##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547644_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1340582
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259403751504944	31.0	31.0	33.0	30.0	34.0
2	31.64574565375337	31.0	31.0	34.0	30.0	34.0
3	31.71829250280848	31.0	31.0	34.0	30.0	34.0
4	35.5060212653907	37.0	35.0	37.0	33.0	37.0
5	35.41079471453443	37.0	35.0	37.0	33.0	37.0
6	35.462713209635815	37.0	35.0	37.0	33.0	37.0
7	35.862690234539926	37.0	35.0	37.0	35.0	37.0
8	35.90367318075284	37.0	35.0	37.0	35.0	37.0
9	37.758492207116014	39.0	37.0	39.0	35.0	39.0
10	37.14842732484846	39.0	37.0	39.0	33.0	39.0
11	36.71212055659408	39.0	35.0	39.0	32.0	39.0
12	35.38713782521323	35.0	35.0	39.0	31.0	39.0
13	34.83020658191741	35.0	34.0	39.0	29.0	39.0
14	35.66781890253636	36.0	34.0	40.0	29.0	41.0
15	36.124378068629895	36.0	35.0	40.0	31.0	41.0
16	36.37900404451201	36.0	35.0	40.0	32.0	41.0
17	36.32125077018787	36.0	35.0	40.0	32.0	41.0
18	36.243231671020496	36.0	35.0	40.0	31.0	41.0
19	36.130753657739696	36.0	35.0	40.0	31.0	41.0
20	35.889757582900565	36.0	35.0	40.0	31.0	41.0
21	35.64406653229717	35.0	34.0	40.0	30.0	41.0
22	35.56593479548435	35.0	34.0	40.0	30.0	41.0
23	35.611405344842765	35.0	34.0	40.0	31.0	41.0
24	35.47414704956504	35.0	34.0	40.0	30.0	41.0
25	35.390701948855046	35.0	34.0	40.0	30.0	41.0
26	35.25361223707315	35.0	34.0	40.0	30.0	41.0
27	35.224426405844625	35.0	34.0	40.0	30.0	41.0
28	35.255810536020924	35.0	34.0	40.0	30.0	41.0
29	35.35128101078487	36.0	34.0	40.0	30.0	41.0
30	35.376787096947446	36.0	34.0	40.0	30.0	41.0
31	35.14560392426573	35.0	34.0	40.0	30.0	41.0
32	34.9166265099785	35.0	34.0	40.0	29.0	41.0
33	34.7413593498943	35.0	34.0	40.0	27.0	41.0
34	34.61586385614606	35.0	34.0	40.0	27.0	41.0
35	34.44066756080568	35.0	34.0	40.0	27.0	41.0
36	34.25470131629397	35.0	33.0	40.0	24.0	41.0
37	34.19496606697688	35.0	33.0	40.0	24.0	41.0
38	34.16612635407606	35.0	33.0	40.0	24.0	41.0
39	34.17167319865551	35.0	33.0	40.0	24.0	41.0
40	34.017179105791364	35.0	33.0	40.0	23.0	41.0
41	34.0065687887798	35.0	34.0	40.0	23.0	41.0
42	33.95831288201691	35.0	33.0	40.0	23.0	41.0
43	33.87349225933214	35.0	33.0	40.0	23.0	41.0
44	33.74724037768671	35.0	33.0	40.0	23.0	41.0
45	33.63582608150788	35.0	33.0	39.0	23.0	41.0
46	33.59770980066867	35.0	33.0	39.0	23.0	41.0
47	33.55619275807075	35.0	33.0	39.0	23.0	41.0
48	33.49867147253954	35.0	33.0	39.0	23.0	41.0
49	33.515580546359715	35.0	33.0	39.0	23.0	41.0
50	33.37592030923882	35.0	33.0	39.0	23.0	41.0
51	32.91773125403743	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	9.0
15	27.0
16	102.0
17	305.0
18	761.0
19	1480.0
20	2513.0
21	4134.0
22	6240.0
23	9420.0
24	13577.0
25	19603.0
26	25749.0
27	29341.0
28	30874.0
29	34189.0
30	39894.0
31	47782.0
32	59491.0
33	78156.0
34	150121.0
35	265041.0
36	75197.0
37	86937.0
38	128744.0
39	230749.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.2069817437501	24.378963763499733	29.093259494756758	14.320794997993408
2	29.837936060606513	26.072258168467126	30.793341996237455	13.296463774688904
3	27.739892076724885	24.75797825123715	33.42757101020303	14.074558661834935
4	24.84458242763218	28.250192826697656	31.924865468878444	14.980359276791722
5	22.548788511258543	32.17498071733023	31.54682070921436	13.729410062196868
6	21.224065368623478	39.54349677975685	28.601308983710062	10.63112886790961
7	86.48310957479661	4.0998611051021125	7.855841716508204	1.5611876035930663
8	87.13424467880368	3.0586715322151123	7.146597522568555	2.6604862664126476
9	83.74056939448687	4.883998144089657	8.962674420512881	2.4127580409105893
10	56.67613021806947	22.010887808429473	12.641524352855699	8.671457620645363
11	52.19889570350787	16.745637342587024	20.499156336576206	10.556310617328892
12	48.625298564354885	19.45289433992102	20.991479819958794	10.930327275765302
13	19.536216359760164	48.876532729814365	20.398677589285846	11.188573321139625
14	11.584446158459535	49.011921687744575	25.595748712126525	13.807883441669363
15	9.742037413600958	23.94773314873689	54.22018198066213	12.090047457000018
16	10.873710075176305	18.4024550531038	53.17750051843154	17.546334353288348
17	12.323677328205212	19.380090139954138	28.198648049876844	40.09758448196381
18	20.307672339327247	23.36306171498648	36.78999121277177	19.53927473291451
19	28.07534339562966	26.932854536313332	24.922459051367245	20.069343016689768
20	31.625816249957104	23.335834734466072	26.25061353949255	18.78773547608427
21	18.19754405176259	30.725311842170044	29.82271878930196	21.254425316765406
22	20.8144671493426	24.828470022721476	26.18280716882667	28.174255659109253
23	16.09099629862254	31.686013984970707	24.580070447014805	27.642919269391953
24	18.057679425801627	22.665155880058062	43.542506165232716	15.734658528907595
25	12.611089810246595	26.4464240158379	38.054143648057334	22.88834252585817
26	13.220452012633318	39.51328602054928	27.80538601890821	19.46087594790919
27	14.399715944268982	38.54490064762916	30.61729905369459	16.438084354407266
28	12.094821502899487	30.393217274288332	42.51526575770822	14.996695465103963
29	11.384160014083436	23.711044904377353	42.509596578202604	22.395198503336612
30	16.031842886149448	33.31418742009068	34.18201945125326	16.471950242506615
31	29.82271878930196	27.800239000672843	24.63594170293201	17.741100507093186
32	29.890972726770908	27.94525064486917	26.5843491856522	15.579427442707718
33	27.427117475842582	28.800103238742576	23.356497401874705	20.416281883540133
34	16.524539341867936	29.931402927982028	28.22632259720032	25.31773513294972
35	16.705580113711807	27.332307908057846	32.43994026475068	23.52217171347967
36	30.14601121005653	23.608999673276234	28.548943667750276	17.696045448916962
37	16.101364929560443	33.03162357841594	33.95241768127574	16.914593810747867
38	16.727436292595304	34.768704935617514	24.831155423539926	23.672703348247254
39	17.372230866892142	33.468224994815685	30.607005017223866	18.552539121068314
40	23.786459910695505	27.557657793406147	25.476322970172653	23.179559325725695
41	14.497360101806528	22.793756741474972	31.486622974200756	31.22226018251774
42	22.05116882070623	22.761382742719206	26.468131005787036	28.719317430787523
43	21.85483618309063	24.908882858340633	27.368710008041287	25.86757095052746
44	16.888261963833617	33.501195749308884	27.84096757975267	21.769574707104823
45	14.82490440719031	42.507806310990304	21.37041971322903	21.29686956859036
46	22.410714152509883	35.50346043733244	24.112288543334166	17.973536866823515
47	19.441481386442604	28.770041668469364	27.631804693782254	24.15667225130578
48	22.106517915353184	23.176053385768274	34.017762434524705	20.69966626435384
49	19.631921061151054	22.441447072987703	34.499568098035034	23.427063767826212
50	17.236991097896286	33.85417676800076	29.219697116625465	19.689135017477483
51	15.27090472645463	35.181510716987106	25.46796838984859	24.079616166709684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	911.0
1	1130.5
2	1350.0
3	6537.0
4	11724.0
5	8534.5
6	5345.0
7	5991.0
8	6637.0
9	7576.0
10	8515.0
11	8811.0
12	9107.0
13	9006.0
14	8905.0
15	8356.0
16	7807.0
17	7419.5
18	7032.0
19	6543.5
20	6055.0
21	5764.0
22	5473.0
23	5582.5
24	5692.0
25	6545.5
26	8128.5
27	8858.0
28	10702.5
29	12547.0
30	15163.0
31	17779.0
32	22745.0
33	27711.0
34	31781.5
35	35852.0
36	37929.5
37	40007.0
38	47128.5
39	54250.0
40	80989.0
41	107728.0
42	137021.5
43	166315.0
44	171055.5
45	175796.0
46	166895.5
47	157995.0
48	144679.0
49	131363.0
50	114603.5
51	97844.0
52	83625.0
53	69406.0
54	57910.0
55	46414.0
56	38313.0
57	30212.0
58	25374.0
59	20536.0
60	18633.0
61	16730.0
62	14274.0
63	11818.0
64	9928.0
65	8038.0
66	6434.5
67	4831.0
68	3670.0
69	2509.0
70	1901.0
71	1293.0
72	1323.0
73	1353.0
74	961.0
75	517.5
76	466.0
77	321.5
78	177.0
79	158.0
80	139.0
81	100.5
82	62.0
83	41.5
84	21.0
85	12.5
86	4.0
87	3.5
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1340582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.58874549043267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84255854124854	14.841730000282402
2	8.658720549743448	3.2190950514392047
3	2.9363606118597483	1.6374978024597604
4	1.3963449398082177	1.0382520281179395
5	0.8129938668094557	0.7556268037701844
6	0.5117054105579544	0.5707176985763504
7	0.3779132861010874	0.4917453724950327
8	0.2859370507238185	0.42521688497519994
9	0.2223127793224678	0.3719264106686469
>10	2.2269807670494655	10.045129215336344
>50	1.144085317478373	15.544173565284872
>100	1.5731173112694605	48.31825641381696
>500	0.008125605946639775	1.017362391156007
>1k	0.0020314014866599436	0.6389951148074364
>5k	8.125605946639774E-4	1.0842752468136585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8250	0.6154043542282381	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6107	0.4555484110632546	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2419	0.18044401610643734	No Hit
GAGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	1885	0.14061057063275503	No Hit
AAGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	1751	0.13061491203074485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08227769729863596	0.0
2	0.0	0.0	0.0	0.3250826879668681	0.0
3	0.0	0.0	0.0	0.4950834786682202	0.0
4	0.0	0.0	0.0	1.0296274304742268	0.0
5	0.0	0.0	0.0	1.6253388453671613	0.0
6	0.0	0.0	0.0	2.386799166332235	0.0
7	0.0	0.0	0.0	2.704422407581185	0.0
8	0.0	0.0	0.0	3.6082835663913135	0.0
9	0.0	0.0	0.0	3.85795124804003	0.0
10	0.0	0.0	0.0	4.338637994542669	0.0
11	0.0	0.0	0.0	4.917938626656183	0.0
12	0.0	0.0	0.0	5.380797295502998	0.0
13	0.0	0.0	0.0	5.563777523493528	0.0
14	0.0	0.0	0.0	5.635313617518362	0.0
15	0.0	0.0	0.0	5.74735450722149	0.0
16	0.0	0.0	0.0	6.010300004028101	0.0
17	0.0	0.0	0.0	6.293087629104374	0.0
18	0.0	0.0	0.0	6.68291831458277	0.0
19	0.0	0.0	0.0	6.860303957534862	0.0
20	0.0	0.0	0.0	7.044999858270512	0.0
21	0.0	0.0	0.0	7.313092373312487	0.0
22	0.0	0.0	0.0	7.551869262753043	0.0
23	0.0	0.0	0.0	7.836745532910333	0.0
24	0.0	0.0	0.0	8.03986626703924	0.0
25	0.0	0.0	0.0	8.206361117783171	0.0
26	0.0	0.0	0.0	8.374422452337866	0.0
27	0.0	0.0	0.0	8.527341110055184	0.0
28	0.0	0.0	0.0	8.686376514081198	0.0
29	0.0	0.0	0.0	8.873832410102478	0.0
30	0.0	0.0	0.0	9.110446059994839	0.0
31	0.0	0.0	0.0	9.34512025374054	0.0
32	0.0	0.0	0.0	9.525489675379797	0.0
33	0.0	0.0	0.0	9.704367207675473	0.0
34	0.0	0.0	0.0	9.904131190781317	0.0
35	0.0	0.0	0.0	10.168568576931511	0.0
36	0.0	0.0	0.0	10.37526984548502	0.0
37	0.0	0.0	0.0	10.582344086374425	0.0
38	0.0	0.0	0.0	10.770471332600318	0.0
39	0.0	0.0	0.0	10.97873908496459	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	90	0.0	45.000004	16
CGGGTAC	25	3.8911538E-5	45.000004	6
CCTTATG	25	3.8911538E-5	45.000004	27
TCGTTAG	25	3.8911538E-5	45.000004	1
ATTAGAC	25	3.8911538E-5	45.000004	38
ACGTTAG	50	2.1827873E-11	45.000004	1
CCGGATA	50	2.1827873E-11	45.000004	20
CCGTGTA	25	3.8911538E-5	45.000004	31
CACGCAT	25	3.8911538E-5	45.000004	33
GTTAGCG	115	0.0	45.000004	1
TTCCGTA	25	3.8911538E-5	45.000004	28
TGACGTG	25	3.8911538E-5	45.000004	29
TCTAGTT	25	3.8911538E-5	45.000004	28
AGGCGAT	45	3.8562575E-10	45.000004	6
AGGCGAC	25	3.8911538E-5	45.000004	22
CTGACGT	25	3.8911538E-5	45.000004	28
AACGGTC	25	3.8911538E-5	45.000004	26
CACCTAG	25	3.8911538E-5	45.000004	1
CCCGTAT	25	3.8911538E-5	45.000004	42
AGTGCCG	25	3.8911538E-5	45.000004	15
>>END_MODULE