FastQCFastQC Report
Thu 26 May 2016
SRR1547644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547644_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1340582
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82500.6154043542282381No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61070.4555484110632546No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24190.18044401610643734No Hit
GAGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT18850.14061057063275503No Hit
AAGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT17510.13061491203074485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC900.045.00000416
CGGGTAC253.8911538E-545.0000046
CCTTATG253.8911538E-545.00000427
TCGTTAG253.8911538E-545.0000041
ATTAGAC253.8911538E-545.00000438
ACGTTAG502.1827873E-1145.0000041
CCGGATA502.1827873E-1145.00000420
CCGTGTA253.8911538E-545.00000431
CACGCAT253.8911538E-545.00000433
GTTAGCG1150.045.0000041
TTCCGTA253.8911538E-545.00000428
TGACGTG253.8911538E-545.00000429
TCTAGTT253.8911538E-545.00000428
AGGCGAT453.8562575E-1045.0000046
AGGCGAC253.8911538E-545.00000422
CTGACGT253.8911538E-545.00000428
AACGGTC253.8911538E-545.00000426
CACCTAG253.8911538E-545.0000041
CCCGTAT253.8911538E-545.00000442
AGTGCCG253.8911538E-545.00000415