FastQCFastQC Report
Thu 26 May 2016
SRR1547638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547638_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1777324
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89560.5039036213993622No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38890.21881210178898164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG406.8193913E-945.032
CGTATAT207.0343283E-445.027
CGAGTGT502.1827873E-1145.028
CGACGGT1050.045.028
GACACGT207.0343283E-445.012
CGTTGCA207.0343283E-445.011
ATAGCCG207.0343283E-445.01
CCTCGAA207.0343283E-445.013
AAACGTC253.8916693E-545.036
CGTACTC207.0343283E-445.015
TACTCGC1000.045.045
ACCGTTA253.8916693E-545.040
CCGCGTA253.8916693E-545.027
CCAACGA207.0343283E-445.033
CGATCCA207.0343283E-445.024
GCGTATT253.8916693E-545.029
GTAACGT253.8916693E-545.031
TCGAAAC207.0343283E-445.023
GTACGGA351.2124292E-745.018
TGCGTAA253.8916693E-545.022