##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547642_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1996522
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2921705846467	31.0	31.0	33.0	30.0	34.0
2	31.68396140889006	31.0	31.0	34.0	30.0	34.0
3	31.762628210457986	31.0	31.0	34.0	30.0	34.0
4	35.56039302346781	37.0	35.0	37.0	33.0	37.0
5	35.49152726591543	37.0	35.0	37.0	33.0	37.0
6	35.55374646510281	37.0	35.0	37.0	33.0	37.0
7	35.91583663991682	37.0	35.0	37.0	35.0	37.0
8	35.952563007069294	37.0	35.0	37.0	35.0	37.0
9	37.803918013425346	39.0	38.0	39.0	35.0	39.0
10	37.104700574298704	39.0	37.0	39.0	33.0	39.0
11	36.79679011801523	39.0	35.0	39.0	32.0	39.0
12	36.022530680854004	37.0	35.0	39.0	32.0	39.0
13	35.7299543906854	37.0	35.0	39.0	31.0	39.0
14	36.7106312878095	38.0	35.0	41.0	31.0	41.0
15	36.99994991289853	38.0	35.0	41.0	32.0	41.0
16	37.1435100640013	38.0	35.0	41.0	32.0	41.0
17	37.06029284926487	38.0	35.0	41.0	32.0	41.0
18	36.968755666103355	38.0	35.0	40.0	32.0	41.0
19	36.899092521895575	38.0	35.0	40.0	32.0	41.0
20	36.68968386023295	38.0	35.0	40.0	32.0	41.0
21	36.513157881556026	38.0	35.0	40.0	31.0	41.0
22	36.43646050481788	38.0	35.0	40.0	31.0	41.0
23	36.40134393710663	38.0	35.0	40.0	31.0	41.0
24	36.311876352977826	37.0	35.0	40.0	31.0	41.0
25	36.23278581453147	37.0	35.0	40.0	31.0	41.0
26	36.11646853878895	37.0	35.0	40.0	31.0	41.0
27	36.06910567476842	37.0	35.0	40.0	31.0	41.0
28	36.1211566914865	37.0	35.0	40.0	31.0	41.0
29	36.16585792693494	37.0	35.0	40.0	31.0	41.0
30	36.18039771162051	37.0	35.0	40.0	31.0	41.0
31	36.044594549922316	37.0	35.0	40.0	30.0	41.0
32	35.835853048451256	37.0	35.0	40.0	30.0	41.0
33	35.67713052999166	37.0	35.0	40.0	30.0	41.0
34	35.53231619786809	37.0	35.0	40.0	29.0	41.0
35	35.32449679993509	37.0	34.0	40.0	29.0	41.0
36	35.16478005251132	37.0	34.0	40.0	27.0	41.0
37	35.13720960750746	37.0	34.0	40.0	27.0	41.0
38	35.063338145034216	37.0	34.0	40.0	27.0	41.0
39	35.04868015478918	37.0	34.0	40.0	27.0	41.0
40	34.94705743287577	37.0	34.0	40.0	26.0	41.0
41	34.87915635289769	37.0	34.0	40.0	26.0	41.0
42	34.83691289151835	37.0	34.0	40.0	26.0	41.0
43	34.785917710899255	37.0	34.0	40.0	26.0	41.0
44	34.676960734717674	36.0	34.0	40.0	25.0	41.0
45	34.59113247938164	36.0	34.0	40.0	25.0	41.0
46	34.53103246545743	36.0	34.0	40.0	25.0	41.0
47	34.46457239138862	36.0	34.0	40.0	24.0	41.0
48	34.34795709739237	36.0	34.0	40.0	24.0	41.0
49	34.31373909228148	36.0	34.0	40.0	24.0	41.0
50	34.21403270287029	36.0	34.0	40.0	24.0	41.0
51	33.80740908439777	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	4.0
14	12.0
15	43.0
16	101.0
17	251.0
18	630.0
19	1341.0
20	2394.0
21	4134.0
22	6663.0
23	10471.0
24	15937.0
25	23817.0
26	31425.0
27	35740.0
28	37465.0
29	42169.0
30	50220.0
31	62194.0
32	78526.0
33	103832.0
34	182052.0
35	302783.0
36	138746.0
37	171054.0
38	252830.0
39	441520.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.730440235569656	24.95529726193851	30.372868418179216	13.94139408431262
2	30.82380259270872	25.990747910616562	30.635675439589445	12.549774057085273
3	27.651536021140764	25.023616068342847	33.79552040999298	13.529327500523411
4	23.53653002571472	27.920553843133213	33.95144155686739	14.591474574284682
5	21.768254995437065	32.21462122631256	32.17940999397953	13.837713784270846
6	20.67074642803836	39.99720513973801	29.014255790820236	10.3177926414034
7	87.6270334111019	3.2918745698770158	7.552083072463013	1.5290089465580645
8	88.17759083045415	2.5534404329128355	7.03373165935562	2.2352370772773855
9	84.340718509488	4.2728304521563	8.9641386370899	2.4223124012658013
10	46.178253983677614	26.252603277098874	15.944227010771733	11.624915728451777
11	39.24725096943585	21.671837325108363	25.1096156215659	13.971296083889884
12	38.62251455280733	20.437390622292163	25.93114425986791	15.008950565032592
13	20.621661068598293	37.98605775443496	26.54260759460702	14.849673582359724
14	14.873064258745961	39.51271260722396	28.625980580229015	16.98824255380106
15	13.079244806718885	26.724223424535264	44.92332165636041	15.273210112385438
16	14.674518988521038	21.62069839450805	44.884604326924524	18.820178290046393
17	15.428830736651037	24.119844409427994	29.139473544493878	31.31185130942709
18	19.63234064037361	25.85285812027115	34.59471020103961	19.920091038315633
19	24.729855218224493	29.286779709915546	26.232468262308156	19.75089680955181
20	26.953371913758023	24.61435436223593	29.343628570083375	19.08864515392267
21	19.481227855240263	30.53615236896964	30.448750376905437	19.533869398884658
22	21.357290327880182	25.570166519577548	27.506734210792565	25.5658089417497
23	17.66687269161071	30.936147961304712	26.837971231972404	24.559008115112178
24	18.19674413805608	25.405279781540095	39.39175225717523	17.006223823228595
25	15.501857730593501	28.631940945303885	34.60943580887163	21.256765515230985
26	15.358057662274696	35.69021528437953	28.80519222928673	20.14653482405904
27	15.86989775219106	36.20015206444006	30.104802251114688	17.82514793225419
28	14.466206733509573	30.060625427618632	38.29940266122788	17.173765177643922
29	13.814874066000776	24.690937540382727	38.81184379636187	22.682344597254627
30	16.51281578665299	32.655137283736416	32.97123698111015	17.860809948500442
31	25.05021231922313	29.46644214288648	27.138844450499416	18.344501087390974
32	25.82225490127331	28.099515056683572	27.808909693957794	18.269320348085323
33	24.169480726984226	29.46529013955268	26.265525749277995	20.099703384185098
34	18.035263322918553	28.71233074316236	29.243504454245933	24.00890147967315
35	18.451336874825323	26.926525227370394	31.876984075306957	22.745153822497322
36	26.33464594930584	26.538700800692403	29.216808029162717	17.90984522083904
37	18.253342562716565	31.78001544686209	32.33848662824652	17.628155362174823
38	17.76819889788342	31.33333867595749	28.775340316810933	22.123122109348156
39	18.26120623764727	31.38753291974744	30.320226874534818	20.031033968070474
40	22.878435599507544	28.266505452982738	27.63059961272653	21.224459334783187
41	16.39476048848948	24.945630451354905	32.92976486109344	25.729844199062168
42	20.43248208634816	25.789197414303473	28.744636923610155	25.03368357573821
43	20.34663279442951	27.56628777443975	28.243765908915602	23.84331352221513
44	18.004710191022188	31.159185824148196	27.99889006983144	22.83721391499818
45	16.31316860019574	36.770343627568344	24.702056876908944	22.214430895326974
46	21.293178837999278	33.96481481295974	26.186087606347435	18.555918742693546
47	19.036905178104725	28.625379535011387	29.92724347640547	22.410471810478423
48	20.349287410807392	25.948774919585162	33.526252152493186	20.17568551711426
49	19.744936444476945	25.825410388665894	31.85113913094872	22.578514035908444
50	18.00611262986333	31.076592193825064	31.21934043301301	19.697954743298595
51	16.824758254604756	32.128922195698316	27.094717714104828	23.951601835592097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1087.0
1	1610.0
2	2133.0
3	10407.5
4	18682.0
5	13922.5
6	9163.0
7	9499.5
8	9836.0
9	10663.5
10	11491.0
11	11940.5
12	12390.0
13	12173.5
14	11957.0
15	11708.5
16	11460.0
17	10959.5
18	10459.0
19	10242.0
20	10025.0
21	9908.0
22	9791.0
23	10398.5
24	11006.0
25	12857.5
26	18374.0
27	22039.0
28	27160.5
29	32282.0
30	36164.0
31	40046.0
32	46761.5
33	53477.0
34	62481.5
35	71486.0
36	74289.5
37	77093.0
38	86477.5
39	95862.0
40	124046.0
41	152230.0
42	179249.0
43	206268.0
44	209953.0
45	213638.0
46	209890.5
47	206143.0
48	197286.5
49	188430.0
50	169633.0
51	150836.0
52	130859.5
53	110883.0
54	92585.5
55	74288.0
56	62864.0
57	51440.0
58	44333.5
59	37227.0
60	30683.0
61	24139.0
62	20730.5
63	17322.0
64	13869.5
65	10417.0
66	8478.5
67	6540.0
68	5408.5
69	4277.0
70	3370.5
71	2464.0
72	2076.5
73	1689.0
74	1145.5
75	584.0
76	566.0
77	485.5
78	405.0
79	279.5
80	154.0
81	101.0
82	48.0
83	32.5
84	17.0
85	17.5
86	18.0
87	10.5
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1996522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.546660785044356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53451375875451	18.25678894610747
2	9.64773726463589	4.54343993427226
3	3.393881267282487	2.3974371283625158
4	1.718314131368028	1.618422398938844
5	1.0171104824773145	1.1974777755903063
6	0.6985438092045901	0.9869024471299938
7	0.5024745985672526	0.8282119247895103
8	0.3819266421941496	0.7194477670813321
9	0.3056501199120265	0.6477335723228957
>10	2.8503785616621844	15.069350062528377
>50	0.957829464190625	16.36640174572306
>100	0.9849469243297795	35.27264827297332
>500	0.004749853524682287	0.7392326462643839
>1k	0.001511317030580728	0.47319836816098515
>5k	2.1590243294010395E-4	0.2931308327047959
>10k+	2.1590243294010395E-4	0.5901761770499437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11609	0.5814611609589075	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5766	0.2888022270728797	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2073	0.103830561346181	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.008710146945538E-5	0.0	0.0	0.06471253509853636	0.0
2	5.008710146945538E-5	0.0	0.0	0.23345597994913153	0.0
3	5.008710146945538E-5	0.0	0.0	0.39989541813213175	0.0
4	5.008710146945538E-5	0.0	0.0	0.7490025153742358	0.0
5	5.008710146945538E-5	0.0	0.0	1.2955028795074635	0.0
6	5.008710146945538E-5	0.0	0.0	2.0424017366199823	0.0
7	5.008710146945538E-5	0.0	0.0	2.375330700087452	0.0
8	5.008710146945538E-5	0.0	0.0	3.2986864156768623	0.0
9	5.008710146945538E-5	0.0	0.0	3.6279590207370616	0.0
10	5.008710146945538E-5	0.0	0.0	4.262762944760939	0.0
11	5.008710146945538E-5	0.0	0.0	5.015171383035098	0.0
12	5.008710146945538E-5	0.0	0.0	5.583459636307539	0.0
13	5.008710146945538E-5	0.0	0.0	5.8401059442370284	0.0
14	5.008710146945538E-5	0.0	0.0	5.941983108625901	0.0
15	5.008710146945538E-5	0.0	0.0	6.078019676216941	0.0
16	5.008710146945538E-5	0.0	0.0	6.4251232894002674	0.0
17	5.008710146945538E-5	0.0	0.0	6.844903286815772	0.0
18	5.008710146945538E-5	0.0	0.0	7.339813936435461	0.0
19	5.008710146945538E-5	0.0	0.0	7.62500989220254	0.0
20	5.008710146945538E-5	0.0	0.0	7.915164471015095	0.0
21	5.008710146945538E-5	0.0	0.0	8.337098213793787	0.0
22	5.008710146945538E-5	0.0	0.0	8.762337705269463	0.0
23	5.008710146945538E-5	0.0	0.0	9.284345476784127	0.0
24	5.008710146945538E-5	0.0	0.0	9.644972607364206	0.0
25	5.008710146945538E-5	0.0	0.0	9.973093209090608	0.0
26	5.008710146945538E-5	0.0	0.0	10.264950749353124	0.0
27	5.008710146945538E-5	0.0	0.0	10.55420376033923	0.0
28	5.008710146945538E-5	0.0	0.0	10.873158422496722	0.0
29	5.008710146945538E-5	0.0	0.0	11.202831724368677	0.0
30	5.008710146945538E-5	0.0	0.0	11.590405715539323	0.0
31	5.008710146945538E-5	0.0	0.0	11.965858628154361	0.0
32	5.008710146945538E-5	0.0	0.0	12.288669997125	0.0
33	5.008710146945538E-5	0.0	0.0	12.627659499870274	0.0
34	5.008710146945538E-5	0.0	0.0	12.960738724642153	0.0
35	5.008710146945538E-5	0.0	0.0	13.360333620165468	0.0
36	5.008710146945538E-5	0.0	0.0	13.711193765959003	0.0
37	5.008710146945538E-5	0.0	0.0	14.073223335380225	0.0
38	5.008710146945538E-5	0.0	0.0	14.420226774360613	0.0
39	5.008710146945538E-5	0.0	0.0	14.76262220000581	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACCG	20	7.0345367E-4	45.000004	26
CCGTAGA	20	7.0345367E-4	45.000004	32
TCGCTAA	30	2.166309E-6	45.000004	43
TCCCGTA	20	7.0345367E-4	45.000004	30
GTCGGTC	20	7.0345367E-4	45.000004	25
CGTATCA	25	3.891843E-5	45.0	18
CACGCGT	25	3.891843E-5	45.0	26
ACCTCGT	25	3.891843E-5	45.0	14
ACCCGTA	25	3.891843E-5	45.0	33
TATCGTG	25	3.891843E-5	45.0	1
TCGGTTA	35	1.2125383E-7	45.0	38
AGTCGAT	25	3.891843E-5	45.0	21
CGTTTTT	8790	0.0	43.59215	1
CGACGGT	180	0.0	42.500004	28
TACGGGA	660	0.0	42.272728	4
TATAGCG	70	0.0	41.785713	1
TAGCGCG	60	3.6379788E-12	41.250004	1
TCGTTGA	285	0.0	41.052628	24
AATACGG	255	0.0	40.588234	2
CTAACGG	145	0.0	40.34483	2
>>END_MODULE