FastQCFastQC Report
Thu 26 May 2016
SRR1547642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547642_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1996522
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116090.5814611609589075No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57660.2888022270728797No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20730.103830561346181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCG207.0345367E-445.00000426
CCGTAGA207.0345367E-445.00000432
TCGCTAA302.166309E-645.00000443
TCCCGTA207.0345367E-445.00000430
GTCGGTC207.0345367E-445.00000425
CGTATCA253.891843E-545.018
CACGCGT253.891843E-545.026
ACCTCGT253.891843E-545.014
ACCCGTA253.891843E-545.033
TATCGTG253.891843E-545.01
TCGGTTA351.2125383E-745.038
AGTCGAT253.891843E-545.021
CGTTTTT87900.043.592151
CGACGGT1800.042.50000428
TACGGGA6600.042.2727284
TATAGCG700.041.7857131
TAGCGCG603.6379788E-1241.2500041
TCGTTGA2850.041.05262824
AATACGG2550.040.5882342
CTAACGG1450.040.344832