##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547639_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2772696
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292171951054137	31.0	31.0	33.0	30.0	34.0
2	31.690339294318598	31.0	31.0	34.0	30.0	34.0
3	31.647867274306307	31.0	31.0	34.0	30.0	34.0
4	35.52211962652956	37.0	35.0	37.0	33.0	37.0
5	35.47093839353467	37.0	35.0	37.0	33.0	37.0
6	35.53121510616382	37.0	35.0	37.0	33.0	37.0
7	35.933575119666926	37.0	35.0	37.0	35.0	37.0
8	35.977587878368205	37.0	35.0	37.0	35.0	37.0
9	37.790530588279424	39.0	38.0	39.0	35.0	39.0
10	37.177295311134	39.0	37.0	39.0	33.0	39.0
11	36.848721605253516	39.0	37.0	39.0	32.0	39.0
12	36.53713317291185	39.0	35.0	39.0	32.0	39.0
13	36.43817930274361	39.0	35.0	39.0	32.0	39.0
14	37.52491329738277	40.0	36.0	41.0	32.0	41.0
15	37.690549919644994	40.0	36.0	41.0	33.0	41.0
16	37.72211089856226	40.0	36.0	41.0	33.0	41.0
17	37.604495047419555	40.0	36.0	41.0	32.0	41.0
18	37.44104979413538	39.0	36.0	41.0	32.0	41.0
19	37.319967641602254	39.0	36.0	41.0	32.0	41.0
20	37.10013430971156	39.0	35.0	41.0	32.0	41.0
21	36.948586502090386	39.0	35.0	41.0	32.0	41.0
22	36.90135305132622	38.0	35.0	41.0	31.0	41.0
23	36.80511386751378	38.0	35.0	41.0	31.0	41.0
24	36.73044430402756	38.0	35.0	40.0	31.0	41.0
25	36.639831773840335	38.0	35.0	40.0	31.0	41.0
26	36.5554377400191	38.0	35.0	40.0	31.0	41.0
27	36.47744577840484	38.0	35.0	40.0	31.0	41.0
28	36.4385670120345	38.0	35.0	40.0	31.0	41.0
29	36.43501234899174	38.0	35.0	40.0	31.0	41.0
30	36.381118954259684	38.0	35.0	40.0	30.0	41.0
31	36.26011614688375	38.0	35.0	40.0	30.0	41.0
32	36.03568187785462	38.0	35.0	41.0	30.0	41.0
33	35.770937744347016	38.0	35.0	41.0	28.0	41.0
34	35.51452485234588	38.0	35.0	41.0	26.0	41.0
35	35.239677195047705	38.0	34.0	41.0	25.0	41.0
36	35.139400064053184	38.0	34.0	40.0	24.0	41.0
37	35.10102225415264	38.0	34.0	40.0	24.0	41.0
38	35.002603242475914	38.0	34.0	40.0	24.0	41.0
39	34.91386938921541	38.0	34.0	40.0	23.0	41.0
40	34.8556246339303	38.0	34.0	40.0	23.0	41.0
41	34.75090669875096	38.0	34.0	40.0	23.0	41.0
42	34.71003348365634	38.0	34.0	40.0	23.0	41.0
43	34.65905097421427	38.0	34.0	40.0	23.0	41.0
44	34.53227183939386	38.0	34.0	40.0	23.0	41.0
45	34.45745368406778	38.0	34.0	40.0	23.0	41.0
46	34.43347810217925	38.0	34.0	40.0	23.0	41.0
47	34.39276970861573	38.0	33.0	40.0	23.0	41.0
48	34.25532117476997	37.0	33.0	40.0	22.0	41.0
49	34.18316757408674	37.0	33.0	40.0	22.0	41.0
50	34.10263043622525	37.0	33.0	40.0	21.0	41.0
51	33.715358625684175	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	7.0
14	15.0
15	48.0
16	168.0
17	488.0
18	1082.0
19	2265.0
20	4164.0
21	6553.0
22	10473.0
23	15651.0
24	25096.0
25	38864.0
26	54222.0
27	60212.0
28	60680.0
29	62789.0
30	69607.0
31	81739.0
32	99401.0
33	124919.0
34	188595.0
35	270332.0
36	209381.0
37	260997.0
38	394021.0
39	730738.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.743794487387003	23.67075222094308	28.275728749202944	18.30972454246697
2	34.70946688710194	24.700363833611764	28.37400854619475	12.216160733091547
3	27.00032747910337	24.50596819846099	35.955077657269314	12.538626665166323
4	23.467845014383112	27.548891043230128	35.520843251477984	13.462420690908777
5	21.72791391483235	31.022441695735846	34.06886294061808	13.180781448813716
6	20.708400776716957	38.84883160649418	30.471245315029126	9.971522301759732
7	84.63459391148542	2.682804029002819	11.20674606952944	1.4758559899823132
8	85.2919324729433	1.992537227305121	10.797180794432567	1.9183495053190107
9	80.13933009605093	5.095293533802479	12.419140071612611	2.34623629853399
10	36.72634865127659	38.22146387487124	16.572281995573984	8.47990547827818
11	25.13380478783105	24.055107375637284	34.91356427101998	15.89752356551169
12	26.354097239654113	22.753161543854787	33.725190211981406	17.16755100450969
13	20.84061866140392	27.39965001572477	35.05487078280489	16.70486054006642
14	15.866326492338143	30.936604662032906	34.933472692282166	18.26359615334678
15	15.171226849247086	28.038270333278515	41.2528456058652	15.537657211609208
16	19.192150888521496	26.39820593386365	39.05191914295689	15.357724034657966
17	18.96017450164028	26.190213424046487	33.85362116871089	20.995990905602348
18	19.896483422632702	26.91070351744295	35.761295143787855	17.431517916136496
19	21.87434179585501	28.582614177681215	32.6725324377429	16.87051158872087
20	23.24870811657679	27.835218862796353	32.653201072169466	16.262871948457384
21	21.722504017750232	27.50041836537435	34.36211542844943	16.414962188425992
22	20.648062391261067	24.61160545548448	34.71675221517253	20.023579938081927
23	17.55367339225072	28.673392250719154	34.42021772311137	19.352716633918757
24	17.74990839240941	27.351826525518845	37.52769867306044	17.3705664090113
25	17.972687954251025	28.96177583117659	34.796061306396375	18.269474908176015
26	17.401042162573898	31.991065735298786	32.56238693315098	18.04550516897633
27	17.12665218256888	30.89083693271819	34.47637245482375	17.506138429889177
28	16.225074800843657	29.44570915816231	37.14821242574015	17.18100361525389
29	16.823265154203707	27.40661074997043	36.80165441865967	18.968469677166194
30	17.804151627152777	29.057350679627337	34.98295521759328	18.15554247562661
31	20.63446551659468	28.051867207944902	32.93354193896482	18.3801253364956
32	20.8968455250774	28.796846102133088	32.05086313104646	18.255445241743054
33	20.03173084968565	28.819351273994698	32.00862986782539	19.14028800849426
34	17.55100450969021	29.55480874931835	31.6411535920274	21.25303314896404
35	17.247473217402845	29.70816129860612	32.46219563919016	20.582169844800873
36	20.19345070646043	29.094895365377237	31.12984618580616	19.581807742356176
37	17.487023460199026	31.222102964046545	32.606748089224354	18.68412548653008
38	17.783666150201825	30.627591340702338	30.773261836133496	20.815480672962344
39	17.855401385510707	30.12941195140037	31.489640407747547	20.52554625534137
40	19.903408090897813	29.101134780011943	31.206991318197165	19.78846581089308
41	17.22302048259167	28.714002544815585	32.368099495941856	21.694877476650884
42	19.253463055452166	28.724137085349422	30.79248500376529	21.229914855433123
43	19.3782874141269	28.916368761667343	30.852643059318442	20.852700764887317
44	18.70111256336793	30.86750224330399	30.136553015548767	20.294832177779316
45	17.646831820004792	32.105935883342426	29.652114764835382	20.595117531817408
46	19.53373179028642	31.2071716480999	29.49032277609951	19.76877378551417
47	18.29205221199872	30.262567551581565	30.979523178884378	20.465857057535338
48	19.256853257623625	28.433120688312023	32.11495237847928	20.195073675585064
49	19.017447278749636	27.810730062004634	31.983672209286556	21.188150449959174
50	17.252883114484963	30.6174568001685	31.74296785511286	20.38669223023368
51	16.980188235565674	30.654352298268545	30.748628771419583	21.6168306947462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1557.0
1	2173.0
2	2789.0
3	34993.0
4	67197.0
5	45967.5
6	24738.0
7	23939.0
8	23140.0
9	24302.0
10	25464.0
11	26517.0
12	27570.0
13	27605.5
14	27641.0
15	26977.0
16	26313.0
17	25140.5
18	23968.0
19	23228.5
20	22489.0
21	22324.0
22	22159.0
23	23899.5
24	25640.0
25	28682.0
26	37563.0
27	43402.0
28	49857.5
29	56313.0
30	65235.0
31	74157.0
32	85882.0
33	97607.0
34	104484.0
35	111361.0
36	119905.0
37	128449.0
38	140926.5
39	153404.0
40	170347.5
41	187291.0
42	201485.5
43	215680.0
44	221151.0
45	226622.0
46	232216.5
47	237811.0
48	233078.0
49	228345.0
50	208810.5
51	189276.0
52	166117.5
53	142959.0
54	121768.0
55	100577.0
56	87503.0
57	74429.0
58	65102.0
59	55775.0
60	47366.5
61	38958.0
62	32120.5
63	25283.0
64	20185.5
65	15088.0
66	11586.5
67	8085.0
68	6186.5
69	4288.0
70	3215.0
71	2142.0
72	1873.5
73	1605.0
74	1156.5
75	532.0
76	356.0
77	234.0
78	112.0
79	99.5
80	87.0
81	76.0
82	65.0
83	47.5
84	30.0
85	21.0
86	12.0
87	13.5
88	15.0
89	12.5
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2772696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.570269991689592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40810650548873	18.009596998881435
2	7.974596245862117	3.759267731793687
3	3.2158426902505775	2.2739484138002255
4	1.8148184493063146	1.7110304334419708
5	1.140033020957946	1.3435443051710152
6	0.8255562308679851	1.1675149952928021
7	0.6031326944618903	0.9951200314497387
8	0.5191946821039218	0.9790047068351109
9	0.40096460939042833	0.8505759690400275
>10	5.127705873662696	28.424102858988242
>50	1.5824722679190661	26.332953901841506
>100	0.3839899323063184	11.462264408213981
>500	0.0017154248540207535	0.28527338384946993
>1k	0.0017154248540207535	0.7330140214825577
>5k	0.0	0.0
>10k+	1.559477140018867E-4	1.6727878399182488
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45509	1.6413267087340262	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT	3260	0.11757509658469589	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.213196109490546E-5	0.0	0.0	0.05727277710935494	0.0
2	7.213196109490546E-5	0.0	0.0	0.17308064064722567	0.0
3	7.213196109490546E-5	0.0	0.0	0.3280922250401775	0.0
4	7.213196109490546E-5	0.0	0.0	0.5498258734459169	0.0
5	7.213196109490546E-5	0.0	0.0	0.9890373845527962	0.0
6	7.213196109490546E-5	0.0	0.0	1.764852692109052	0.0
7	7.213196109490546E-5	0.0	0.0	2.227038232824659	0.0
8	7.213196109490546E-5	0.0	0.0	3.1132875728172147	0.0
9	7.213196109490546E-5	0.0	0.0	3.5609745893527456	0.0
10	7.213196109490546E-5	0.0	0.0	4.085410012493256	0.0
11	7.213196109490546E-5	0.0	0.0	4.561372757777989	0.0
12	7.213196109490546E-5	0.0	0.0	4.958711665469276	0.0
13	7.213196109490546E-5	0.0	0.0	5.1659828556754865	0.0
14	7.213196109490546E-5	0.0	0.0	5.296325309373981	0.0
15	7.213196109490546E-5	0.0	0.0	5.417290608130138	0.0
16	7.213196109490546E-5	0.0	0.0	5.60230908833857	0.0
17	7.213196109490546E-5	0.0	0.0	5.836665829935918	0.0
18	7.213196109490546E-5	0.0	0.0	6.101606523037506	0.0
19	7.213196109490546E-5	0.0	0.0	6.287057795012507	0.0
20	7.213196109490546E-5	0.0	0.0	6.4766566547504665	0.0
21	7.213196109490546E-5	0.0	0.0	6.702177231113689	0.0
22	7.213196109490546E-5	0.0	0.0	6.965026097343524	0.0
23	7.213196109490546E-5	0.0	0.0	7.229750394561827	0.0
24	7.213196109490546E-5	0.0	0.0	7.433378920732745	0.0
25	7.213196109490546E-5	0.0	0.0	7.617531817408039	0.0
26	7.213196109490546E-5	0.0	0.0	7.797645324262018	0.0
27	7.213196109490546E-5	0.0	0.0	7.980391647695961	0.0
28	7.213196109490546E-5	0.0	0.0	8.171757740480745	0.0
29	7.213196109490546E-5	0.0	0.0	8.385160147380024	0.0
30	7.213196109490546E-5	0.0	0.0	8.666691191533438	0.0
31	7.213196109490546E-5	0.0	0.0	8.909703768462176	0.0
32	7.213196109490546E-5	0.0	0.0	9.13320464991474	0.0
33	7.213196109490546E-5	0.0	0.0	9.344911955728287	0.0
34	7.213196109490546E-5	0.0	0.0	9.57082925787753	0.0
35	7.213196109490546E-5	0.0	0.0	9.823543583573533	0.0
36	7.213196109490546E-5	0.0	0.0	10.069621768848803	0.0
37	7.213196109490546E-5	0.0	0.0	10.322155764642067	0.0
38	7.213196109490546E-5	0.0	0.0	10.599575287013073	0.0
39	7.213196109490546E-5	0.0	0.0	10.958792453265703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.035013E-4	45.0	18
CTACGCG	45	3.8562575E-10	45.0	1
TCGTACT	20	7.035013E-4	45.0	34
CGTTTTT	22385	0.0	43.743576	1
GCGATCG	100	0.0	42.75	9
TAACGCG	140	0.0	41.785713	1
CGTTAGG	345	0.0	39.782608	2
CAGCGCG	125	0.0	39.6	1
GTGCGTA	40	3.4605728E-7	39.375	23
TAGGGAC	3555	0.0	39.177216	5
TACGGGA	1505	0.0	39.169437	4
TCGTCCC	455	0.0	39.065933	38
TGGGCGA	1110	0.0	38.91892	6
CGGGATC	860	0.0	38.720932	6
ACGGGAT	1685	0.0	38.456974	5
GGGCGAT	3865	0.0	38.421738	7
TAGCGGA	100	0.0	38.25	3
TAGGGAT	5665	0.0	38.12886	5
TCGCGAG	65	9.094947E-12	38.07692	1
AGGGCGA	1780	0.0	38.047752	6
>>END_MODULE