	32.0	39.0
14	37.420332027693156	40.0	36.0	41.0	32.0	41.0
15	37.60711689646551	40.0	36.0	41.0	32.0	41.0
16	37.64728076511132	40.0	36.0	41.0	33.0	41.0
17	37.54213621870554	39.0	35.0	41.0	32.0	41.0
18	37.380190151213505	39.0	36.0	41.0	32.0	41.0
19	37.274494643171316	39.0	36.0	41.0	32.0	41.0
20	37.06376532120909	39.0	35.0	41.0	32.0	41.0
21	36.90572537108887	39.0	35.0	41.0	31.0	41.0
22	36.86280013519395	38.0	35.0	41.0	31.0	41.0
23	36.78715364267437	38.0	35.0	41.0	31.0	41.0
24	36.68014400624732	38.0	35.0	40.0	31.0	41.0
25	36.5752606650431	38.0	35.0	40.0	31.0	41.0
26	36.47821039326623	38.0	35.0	40.0	31.0	41.0
27	36.40414579340019	38.0	35.0	40.0	31.0	41.0
28	36.388125079094635	38.0	35.0	40.0	30.0	41.0
29	36.388112346787906	38.0	35.0	40.0	30.0	41.0
30	36.345412819966725	38.0	35.0	40.0	30.0	41.0
31	36.21985182681593	38.0	35.0	40.0	30.0	41.0
32	36.013950749894285	38.0	35.0	41.0	30.0	41.0
33	35.77732738850358	38.0	35.0	41.0	29.0	41.0
34	35.54604735183453	38.0	35.0	41.0	27.0	41.0
35	35.27469064352936	38.0	34.0	41.0	25.0	41.0
36	35.166297043944205	38.0	34.0	40.0	24.0	41.0
37	35.117996459647074	38.0	34.0	40.0	24.0	41.0
38	35.00985519123153	38.0	34.0	40.0	24.0	41.0
39	34.938695872263324	38.0	34.0	40.0	23.0	41.0
40	34.8734879421197	38.0	34.0	40.0	23.0	41.0
41	34.7731338296618	38.0	34.0	40.0	23.0	41.0
42	34.7298343411147	38.0	34.0	40.0	23.0	41.0
43	34.683089799030185	38.0	34.0	40.0	23.0	41.0
44	34.5557871806506	38.0	34.0	40.0	23.0	41.0
45	34.48072676006778	38.0	34.0	40.0	23.0	41.0
46	34.456336290315114	38.0	34.0	40.0	23.0	41.0
47	34.42037871281781	37.0	33.0	40.0	23.0	41.0
48	34.28008798409773	37.0	33.0	40.0	23.0	41.0
49	34.21541403918155	37.0	33.0	40.0	22.0	41.0
50	34.144862012661314	37.0	33.0	40.0	22.0	41.0
51	33.75453887443322	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	0.0
11	2.0
12	7.0
13	7.0
14	23.0
15	71.0
16	176.0
17	546.0
18	1169.0
19	2297.0
20	4233.0
21	6496.0
22	10386.0
23	15581.0
24	23777.0
25	36529.0
26	49604.0
27	55888.0
28	56391.0
29	58573.0
30	64705.0
31	75455.0
32	92060.0
33	117173.0
34	179190.0
35	258852.0
36	188740.0
37	239109.0
38	371207.0
39	683327.0
40	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.80000246929585	23.649989659823632	28.70494692557234	17.845060945308184
2	33.86488784766914	24.835328833041647	28.582369536297104	12.717413782992107
3	26.833375002700794	24.70727269360051	35.36679846533263	13.092553838366067
4	23.923502757894802	27.21908673093009	34.75510758413354	14.102302927041569
5	22.067248185839205	31.04036064065881	33.81006176326243	13.082329410239552
6	20.667041690973797	39.08447769762855	30.224104031434134	10.024376579963516
7	86.0122106679754	2.6390985218177723	10.018782081554669	1.3299087286521656
8	86.839849187756	2.0459659422369967	9.614280555221171	1.4999043147858349
9	82.19815173205671	4.76400476574099	10.918608922183228	2.119234580019075
10	38.19456662314533	37.01181249401967	15.9740291809037	8.819591701931298
11	27.30377586201575	23.53188786927548	32.59833199065372	16.56600427805506
12	28.71115874794354	22.21351538216983	31.78809429006201	17.28723157982462
13	22.26733831513771	28.066325286515486	31.975645026375165	17.69069137197164
14	16.429961509850948	31.3705132122761	33.03157766398439	19.167947613888554
15	16.12052787372021	27.069192756320625	40.2624861487936	16.54779322116557
16	19.699039135252594	25.090399377737445	38.65119344155023	16.559368045459735
17	19.765478626701114	25.21305393250797	32.00898052034237	23.012486920448545
18	20.827623086681545	25.73469268069844	35.065698702693695	18.37198552992632
19	23.136067461162607	27.653219807456658	30.90968087437766	18.30103185700308
20	24.516095179008516	26.53624926306952	31.619834927572466	17.327820630349496
21	21.964540911602295	26.660447127745933	33.55892665882666	17.81608530182512
22	22.04274814108322	23.71959293657922	33.13424635547366	21.103412566863902
23	18.92368023853398	27.587899215689905	32.74579525216141	20.74262529361471
24	18.868699823136684	26.324352812990963	37.09082996120119	17.71611740267116
25	18.74048935270496	27.911106892730704	33.93503128289179	19.413372471672545
26	18.0303738822578	31.315880041607635	31.2955083508499	19.358237725284663
27	17.655195244136195	30.301616771457407	33.45926742165387	18.583920562752525
28	16.70127539130623	28.29890208933295	36.85296732195605	18.146855197404772
29	17.543575355192775	25.719182416144253	36.535817136295876	20.2014250923671
30	18.8177320134947	28.092908799644423	34.237134196969556	18.85222498989132
31	22.427148055892513	27.229041079823073	31.902106309359556	18.441704554924858
32	22.54254905410536	27.650017439401935	31.176210495124685	18.63122301136802
33	21.973839353785277	28.242262615786828	31.024541714123444	18.75935631630445
34	19.056173393954545	28.41164087795814	31.820658129076268	20.71152759901105
35	19.282422626157867	28.40828417891283	32.045402634121345	20.263890560807955
36	22.38814089802117	28.21440587198553	30.312342775303335	19.085110454689964
37	19.859581948212693	29.93785862663938	31.676242904632705	18.526316520515216
38	19.00802212489081	30.243009577781276	30.420914627182626	20.328053670145287
39	19.494087579750538	30.24169776436127	30.795591689584818	19.46862296630337
40	22.314949425734383	27.540133774102642	30.239459964997735	19.905456835165243
41	18.511655076409273	27.113408585124343	31.5102213415067	22.864714996959677
42	20.190814836764112	27.405287071075595	29.864281326876124	22.53961676528417
43	20.603148660869994	28.13643013899049	29.905449118615714	21.3549720815238
44	19.21405399732699	30.278042712644954	29.866249047006132	20.64165424302192
45	18.20160411631618	31.913140975186664	28.62785859577318	21.257396312723973
46	20.309572534022266	30.788453881270083	29.21366045330099	19.688313131406666
47	19.635609098120558	28.666672839906287	30.898954870531732	20.79876319144142
48	20.229860577383104	26.901319221307553	32.25818648739579	20.610633713913558
49	19.855067766737967	26.938088579815357	31.7062216995546	21.500621953892075
50	18.45250772426608	29.535170489445306	31.977921408486353	20.034400377802264
51	18.068377888690314	29.812310365795312	30.593765336642193	21.525546408872177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1491.0
1	2201.5
2	2912.0
3	26918.5
4	50925.0
5	36030.0
6	21135.0
7	20651.5
8	20168.0
9	20557.5
10	20947.0
11	21027.0
12	21107.0
13	20769.0
14	20431.0
15	19503.0
16	18575.0
17	17748.5
18	16922.0
19	16456.5
20	15991.0
21	15965.5
22	15940.0
23	18000.0
24	20060.0
25	22587.5
26	30296.0
27	35477.0
28	41206.0
29	46935.0
30	54714.0
31	62493.0
32	70407.5
33	78322.0
34	89292.0
35	100262.0
36	106971.0
37	113680.0
38	124593.5
39	135507.0
40	152021.0
41	168535.0
42	187837.0
43	207139.0
44	211350.5
45	215562.0
46	218219.5
47	220877.0
48	212500.5
49	204124.0
50	189368.0
51	174612.0
52	160160.5
53	145709.0
54	127983.5
55	110258.0
56	99274.5
57	88291.0
58	78347.5
59	68404.0
60	59913.5
61	51423.0
62	43127.0
63	34831.0
64	29136.5
65	23442.0
66	18739.5
67	14037.0
68	11286.0
69	8535.0
70	6902.0
71	5269.0
72	3973.0
73	2677.0
74	2228.0
75	1380.5
76	982.0
77	757.5
78	533.0
79	364.0
80	195.0
81	171.0
82	147.0
83	88.0
84	29.0
85	30.5
86	32.0
87	20.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2591832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.721593302386406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44561226920665	16.822420927660136
2	7.2072327172143815	3.131051558379682
3	2.7326509453440444	1.7807259741643524
4	1.5376740364858306	1.3360292020873636
5	1.0224310882851524	1.1104416139723206
6	0.7324023494232981	0.9545367580731108
7	0.5463646242113958	0.8307537113351783
8	0.44474108333054363	0.772838794957505
9	0.3852034225691308	0.7530508875360556
>10	5.5856287390837425	30.283324046471687
>50	1.9808891626059082	30.189424804196673
>100	0.37664182064208795	10.244035493983242
>500	0.001624976741533665	0.26512031911190653
>1k	7.222118851260733E-4	0.28347480274273085
>5k	0.0	0.0
>10k+	1.8055297128151832E-4	1.2427711053281205
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31688	1.2226101074452358	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1574824294167214E-4	0.0	0.0	0.028898477987770812	0.0
2	1.1574824294167214E-4	0.0	0.0	0.08565369977683739	0.0
3	1.1574824294167214E-4	0.0	0.0	0.1654042391636495	0.0
4	1.1574824294167214E-4	0.0	0.0	0.28130681309591055	0.0
5	1.1574824294167214E-4	0.0	0.0	0.5000324095080236	0.0
6	1.1574824294167214E-4	0.0	0.0	0.928378073887505	0.0
7	1.1574824294167214E-4	0.0	0.0	1.166896619842644	0.0
8	1.1574824294167214E-4	0.0	0.0	1.679005429364249	0.0
9	1.1574824294167214E-4	0.0	0.0	1.9527886066689508	0.0
10	1.1574824294167214E-4	0.0	0.0	2.303660113772806	0.0
11	1.1574824294167214E-4	0.0	0.0	2.6006315224134897	0.0
12	1.543309905888962E-4	0.0	0.0	2.853464267745749	0.0
13	1.543309905888962E-4	0.0	0.0	2.9866133298763193	0.0
14	1.543309905888962E-4	0.0	0.0	3.066363869263131	0.0
15	1.543309905888962E-4	0.0	0.0	3.136738800971668	0.0
16	1.543309905888962E-4	0.0	0.0	3.2518697199509843	0.0
17	1.543309905888962E-4	0.0	0.0	3.3913077699480523	0.0
18	1.543309905888962E-4	0.0	0.0	3.563155327968788	0.0
19	1.543309905888962E-4	0.0	0.0	3.6802153843304657	0.0
20	1.543309905888962E-4	0.0	0.0	3.800902218970983	0.0
21	1.543309905888962E-4	0.0	0.0	3.964724565481096	0.0
22	1.543309905888962E-4	0.0	0.0	4.13923433308949	0.0
23	1.543309905888962E-4	0.0	0.0	4.334463036184444	0.0
24	1.543309905888962E-4	0.0	0.0	4.479611332833301	0.0
25	1.543309905888962E-4	0.0	0.0	4.610754092086215	0.0
26	1.543309905888962E-4	0.0	0.0	4.733871639828507	0.0
27	1.543309905888962E-4	0.0	0.0	4.86281518246553	0.0
28	1.543309905888962E-4	0.0	0.0	5.000131181342001	0.0
29	1.543309905888962E-4	0.0	0.0	5.162024390469752	0.0
30	1.543309905888962E-4	0.0	0.0	5.3771232086030265	0.0
31	1.9291373823612025E-4	0.0	0.0	5.55344636535084	0.0
32	1.9291373823612025E-4	0.0	0.0	5.733512048620435	0.0
33	1.9291373823612025E-4	0.0	0.0	5.907134413032943	0.0
34	1.9291373823612025E-4	0.0	0.0	6.089862305890197	0.0
35	2.3149648588334428E-4	0.0	0.0	6.299791035838743	0.0
36	2.3149648588334428E-4	0.0	0.0	6.4929362705607465	0.0
37	2.3149648588334428E-4	0.0	0.0	6.691676003691597	0.0
38	2.3149648588334428E-4	0.0	0.0	6.915764602026675	0.0
39	2.3149648588334428E-4	0.0	0.0	7.2002737831773045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCACG	35	1.2127202E-7	45.000004	14
CGTTAAC	20	7.034928E-4	45.0	13
CGTTTTT	18745	0.0	43.667645	1
CGACGGT	220	0.0	41.93182	28
CGCATCG	70	0.0	41.785717	21
ACGGGTA	185	0.0	41.351353	5
TACGGGA	1025	0.0	39.95122	4
ATAGGGA	4540	0.0	38.854626	4
TAGGGAT	5340	0.0	38.679775	5
TAGGGAC	3330	0.0	38.58108	5
TCGCGCG	35	6.2513245E-6	38.57143	1
GGGCGAT	3705	0.0	38.441296	7
CGCATTA	65	9.094947E-12	38.07692	27
GTTAGCG	130	0.0	38.07692	1
CGGTCTA	245	0.0	37.65306	31
TTAGGGA	4190	0.0	37.5895	4
CTACGGG	450	0.0	37.5	3
TCGACGG	150	0.0	37.5	2
AGTCCGC	30	1.1403173E-4	37.499996	11
TCGCGCA	295	0.0	37.372883	34
>>END_MODULE