FastQCFastQC Report
Thu 26 May 2016
SRR1547636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547636_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2389964
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240761.0073791906488967No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31030.1298345916507529No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA207.034816E-445.00000442
ACACGTC253.892074E-545.015
CTATACG502.1827873E-1145.01
GTTAGCG1250.044.9999961
CATACCG302.1664891E-644.99999621
CGTTTTT121050.043.2899631
TTTAGCG1000.042.751
CGTAAGG4950.041.818182
TCGCGAG1200.041.2499961
GCGTAAG2550.040.5882341
TGTAGCG1900.040.263161
TATAGCG1850.040.1351361
CGGGTAC2250.040.06
TAGTACG900.040.01
CATACGA8150.039.754618
CTTCGCG403.460318E-739.3750041
CGATCTA800.039.37500410
ACGTAGG3900.039.230772
CGAGGGA13550.039.0221374
AGGGATC24500.038.846946
Produced by FastQC (version 0.11.5)