FastQCFastQC Report
Thu 26 May 2016
SRR1547641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547641_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1627766
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353302.170459390354633No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT23340.14338670300276576No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCT17510.1075707441978761No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16490.10130448725431052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT164300.043.466221
CCGAACC556.184564E-1140.90909218
GCGATAC501.0822987E-940.59
TAACGCG451.9292202E-840.0000041
TACGCGG800.039.3752
TCACGAC1100.038.86363625
AATCGGT356.249893E-638.57142626
GGGCGAT22000.038.1477287
TAGGGAC15400.037.6948055
GTTTTTT194350.037.6369972
CGGGACC3650.037.6027376
AAGGGCG4850.037.577325
TTACGCG301.1401305E-437.5000041
TAATGCG601.5643309E-1037.5000041
AGGGATC15850.037.476346
GGCACCG4950.037.2727288
GACCGAT7500.037.29
TATGGGC6600.037.1590924
ATATGCG850.037.0588231
CTCGTAG552.750312E-936.818181
Produced by FastQC (version 0.11.5)