##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547641_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1627766
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30156914445934	31.0	31.0	33.0	30.0	34.0
2	31.693237234344494	31.0	31.0	34.0	30.0	34.0
3	31.607209512915247	31.0	31.0	34.0	30.0	34.0
4	35.53172384728518	37.0	35.0	37.0	33.0	37.0
5	35.50032989999791	37.0	35.0	37.0	33.0	37.0
6	35.56275349159523	37.0	35.0	37.0	33.0	37.0
7	35.91341814486849	37.0	35.0	37.0	35.0	37.0
8	35.943061840583965	37.0	35.0	37.0	35.0	37.0
9	37.75458819019441	39.0	37.0	39.0	35.0	39.0
10	37.15992716397812	39.0	37.0	39.0	33.0	39.0
11	36.89209566977072	39.0	37.0	39.0	33.0	39.0
12	36.43624513597163	39.0	35.0	39.0	32.0	39.0
13	36.293757825141945	39.0	35.0	39.0	32.0	39.0
14	37.36965325482901	39.0	35.0	41.0	32.0	41.0
15	37.54119203865912	40.0	35.0	41.0	32.0	41.0
16	37.59141854541746	40.0	35.0	41.0	33.0	41.0
17	37.48393995205699	39.0	35.0	41.0	32.0	41.0
18	37.278434369559264	39.0	36.0	41.0	32.0	41.0
19	37.11626363985978	38.0	35.0	41.0	32.0	41.0
20	36.870638040111416	38.0	35.0	41.0	32.0	41.0
21	36.71110282436173	38.0	35.0	41.0	31.0	41.0
22	36.6631905323001	38.0	35.0	40.0	31.0	41.0
23	36.59035573909272	38.0	35.0	40.0	31.0	41.0
24	36.467545089404744	38.0	35.0	40.0	31.0	41.0
25	36.348805663713335	38.0	35.0	40.0	31.0	41.0
26	36.22156870213532	38.0	35.0	40.0	31.0	41.0
27	36.13652085127715	38.0	35.0	40.0	31.0	41.0
28	36.0860412368854	38.0	35.0	40.0	30.0	41.0
29	36.04619644346915	38.0	35.0	40.0	30.0	41.0
30	35.94573482920764	38.0	35.0	40.0	30.0	41.0
31	35.7928406171403	38.0	35.0	40.0	30.0	41.0
32	35.50674912733157	38.0	34.0	40.0	28.0	41.0
33	35.19189920418537	38.0	34.0	40.0	26.0	41.0
34	34.8710244592896	38.0	34.0	40.0	24.0	41.0
35	34.548612638425915	37.0	34.0	40.0	23.0	41.0
36	34.40830684508707	37.0	34.0	40.0	22.0	41.0
37	34.34602270842369	37.0	33.0	40.0	22.0	41.0
38	34.23828240668499	37.0	33.0	40.0	21.0	41.0
39	34.1466138253287	37.0	33.0	40.0	21.0	41.0
40	34.07419801126206	37.0	33.0	40.0	21.0	41.0
41	33.958957245697476	37.0	33.0	40.0	20.0	41.0
42	33.90352605964248	37.0	33.0	40.0	20.0	41.0
43	33.84064355687488	37.0	33.0	40.0	19.0	41.0
44	33.692118523178394	36.0	33.0	40.0	18.0	41.0
45	33.6066596795854	36.0	33.0	40.0	19.0	41.0
46	33.568406638300594	36.0	33.0	40.0	19.0	41.0
47	33.51552373006931	36.0	33.0	40.0	19.0	41.0
48	33.362423100126186	36.0	33.0	40.0	18.0	41.0
49	33.30251461205112	36.0	33.0	40.0	18.0	41.0
50	33.229347461490164	35.0	32.0	40.0	18.0	41.0
51	32.83432508112345	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	6.0
13	8.0
14	16.0
15	39.0
16	119.0
17	345.0
18	819.0
19	1535.0
20	2899.0
21	4650.0
22	7396.0
23	11255.0
24	17208.0
25	26790.0
26	37453.0
27	41857.0
28	40870.0
29	40560.0
30	43383.0
31	50651.0
32	60720.0
33	78207.0
34	121921.0
35	175068.0
36	134170.0
37	151555.0
38	214793.0
39	363373.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.926537352420436	22.70363184880382	27.26995157780664	20.099879220969108
2	36.7235216855494	23.778172046842112	27.405843346033766	12.092462921574723
3	27.053335676012402	24.055668935215504	36.27192114837145	12.619074240400646
4	24.055177464082675	25.921907694349187	36.23278775941996	13.790127082148171
5	21.760990216038422	29.817369327040865	35.118008362381325	13.303632094539386
6	19.954035162302198	38.08243936782068	32.085324303370385	9.878201166506734
7	81.65442698766284	3.325170816935604	13.281823063020115	1.7385791323814355
8	82.25236305464053	2.709296053609671	13.055193436894491	1.9831474548553047
9	77.34723541344395	5.229437154971906	14.626119479089747	2.7972079524944
10	38.67638223184414	29.954981244232894	20.822587521793672	10.546049002129298
11	30.976749729383705	23.411350280077112	30.87010049355989	14.741799496979297
12	29.24603413512753	21.66066867104977	32.61291856446197	16.480378629360732
13	21.791830029623423	27.85498652754757	33.560843511905276	16.792339930923735
14	16.921904008315693	31.02442242926809	34.576775777353745	17.476897785062473
15	16.603430714242712	26.70248672106433	41.25420975742213	15.439872807270824
16	19.9386152555097	24.918385075004636	39.7829909212995	15.360008748186162
17	19.436638927216812	25.85304030186157	33.239237089360515	21.471083681561108
18	21.49578010598575	26.426279944414617	35.50289169327778	16.575048256321857
19	23.030767321592908	28.69515642911819	31.637594101363465	16.636482147925438
20	25.12719887256522	27.478642507584013	31.483579335113276	15.910579284737485
21	21.839195560049788	28.301672353397233	33.18873843046236	16.670393656090617
22	21.417083290841557	24.96415332424931	33.88460012065616	19.73416326425297
23	19.287723173969724	28.679429352867675	32.8105513937507	19.222296079411905
24	19.63046285522612	26.322948138737384	36.94935267108417	17.09723633495232
25	18.669145319413232	28.36482639396572	34.518659315896755	18.44736897072429
26	17.989440742711178	31.53671965135038	32.487286256132634	17.986553349805806
27	17.626182141659182	30.861868352085004	33.92735810921226	17.584591397043557
28	17.09035573909272	29.098101324146096	36.94892263384295	16.862620302918234
29	17.54834540099744	27.520540421657657	36.49873507617188	18.43237910117302
30	18.55782710782754	29.625265547996456	34.80635423027634	17.01055311389966
31	22.474544867014053	28.02085803487725	31.96245652016322	17.542140577945478
32	22.578798181065338	28.924980617607197	31.44985212862291	17.046369072704554
33	21.755153996336084	29.57912869540216	30.58529297208567	18.080424336176083
34	18.575581502500974	30.045289064890163	31.308185574585046	20.070943858023817
35	18.926307589665836	30.30767321592907	31.671874212878265	19.094144981526828
36	21.607159751463048	30.082087965960707	29.45699811889424	18.853754163682005
37	19.40819503540435	32.30974230939828	30.180259324743236	18.101803330454132
38	19.347866953849632	32.12415052286385	28.51791965184185	20.010062871444667
39	19.561779764413313	31.413790434251602	29.41497733703739	19.609452464297693
40	21.573186809406266	29.652972233109676	28.945376669619588	19.828464287864474
41	18.07575536041421	29.160702459690153	30.36148930497381	22.402052874921825
42	20.025114174887545	29.458841135642345	28.738283021023907	21.777761668446203
43	20.05552395123132	29.485319142923494	29.381495866113433	21.077661039731755
44	19.127687886342386	31.20921557521167	29.43727783968949	20.225818698756456
45	18.460761559093875	32.81380739000569	27.94216121973306	20.783269831167377
46	20.107558457419554	32.28000830586215	28.14016265237141	19.47227058434689
47	19.593356784697555	30.19973386838157	29.75470675760521	20.452202589315664
48	19.92012365413702	28.759170544169127	31.606201382754033	19.714504418939825
49	19.413232614515845	28.60552438126856	30.85719937632313	21.124043627892462
50	18.492645748836132	30.762591183253612	30.81431851998383	19.93044454792642
51	17.635212923724907	31.846960803948477	29.360239739618592	21.157586532708017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1092.0
1	1715.5
2	2339.0
3	26492.0
4	50645.0
5	34310.5
6	17976.0
7	17616.5
8	17257.0
9	19201.0
10	21145.0
11	22569.5
12	23994.0
13	24046.0
14	24098.0
15	22553.0
16	21008.0
17	19412.5
18	17817.0
19	16424.5
20	15032.0
21	14193.0
22	13354.0
23	13656.5
24	13959.0
25	15113.0
26	18268.5
27	20270.0
28	23240.5
29	26211.0
30	29262.5
31	32314.0
32	37150.5
33	41987.0
34	46840.0
35	51693.0
36	56152.0
37	60611.0
38	66509.0
39	72407.0
40	83289.5
41	94172.0
42	103710.5
43	113249.0
44	116315.5
45	119382.0
46	121939.5
47	124497.0
48	119732.0
49	114967.0
50	109854.5
51	104742.0
52	94812.0
53	84882.0
54	76026.5
55	67171.0
56	62420.0
57	57669.0
58	53555.5
59	49442.0
60	45210.5
61	40979.0
62	36116.5
63	31254.0
64	27155.0
65	23056.0
66	19052.0
67	15048.0
68	12624.5
69	10201.0
70	8388.5
71	6576.0
72	5328.5
73	4081.0
74	3267.0
75	1822.0
76	1191.0
77	970.5
78	750.0
79	512.5
80	275.0
81	221.5
82	168.0
83	107.5
84	47.0
85	34.5
86	22.0
87	15.5
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1627766.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.722061285780953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70432094715088	20.229755039481965
2	7.38551822433138	3.947125412356705
3	3.1636971757376395	2.536215294591399
4	1.8068830014626571	1.9313455320528385
5	1.2209238278525385	1.63128006765729
6	0.8594581748742518	1.3779896412933121
7	0.6796906011002747	1.2713913728579584
8	0.5788344062359204	1.2374118782203913
9	0.5113928714816482	1.2298924487559697
>10	7.171617601155374	45.33646745430215
>50	0.8731901781876096	14.596070872332623
>100	0.04191171912688433	1.7369451030451633
>500	0.0011642142286217433	0.20937167269796664
>1k	0.0011642142286217433	0.5304911980454274
>5k	0.0	0.0
>10k+	2.3284284572434863E-4	2.1982470123088147
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35330	2.170459390354633	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT	2334	0.14338670300276576	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCT	1751	0.1075707441978761	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1649	0.10130448725431052	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0340343759483857	0.0
2	0.0	0.0	0.0	0.09970720607261732	0.0
3	0.0	0.0	0.0	0.21256126494840166	0.0
4	0.0	0.0	0.0	0.34243251179837886	0.0
5	0.0	0.0	0.0	0.6325847818421075	0.0
6	0.0	0.0	0.0	1.232671035025919	0.0
7	0.0	0.0	0.0	1.6315612932079917	0.0
8	0.0	0.0	0.0	2.2578798181065336	0.0
9	0.0	0.0	0.0	2.618742497385988	0.0
10	0.0	0.0	0.0	3.0330526623605603	0.0
11	0.0	0.0	0.0	3.3364132190990596	0.0
12	0.0	0.0	0.0	3.5727494000980484	0.0
13	0.0	0.0	0.0	3.7120814662549777	0.0
14	0.0	0.0	0.0	3.789303868000683	0.0
15	0.0	0.0	0.0	3.8569425826562296	0.0
16	0.0	0.0	0.0	3.964881930203727	0.0
17	0.0	0.0	0.0	4.089654164050607	0.0
18	0.0	0.0	0.0	4.244037533650414	0.0
19	0.0	0.0	0.0	4.341041648492474	0.0
20	0.0	0.0	0.0	4.440441685107073	0.0
21	0.0	0.0	0.0	4.565091051170746	0.0
22	0.0	0.0	0.0	4.711488014862087	0.0
23	0.0	0.0	0.0	4.869495984066506	0.0
24	0.0	0.0	0.0	4.995251160179043	0.0
25	0.0	0.0	0.0	5.107183710680774	0.0
26	0.0	0.0	0.0	5.210146913008381	0.0
27	0.0	0.0	0.0	5.324045348041426	0.0
28	0.0	0.0	0.0	5.439049593123336	0.0
29	0.0	0.0	0.0	5.569596612780953	0.0
30	0.0	0.0	0.0	5.7469562578405	0.0
31	0.0	0.0	0.0	5.895810577195985	0.0
32	0.0	0.0	0.0	6.04620074384156	0.0
33	0.0	0.0	0.0	6.189526012952721	0.0
34	0.0	0.0	0.0	6.342434969154043	0.0
35	0.0	0.0	0.0	6.513589791161629	0.0
36	0.0	0.0	0.0	6.6662530117965355	0.0
37	0.0	0.0	0.0	6.828499919521602	0.0
38	0.0	0.0	0.0	7.028344368908062	0.0
39	0.0	0.0	0.0	7.282373510688883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16430	0.0	43.46622	1
CCGAACC	55	6.184564E-11	40.909092	18
GCGATAC	50	1.0822987E-9	40.5	9
TAACGCG	45	1.9292202E-8	40.000004	1
TACGCGG	80	0.0	39.375	2
TCACGAC	110	0.0	38.863636	25
AATCGGT	35	6.249893E-6	38.571426	26
GGGCGAT	2200	0.0	38.147728	7
TAGGGAC	1540	0.0	37.694805	5
GTTTTTT	19435	0.0	37.636997	2
CGGGACC	365	0.0	37.602737	6
AAGGGCG	485	0.0	37.57732	5
TTACGCG	30	1.1401305E-4	37.500004	1
TAATGCG	60	1.5643309E-10	37.500004	1
AGGGATC	1585	0.0	37.47634	6
GGCACCG	495	0.0	37.272728	8
GACCGAT	750	0.0	37.2	9
TATGGGC	660	0.0	37.159092	4
ATATGCG	85	0.0	37.058823	1
CTCGTAG	55	2.750312E-9	36.81818	1
>>END_MODULE