##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547643_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1501924
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345480197400136	31.0	31.0	33.0	30.0	34.0
2	31.75860163363792	31.0	31.0	34.0	30.0	34.0
3	31.90236789611192	31.0	31.0	34.0	30.0	34.0
4	35.67204066251022	37.0	35.0	37.0	33.0	37.0
5	35.58803508033695	37.0	35.0	37.0	33.0	37.0
6	35.624753316412814	37.0	35.0	37.0	33.0	37.0
7	35.91787866762899	37.0	35.0	37.0	35.0	37.0
8	35.93149054146548	37.0	35.0	37.0	35.0	37.0
9	37.74115401311917	39.0	37.0	39.0	35.0	39.0
10	37.17817812352689	39.0	37.0	39.0	33.0	39.0
11	36.89933844854999	39.0	37.0	39.0	33.0	39.0
12	36.350756762659096	38.0	35.0	39.0	32.0	39.0
13	36.162652038318846	38.0	35.0	39.0	32.0	39.0
14	37.19260894692408	39.0	35.0	41.0	32.0	41.0
15	37.395221063116374	39.0	35.0	41.0	32.0	41.0
16	37.48463570726614	39.0	35.0	41.0	33.0	41.0
17	37.39134470186241	39.0	35.0	41.0	32.0	41.0
18	37.299484527845614	39.0	35.0	41.0	32.0	41.0
19	37.270435787696314	39.0	35.0	41.0	32.0	41.0
20	37.12802711721765	39.0	35.0	41.0	32.0	41.0
21	36.94697268303855	39.0	35.0	41.0	32.0	41.0
22	36.88528514092591	39.0	35.0	41.0	31.0	41.0
23	36.827192321315856	38.0	35.0	41.0	31.0	41.0
24	36.74125455082947	38.0	35.0	41.0	31.0	41.0
25	36.63616334781254	38.0	35.0	40.0	31.0	41.0
26	36.52952080131884	38.0	35.0	40.0	31.0	41.0
27	36.44843281018214	38.0	35.0	40.0	31.0	41.0
28	36.46234762877482	38.0	35.0	40.0	31.0	41.0
29	36.466989008764756	38.0	35.0	40.0	31.0	41.0
30	36.47881517307134	38.0	35.0	40.0	31.0	41.0
31	36.414715391724215	38.0	35.0	41.0	30.0	41.0
32	36.3010438610742	38.0	35.0	41.0	30.0	41.0
33	36.18907081849681	38.0	35.0	41.0	30.0	41.0
34	36.093763066573274	38.0	35.0	41.0	30.0	41.0
35	35.92782191375862	38.0	35.0	41.0	29.0	41.0
36	35.829722409389554	38.0	35.0	41.0	29.0	41.0
37	35.80242808557557	38.0	35.0	41.0	29.0	41.0
38	35.715311160884305	38.0	35.0	40.0	29.0	41.0
39	35.66078310220757	38.0	35.0	40.0	29.0	41.0
40	35.58380983325388	38.0	35.0	40.0	28.0	41.0
41	35.49789936108618	38.0	34.0	40.0	28.0	41.0
42	35.46784990452247	38.0	34.0	40.0	27.0	41.0
43	35.42727461575952	38.0	34.0	40.0	27.0	41.0
44	35.29270322599545	38.0	34.0	40.0	27.0	41.0
45	35.197539289604535	38.0	34.0	40.0	27.0	41.0
46	35.16793326426637	37.0	34.0	40.0	27.0	41.0
47	35.132219739480824	37.0	34.0	40.0	27.0	41.0
48	35.015452845816434	37.0	34.0	40.0	26.0	41.0
49	34.956648938295146	37.0	34.0	40.0	26.0	41.0
50	34.866918698948815	37.0	34.0	40.0	26.0	41.0
51	34.443036398646	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	5.0
14	22.0
15	34.0
16	77.0
17	220.0
18	512.0
19	1054.0
20	1757.0
21	3011.0
22	4589.0
23	6928.0
24	10326.0
25	14896.0
26	19898.0
27	23325.0
28	25808.0
29	29822.0
30	34985.0
31	43519.0
32	53986.0
33	70862.0
34	117173.0
35	175505.0
36	108699.0
37	140553.0
38	216706.0
39	397507.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.688522188872405	24.17951907020595	28.62601569719906	13.505943043722585
2	31.30584503610036	26.119697135141323	29.157800261531207	13.416657567227103
3	29.301748956671574	24.82921905502542	31.918326093730442	13.950705894572563
4	25.69990225870284	28.482865977239864	30.974070592120505	14.843161171936794
5	23.151304593308318	32.82090172338947	29.686255762608493	14.341537920693723
6	22.323166818028074	40.36036443921264	27.43960413443024	9.87686460832905
7	88.19327742282566	3.6360028869636545	6.432549183580527	1.7381705066301625
8	88.20093426831185	3.361288587172187	6.468769391793459	1.969007752722508
9	82.69526287615085	6.182270208079769	8.195554502091984	2.92691241367739
10	42.38809686775096	32.45397237143823	14.36011409365587	10.79781666715493
11	34.52511578482	24.033972424703247	26.349868568582696	15.09104322189405
12	34.32111078856187	22.51179154204873	26.946836191445104	16.22026147794429
13	23.202838492493626	31.997824124256617	27.389801348137453	17.409536035112296
14	18.307584138744705	33.78100356609256	29.39556195919367	18.515850335969063
15	17.16172056642014	27.151706744149507	38.90915918515184	16.777413504278513
16	20.22652278011404	24.88028688535505	37.244094907598516	17.649095426932387
17	20.18757274003212	24.652645539987375	29.396028028049354	25.76375369193115
18	22.645486722364115	25.946585845888343	32.438458936670564	18.96946849507698
19	25.759758816025315	27.944023798807393	26.850626263379507	19.445591121787785
20	27.414369834958357	26.536895342241017	28.76989781107433	17.278837011726292
21	23.728297836641534	27.64314306183269	30.069231199448176	18.5593279020776
22	23.475089285476493	25.245351961883557	28.661902999086504	22.617655753553443
23	21.031423693875322	28.14183673741148	28.775623799872697	22.0511157688405
24	20.505032212016054	25.465070136704654	35.491276522646956	18.53862112863234
25	19.611644796940457	28.389119555982862	32.053685805673254	19.945549841403427
26	18.92133024041163	32.54052801606473	27.720177585550264	20.817964157973375
27	18.11616300158996	32.71217451748557	30.36445252888961	18.807209952034857
28	17.389761399378397	29.266660629965298	34.84623722638429	18.497340744272016
29	18.315307565495992	26.190073532349174	34.62711828294907	20.867500619205764
30	19.544397719192183	30.194736884156587	31.551729648104697	18.709135748546533
31	24.947800288163716	28.46329108530125	27.534881924784475	19.054026701750555
32	25.337700176573513	27.651665463765145	28.35682764241067	18.653806717250674
33	24.240574090300175	28.670025913428375	27.373755263249006	19.715644733022444
34	19.43214170623813	28.274000548629623	29.964432288185023	22.32942545694722
35	20.154548432543855	28.05168570446973	30.649020855915477	21.14474500707093
36	25.00765684548619	27.670907449378262	28.36142174970238	18.960013955433162
37	20.63413328503972	30.992513602552457	29.709359461597256	18.66399365081056
38	19.893949360953016	31.380615796804634	27.80147331023407	20.923961532008278
39	19.87610558190694	30.496216852517172	29.380914080872266	20.24676348470362
40	23.079263664473036	27.553857585337205	28.389918531164028	20.97696021902573
41	18.86140710182406	25.94651926462324	30.769200039416113	24.422873594136586
42	21.211792341023912	26.490022131612516	27.824177521632254	24.474008005731314
43	21.109590099099552	27.339199586663504	28.26794165350577	23.283268660731167
44	19.599726750488042	29.760360710661793	29.135162631398124	21.50474990745204
45	18.650411072730712	33.27578492653423	26.932987288304865	21.140816712430187
46	21.07450177239328	31.427422426168032	27.358707897337016	20.13936790410167
47	19.792612675474924	28.16727078067865	30.236083849782013	21.80403269406441
48	21.080227761191644	27.2554403551711	31.72983453223998	19.934497351397273
49	20.816699113936522	27.423158561951205	30.502808397761804	21.25733392635047
50	19.58214929650235	30.609937653303366	29.923285066354886	19.884627983839394
51	18.625509679584322	30.876795363813347	28.193037730271303	22.304657226331027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	797.0
1	1492.0
2	2187.0
3	7472.0
4	12757.0
5	9298.5
6	5840.0
7	5874.0
8	5908.0
9	6280.5
10	6653.0
11	6716.5
12	6780.0
13	6516.5
14	6253.0
15	6274.5
16	6296.0
17	6216.5
18	6137.0
19	6257.0
20	6377.0
21	7097.0
22	7817.0
23	8553.0
24	9289.0
25	11088.0
26	15403.0
27	17919.0
28	22280.0
29	26641.0
30	31155.0
31	35669.0
32	40361.0
33	45053.0
34	52646.0
35	60239.0
36	63954.0
37	67669.0
38	73563.0
39	79457.0
40	91834.0
41	104211.0
42	115789.0
43	127367.0
44	128917.5
45	130468.0
46	126961.0
47	123454.0
48	120279.5
49	117105.0
50	109617.5
51	102130.0
52	93650.5
53	85171.0
54	77274.0
55	69377.0
56	61086.0
57	52795.0
58	48158.5
59	43522.0
60	38569.0
61	33616.0
62	29949.0
63	26282.0
64	22769.5
65	19257.0
66	16708.0
67	14159.0
68	11947.0
69	9735.0
70	7673.5
71	5612.0
72	4929.0
73	4246.0
74	3306.5
75	1812.5
76	1258.0
77	917.0
78	576.0
79	489.5
80	403.0
81	248.5
82	94.0
83	72.5
84	51.0
85	34.0
86	17.0
87	11.5
88	6.0
89	5.0
90	4.0
91	9.0
92	14.0
93	7.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1501924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.565209688235154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.21016247499749	15.106330188860309
2	6.594159287934091	2.580322183721078
3	2.332330354990268	1.3689759737286158
4	1.285651510416868	1.0061616554920905
5	0.8903515510253139	0.8709957396027833
6	0.6814602756992002	0.7999747912954432
7	0.525555866376097	0.7197827509971302
8	0.4546685645020535	0.7116548642505238
9	0.4118277895429387	0.7251747352064973
>10	6.888391715232337	33.73198967020451
>50	2.3565322044499237	32.64031383357533
>100	0.3678740822030225	8.972767771076468
>500	0.0	0.0
>1k	6.895484202488529E-4	0.2768385915520293
>5k	3.4477421012442643E-4	0.4887172504372446
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7245	0.48238126562995204	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2471	0.16452230605543292	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1633	0.10872720590389394	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024568486820904387	0.0
2	0.0	0.0	0.0	0.0786990553450108	0.0
3	0.0	0.0	0.0	0.12623807862448433	0.0
4	0.0	0.0	0.0	0.22411253831751807	0.0
5	0.0	0.0	0.0	0.38184355533302616	0.0
6	0.0	0.0	0.0	0.6094849007007013	0.0
7	0.0	0.0	0.0	0.7237383516076712	0.0
8	0.0	0.0	0.0	1.0051773591739661	0.0
9	0.0	0.0	0.0	1.11563567797039	0.0
10	0.0	0.0	0.0	1.3185087927218688	0.0
11	0.0	0.0	0.0	1.5444856064621113	0.0
12	0.0	0.0	0.0	1.7383702504254543	0.0
13	0.0	0.0	0.0	1.8213305067366925	0.0
14	0.0	0.0	0.0	1.8566185772382624	0.0
15	0.0	0.0	0.0	1.9126800024501907	0.0
16	0.0	0.0	0.0	2.02966328522615	0.0
17	0.0	0.0	0.0	2.1664212037360078	0.0
18	0.0	0.0	0.0	2.327481284006381	0.0
19	0.0	0.0	0.0	2.421627192853966	0.0
20	0.0	0.0	0.0	2.5289561921908166	0.0
21	0.0	0.0	0.0	2.667112317267718	0.0
22	0.0	0.0	0.0	2.8163209323507714	0.0
23	0.0	0.0	0.0	2.9793784505740635	0.0
24	0.0	0.0	0.0	3.1059494355240345	0.0
25	0.0	0.0	0.0	3.218804679863961	0.0
26	0.0	0.0	0.0	3.332192574324666	0.0
27	0.0	0.0	0.0	3.4494421821610146	0.0
28	6.658126509730186E-5	0.0	0.0	3.5613652887895793	0.0
29	6.658126509730186E-5	0.0	0.0	3.693196193682237	0.0
30	6.658126509730186E-5	0.0	0.0	3.8668401330560003	0.0
31	6.658126509730186E-5	0.0	0.0	4.017380373441	0.0
32	6.658126509730186E-5	0.0	0.0	4.162727275148409	0.0
33	6.658126509730186E-5	0.0	0.0	4.308007595590722	0.0
34	6.658126509730186E-5	0.0	0.0	4.471398020139501	0.0
35	6.658126509730186E-5	0.0	0.0	4.655095730542957	0.0
36	6.658126509730186E-5	0.0	0.0	4.819817780393682	0.0
37	6.658126509730186E-5	0.0	0.0	4.986004618076547	0.0
38	6.658126509730186E-5	0.0	0.0	5.170368141130976	0.0
39	6.658126509730186E-5	0.0	0.0	5.427704730732048	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	40	6.8175723E-9	45.0	1
GTTACGC	20	7.0339796E-4	45.0	37
CGACGGT	180	0.0	41.25	28
CGTTTTT	4615	0.0	40.173347	1
CACGACG	185	0.0	40.135136	26
GACGGTC	185	0.0	40.135136	29
ACGGGAC	495	0.0	40.0	5
GGGCGTA	40	3.4591721E-7	39.375	7
TCACGAC	190	0.0	39.078945	25
TACGGGA	445	0.0	38.426964	4
GGCGATA	510	0.0	38.382355	8
TCGTCCC	805	0.0	38.291927	38
GGGCGAT	2190	0.0	38.116436	7
GCTACGA	390	0.0	38.076923	10
TAGGGAC	1390	0.0	38.03957	5
CTACGGG	255	0.0	37.941177	3
TATGGGA	1830	0.0	37.745903	4
AACCCGC	60	1.5643309E-10	37.499996	21
CGTGATA	30	1.14008886E-4	37.499996	43
CGCGACG	30	1.14008886E-4	37.499996	15
>>END_MODULE