FastQCFastQC Report
Thu 26 May 2016
SRR1547639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547639_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2772696
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455091.6413267087340262No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT32600.11757509658469589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA207.035013E-445.018
CTACGCG453.8562575E-1045.01
TCGTACT207.035013E-445.034
CGTTTTT223850.043.7435761
GCGATCG1000.042.759
TAACGCG1400.041.7857131
CGTTAGG3450.039.7826082
CAGCGCG1250.039.61
GTGCGTA403.4605728E-739.37523
TAGGGAC35550.039.1772165
TACGGGA15050.039.1694374
TCGTCCC4550.039.06593338
TGGGCGA11100.038.918926
CGGGATC8600.038.7209326
ACGGGAT16850.038.4569745
GGGCGAT38650.038.4217387
TAGCGGA1000.038.253
TAGGGAT56650.038.128865
TCGCGAG659.094947E-1238.076921
AGGGCGA17800.038.0477526
Produced by FastQC (version 0.11.5)