FastQCFastQC Report
Thu 26 May 2016
SRR1547640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547640_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1827560
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124700.6823305390794283No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44930.2458469215784981No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT25670.1404605047166714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCA302.1662072E-645.00000430
TCCGCGT207.0343807E-445.041
GTCGAAC207.0343807E-445.036
TAGGTCG207.0343807E-445.01
CGTTCGA207.0343807E-445.014
GTGTCGA502.1827873E-1145.036
CGTAAGA207.0343807E-445.041
ACGTATC253.8917133E-545.027
CCGCGTA207.0343807E-445.034
CGCCGAC207.0343807E-445.013
ATACCCG207.0343807E-445.018
TCGCAAG900.042.51
CGTTTTT56450.042.449071
ATCGTAG1500.042.0000041
CGACGGT2500.041.39999828
CGACAGG1850.041.3513532
TAGGGCG3450.041.0869565
CGGGATA5300.040.754726
CGCCGGT501.0822987E-940.528
GACCGAT9800.040.4081659