FastQCFastQC Report
Thu 26 May 2016
SRR1547643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547643_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1501924
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.48238126562995204No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24710.16452230605543292No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16330.10872720590389394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG406.8175723E-945.01
GTTACGC207.0339796E-445.037
CGACGGT1800.041.2528
CGTTTTT46150.040.1733471
CACGACG1850.040.13513626
GACGGTC1850.040.13513629
ACGGGAC4950.040.05
GGGCGTA403.4591721E-739.3757
TCACGAC1900.039.07894525
TACGGGA4450.038.4269644
GGCGATA5100.038.3823558
TCGTCCC8050.038.29192738
GGGCGAT21900.038.1164367
GCTACGA3900.038.07692310
TAGGGAC13900.038.039575
CTACGGG2550.037.9411773
TATGGGA18300.037.7459034
AACCCGC601.5643309E-1037.49999621
CGTGATA301.14008886E-437.49999643
CGCGACG301.14008886E-437.49999615