##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547638_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1777324
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291373998213043	31.0	31.0	33.0	30.0	34.0
2	31.69358710060743	31.0	31.0	34.0	30.0	34.0
3	31.83584028573293	31.0	31.0	34.0	30.0	34.0
4	35.592458099930006	37.0	35.0	37.0	33.0	37.0
5	35.477242753712886	37.0	35.0	37.0	33.0	37.0
6	35.52503764085783	37.0	35.0	37.0	33.0	37.0
7	35.873001208558485	37.0	35.0	37.0	35.0	37.0
8	35.90644136915948	37.0	35.0	37.0	35.0	37.0
9	37.728508701846145	39.0	37.0	39.0	35.0	39.0
10	37.140452162914585	39.0	37.0	39.0	33.0	39.0
11	36.78710803432576	39.0	35.0	39.0	32.0	39.0
12	35.80504342483419	37.0	35.0	39.0	32.0	39.0
13	35.42635613990471	37.0	35.0	39.0	30.0	39.0
14	36.34833941363533	38.0	35.0	41.0	31.0	41.0
15	36.67436213093392	38.0	35.0	41.0	32.0	41.0
16	36.83839637567489	38.0	35.0	41.0	32.0	41.0
17	36.75908388116066	38.0	35.0	41.0	32.0	41.0
18	36.704558651095695	38.0	35.0	40.0	32.0	41.0
19	36.64324512581837	38.0	35.0	40.0	31.0	41.0
20	36.46095759692662	37.0	35.0	40.0	31.0	41.0
21	36.2437349633494	37.0	35.0	40.0	31.0	41.0
22	36.13687656274264	37.0	35.0	40.0	31.0	41.0
23	36.147524593152404	37.0	35.0	40.0	31.0	41.0
24	36.06255865559684	37.0	35.0	40.0	31.0	41.0
25	35.95467117981865	37.0	35.0	40.0	30.0	41.0
26	35.810618097769456	36.0	34.0	40.0	30.0	41.0
27	35.73394665238302	36.0	34.0	40.0	30.0	41.0
28	35.77592492983834	36.0	35.0	40.0	30.0	41.0
29	35.86885565040477	36.0	35.0	40.0	30.0	41.0
30	35.91066569741927	36.0	35.0	40.0	30.0	41.0
31	35.78263445494463	36.0	35.0	40.0	30.0	41.0
32	35.62219268968404	36.0	34.0	40.0	30.0	41.0
33	35.51454940123467	36.0	34.0	40.0	29.0	41.0
34	35.45178650600566	36.0	34.0	40.0	29.0	41.0
35	35.29394302895814	36.0	34.0	40.0	29.0	41.0
36	35.144913364136194	36.0	34.0	40.0	27.0	41.0
37	35.084665485865266	36.0	34.0	40.0	27.0	41.0
38	35.03819281121506	36.0	34.0	40.0	27.0	41.0
39	35.01920358921615	36.0	34.0	40.0	27.0	41.0
40	34.919301714262566	36.0	34.0	40.0	27.0	41.0
41	34.880862465144226	36.0	34.0	40.0	27.0	41.0
42	34.825528153561194	36.0	34.0	40.0	27.0	41.0
43	34.770921902815694	36.0	34.0	40.0	27.0	41.0
44	34.62483936524798	35.0	34.0	40.0	26.0	41.0
45	34.5495897202761	35.0	34.0	40.0	26.0	41.0
46	34.52875165135901	35.0	34.0	40.0	26.0	41.0
47	34.483751415048694	35.0	33.0	40.0	26.0	41.0
48	34.391504869117846	35.0	34.0	40.0	26.0	41.0
49	34.36052683697514	36.0	34.0	40.0	25.0	41.0
50	34.24816240595412	35.0	34.0	40.0	24.0	41.0
51	33.80671616430094	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	4.0
13	9.0
14	15.0
15	39.0
16	127.0
17	319.0
18	720.0
19	1472.0
20	2624.0
21	4280.0
22	6435.0
23	9514.0
24	13984.0
25	19786.0
26	26355.0
27	31640.0
28	35479.0
29	40344.0
30	48207.0
31	58555.0
32	73021.0
33	95355.0
34	173458.0
35	288744.0
36	115429.0
37	141914.0
38	210107.0
39	379233.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.55506593057878	24.47229655369533	29.274459805865447	13.698177709860444
2	29.631007064553227	27.02984936905145	30.18352309426981	13.15562047212551
3	29.255836302216142	25.86568346570462	31.496395705003703	13.382084527075536
4	26.598020394705753	27.361246458158444	31.141367584075834	14.899365563059972
5	23.696129687102633	32.75559211488733	29.571535634470695	13.976742563539343
6	21.804578118564763	41.804251785268185	26.132995447087872	10.258174649079177
7	88.13092041743656	4.18961314875622	6.2911433143309825	1.3883231194762462
8	88.84772838266967	3.2302495211902835	5.603367759620643	2.318654336519397
9	84.03307444225139	5.681969072605782	7.6504340232844426	2.6345224618583893
10	50.564669131795895	27.863012033821633	12.202670981768096	9.36964785261438
11	45.41321672356869	18.78667029759346	22.198935028165938	13.601177950671909
12	42.583288134296275	20.049748948419083	23.22896669374858	14.137996223536057
13	21.760523123527282	40.28421379557132	23.058260621023514	14.897002459877884
14	15.828965343403903	40.936374009465915	26.806986233236035	16.42767441389415
15	14.551426751678365	25.14195498400967	45.90524856469614	14.401369699615826
16	16.709615129261742	21.07331021243172	44.762969497964356	17.454105160342177
17	17.426816945025216	21.504745336247076	27.09534108581215	33.97309663291555
18	21.70048904982997	25.13632854786184	33.78387958526414	19.37930281704405
19	28.52687523490371	26.21930497759553	25.706511587082602	19.547308200418158
20	31.12330672404131	23.486938791126434	26.30538945065728	19.084365034174976
21	22.298466683620994	29.748712108765762	28.255455955132547	19.697365252480694
22	22.118251933806103	23.910046789443005	27.4108153606208	26.560885916130093
23	19.518163261172415	30.184085737884597	25.929262194175063	24.368488806767928
24	19.92152246861011	23.887034665598396	38.50654129466547	17.68490157112603
25	17.49174601817114	26.031213217173683	34.83489785767817	21.642142906977003
26	16.798231498590017	36.181529085299026	26.68748072945619	20.33275868665477
27	17.245983287234065	35.64060351404696	29.04478868231116	18.068624516407812
28	14.290528907503642	29.397847550587286	38.87270975916603	17.438913782743047
29	15.462234235288557	24.341706970704273	38.51177388028294	21.684284913724227
30	17.328748162968598	31.85946962962296	32.42948387575929	18.382298331649153
31	29.031847879171156	26.63273550573784	25.92678656227002	18.408630052820982
32	27.874152377394328	27.39264197186332	27.033168966378668	17.700036684363685
33	26.401376451339203	29.232542856564137	25.008946033475045	19.357134658621614
34	18.337736957358366	27.937618577141816	28.363033414278995	25.361611051220823
35	19.59822744755599	26.66756314549289	31.76955917998069	21.964650226970434
36	28.660165507245722	25.724291125309733	27.270435778732523	18.34510758871202
37	19.003738204176614	32.565587366175215	30.494890070690545	17.935784358957623
38	19.617582387904513	32.0551008144829	25.863095305076623	22.46422149253597
39	19.66928933610304	31.76432659436321	28.88274732125375	19.683636748279998
40	23.654887910139063	27.099673441645983	26.649220963650972	22.596217684563985
41	16.703932428752438	24.631637225401786	29.466546335952255	29.197884009893528
42	21.54199234354569	25.41281162016605	25.910469897441324	27.134726138846943
43	22.902127017921327	25.44398207642501	26.714768944773155	24.939121960880513
44	18.60848106479179	30.30010285125278	29.09666442359412	21.99475166036131
45	17.197595936362756	37.30585982071924	23.961191094026752	21.535353148891254
46	21.492198383637422	33.49484956035028	25.615419585849285	19.39753247016301
47	20.65549106409411	27.444067598254456	28.219109177617586	23.68133216003385
48	22.508220223211975	23.628556188967234	32.63991258768801	21.22331100013278
49	20.37878293434399	24.052789474513368	33.37821353900583	22.19021405213681
50	18.17614571119278	31.46983892638596	29.794680092093508	20.55933527032775
51	17.728000072018382	33.00613731655005	25.881099900749664	23.3847627106819
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	853.0
1	1619.5
2	2386.0
3	8393.0
4	14400.0
5	10326.0
6	6252.0
7	6196.0
8	6140.0
9	6637.5
10	7135.0
11	7230.0
12	7325.0
13	7285.5
14	7246.0
15	6900.5
16	6555.0
17	6175.0
18	5795.0
19	5965.0
20	6135.0
21	6147.5
22	6160.0
23	6218.5
24	6277.0
25	8302.0
26	12947.5
27	15568.0
28	19849.0
29	24130.0
30	28157.5
31	32185.0
32	36556.0
33	40927.0
34	46966.5
35	53006.0
36	54381.0
37	55756.0
38	65187.0
39	74618.0
40	104241.5
41	133865.0
42	161276.0
43	188687.0
44	187518.5
45	186350.0
46	184678.5
47	183007.0
48	171523.5
49	160040.0
50	145681.5
51	131323.0
52	116296.5
53	101270.0
54	89503.0
55	77736.0
56	67903.5
57	58071.0
58	52766.5
59	47462.0
60	41287.0
61	35112.0
62	31714.5
63	28317.0
64	23961.5
65	19606.0
66	16684.5
67	13763.0
68	11593.0
69	9423.0
70	7896.0
71	6369.0
72	5105.5
73	3842.0
74	2774.5
75	1409.5
76	1112.0
77	885.5
78	659.0
79	451.5
80	244.0
81	171.0
82	98.0
83	60.0
84	22.0
85	24.5
86	27.0
87	28.5
88	30.0
89	17.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1777324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.94356224916656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22952021865771	13.424303077964275
2	8.255964812882736	2.797709074680148
3	2.775199262923084	1.4106528439553532
4	1.2264667478724534	0.8312286275643912
5	0.7147447751285347	0.6055161294828443
6	0.4902412392117793	0.4983859772216002
7	0.336957656353461	0.39964841180306976
8	0.2644721155071051	0.35848798018107236
9	0.21258029836393202	0.32416807664481134
>10	3.1971271095249185	13.965814714109861
>50	1.6465106481675276	20.12039290281867
>100	1.6384278645824375	43.03889052435731
>500	0.008082686279543226	0.8308269552487457
>1k	0.0033677859498096774	0.8829248268244396
>5k	3.3677859498096774E-4	0.5110498771433906
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8956	0.5039036213993622	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3889	0.21881210178898164	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.626436147826733E-5	0.0	0.0	0.04129804132504822	0.0
2	5.626436147826733E-5	0.0	0.0	0.15770900522358333	0.0
3	5.626436147826733E-5	0.0	0.0	0.2469442825281153	0.0
4	5.626436147826733E-5	0.0	0.0	0.543007352626758	0.0
5	5.626436147826733E-5	0.0	0.0	0.8928028879371459	0.0
6	5.626436147826733E-5	0.0	0.0	1.3452808829453717	0.0
7	5.626436147826733E-5	0.0	0.0	1.5459758603383513	0.0
8	5.626436147826733E-5	0.0	0.0	2.1374830925593757	0.0
9	5.626436147826733E-5	0.0	0.0	2.3099896248517435	0.0
10	5.626436147826733E-5	0.0	0.0	2.638292174077433	0.0
11	5.626436147826733E-5	0.0	0.0	3.038331784187914	0.0
12	5.626436147826733E-5	0.0	0.0	3.3765368610337787	0.0
13	5.626436147826733E-5	0.0	0.0	3.509770869014316	0.0
14	5.626436147826733E-5	0.0	0.0	3.558833392223365	0.0
15	5.626436147826733E-5	0.0	0.0	3.6401353945594614	0.0
16	5.626436147826733E-5	0.0	0.0	3.8234446842556564	0.0
17	5.626436147826733E-5	0.0	0.0	4.0308913850260275	0.0
18	5.626436147826733E-5	0.0	0.0	4.310243939765625	0.0
19	5.626436147826733E-5	0.0	0.0	4.452198923775294	0.0
20	5.626436147826733E-5	0.0	0.0	4.606419538587224	0.0
21	5.626436147826733E-5	0.0	0.0	4.805201527689943	0.0
22	5.626436147826733E-5	0.0	0.0	5.010341389639706	0.0
23	5.626436147826733E-5	0.0	0.0	5.2324730887559046	0.0
24	5.626436147826733E-5	0.0	0.0	5.404191919987577	0.0
25	5.626436147826733E-5	0.0	0.0	5.547497248672724	0.0
26	5.626436147826733E-5	0.0	0.0	5.684557233233783	0.0
27	5.626436147826733E-5	0.0	0.0	5.815540666755189	0.0
28	5.626436147826733E-5	0.0	0.0	5.953557145461379	0.0
29	5.626436147826733E-5	0.0	0.0	6.106483679959309	0.0
30	5.626436147826733E-5	0.0	0.0	6.304140381832463	0.0
31	5.626436147826733E-5	0.0	0.0	6.484974039623614	0.0
32	5.626436147826733E-5	0.0	0.0	6.6565803421323295	0.0
33	5.626436147826733E-5	0.0	0.0	6.825598484013044	0.0
34	5.626436147826733E-5	0.0	0.0	6.997035993437325	0.0
35	5.626436147826733E-5	0.0	0.0	7.220799359036394	0.0
36	5.626436147826733E-5	0.0	0.0	7.413279739653547	0.0
37	5.626436147826733E-5	0.0	0.0	7.600077419761394	0.0
38	5.626436147826733E-5	0.0	0.0	7.784962111578981	0.0
39	5.626436147826733E-5	0.0	0.0	7.969284159781784	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	40	6.8193913E-9	45.0	32
CGTATAT	20	7.0343283E-4	45.0	27
CGAGTGT	50	2.1827873E-11	45.0	28
CGACGGT	105	0.0	45.0	28
GACACGT	20	7.0343283E-4	45.0	12
CGTTGCA	20	7.0343283E-4	45.0	11
ATAGCCG	20	7.0343283E-4	45.0	1
CCTCGAA	20	7.0343283E-4	45.0	13
AAACGTC	25	3.8916693E-5	45.0	36
CGTACTC	20	7.0343283E-4	45.0	15
TACTCGC	100	0.0	45.0	45
ACCGTTA	25	3.8916693E-5	45.0	40
CCGCGTA	25	3.8916693E-5	45.0	27
CCAACGA	20	7.0343283E-4	45.0	33
CGATCCA	20	7.0343283E-4	45.0	24
GCGTATT	25	3.8916693E-5	45.0	29
GTAACGT	25	3.8916693E-5	45.0	31
TCGAAAC	20	7.0343283E-4	45.0	23
GTACGGA	35	1.2124292E-7	45.0	18
TGCGTAA	25	3.8916693E-5	45.0	22
>>END_MODULE