##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547636_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2389964
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261785114754865	31.0	31.0	33.0	30.0	34.0
2	31.66195432232452	31.0	31.0	34.0	30.0	34.0
3	31.728464529172825	31.0	31.0	34.0	30.0	34.0
4	35.53966084844792	37.0	35.0	37.0	33.0	37.0
5	35.45568761705197	37.0	35.0	37.0	33.0	37.0
6	35.51630610335553	37.0	35.0	37.0	33.0	37.0
7	35.90195417169463	37.0	35.0	37.0	35.0	37.0
8	35.94126647932772	37.0	35.0	37.0	35.0	37.0
9	37.76622995158086	39.0	38.0	39.0	35.0	39.0
10	37.06800646369569	39.0	37.0	39.0	33.0	39.0
11	36.74736481386331	39.0	35.0	39.0	32.0	39.0
12	36.21804930952935	38.0	35.0	39.0	32.0	39.0
13	36.03368921038141	38.0	35.0	39.0	32.0	39.0
14	37.078081092434864	39.0	35.0	41.0	32.0	41.0
15	37.302846402707324	39.0	35.0	41.0	32.0	41.0
16	37.3785433588121	39.0	35.0	41.0	32.0	41.0
17	37.27647613102122	39.0	35.0	41.0	32.0	41.0
18	37.168618857857275	39.0	35.0	41.0	32.0	41.0
19	37.0939683610297	38.0	35.0	41.0	32.0	41.0
20	36.9186945912156	38.0	35.0	41.0	32.0	41.0
21	36.74629492327081	38.0	35.0	41.0	31.0	41.0
22	36.676358723395	38.0	35.0	40.0	31.0	41.0
23	36.61123891405896	38.0	35.0	40.0	31.0	41.0
24	36.521403669678705	38.0	35.0	40.0	31.0	41.0
25	36.417177831967344	38.0	35.0	40.0	31.0	41.0
26	36.30448575794447	38.0	35.0	40.0	31.0	41.0
27	36.23022104098639	38.0	35.0	40.0	30.0	41.0
28	36.22974697526825	38.0	35.0	40.0	30.0	41.0
29	36.23067795163442	38.0	35.0	40.0	30.0	41.0
30	36.21304379480193	38.0	35.0	40.0	30.0	41.0
31	36.08408453014355	38.0	35.0	40.0	30.0	41.0
32	35.90477262419016	38.0	35.0	40.0	30.0	41.0
33	35.71989452560792	38.0	35.0	40.0	29.0	41.0
34	35.61002843557476	38.0	35.0	40.0	29.0	41.0
35	35.37533243178558	38.0	34.0	40.0	27.0	41.0
36	35.27728576664753	38.0	34.0	40.0	26.0	41.0
37	35.236409837135625	38.0	34.0	40.0	27.0	41.0
38	35.1621551621698	38.0	34.0	40.0	26.0	41.0
39	35.11994448451943	38.0	34.0	40.0	26.0	41.0
40	35.069741636275694	38.0	34.0	40.0	25.0	41.0
41	35.0183069703142	38.0	34.0	40.0	25.0	41.0
42	34.98586882480238	38.0	34.0	40.0	25.0	41.0
43	34.92991609915463	37.0	34.0	40.0	25.0	41.0
44	34.80243719152255	37.0	34.0	40.0	24.0	41.0
45	34.72044892726418	37.0	34.0	40.0	24.0	41.0
46	34.6850107365634	37.0	34.0	40.0	24.0	41.0
47	34.632409525833864	37.0	34.0	40.0	24.0	41.0
48	34.497476112610904	37.0	34.0	40.0	23.0	41.0
49	34.441615020142564	37.0	34.0	40.0	24.0	41.0
50	34.34633701595505	36.0	33.0	40.0	24.0	41.0
51	33.93699695895001	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	4.0
13	4.0
14	16.0
15	45.0
16	116.0
17	364.0
18	867.0
19	1758.0
20	3244.0
21	5390.0
22	8373.0
23	13035.0
24	19315.0
25	28650.0
26	38930.0
27	44337.0
28	47295.0
29	51976.0
30	61025.0
31	73414.0
32	90999.0
33	117514.0
34	191928.0
35	292934.0
36	179571.0
37	216352.0
38	324146.0
39	578203.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.803476537721906	24.220239300675658	29.299604512871326	15.67667964873111
2	31.625497287825254	25.965621239483106	29.679903128247958	12.72897834444368
3	28.0120118964135	25.327661839257832	33.382887775715446	13.277438488613216
4	24.614136447243556	27.881173105536316	33.31677799330869	14.187912453911439
5	22.55904273035075	32.014373438260996	31.820270096118602	13.606313735269653
6	20.646419778707966	41.05459329094497	27.90426968774425	10.394717242602818
7	87.28570806924289	3.420930189743444	7.919784565792623	1.3735771752210495
8	87.98145076662243	2.709245829644296	7.538481751189558	1.7708216525437204
9	83.43251195415495	4.949823511985954	9.144112630985237	2.473551902873851
10	43.463541710251704	30.001539772147197	16.005471212118678	10.529447305482426
11	33.457365885009146	23.643619736531598	27.612089554487014	15.286924823972244
12	32.36944154807353	22.527535979621447	28.683946703799723	16.4190757685053
13	21.185130822054223	33.26987352110743	29.25872523602866	16.286270420809686
14	16.646526893292116	35.41915275711266	30.05237735798531	17.881942991609915
15	15.473831404991875	27.64062554917145	41.496440950574986	15.389102095261686
16	17.43796140862373	24.669911345944957	40.505882096968826	17.386245148462486
17	17.775121298898224	26.008299706606458	29.599692715036714	26.6168862794586
18	20.380432508606823	27.1936732101404	33.788082163580704	18.637812117672066
19	24.72397073763454	28.97646157013244	28.004856976925176	18.294710715307847
20	26.367342771690282	26.84613659452611	29.31960481413109	17.466915819652513
21	21.393125586828923	29.60078059753201	30.523179428644116	18.48291438699495
22	21.337225163224215	26.20591774604136	29.821369694271542	22.635487396462874
23	18.388728867882527	31.132435467647213	28.762274243461405	21.71656142100885
24	19.087442321306934	26.93107511242847	36.56716168109645	17.414320885168145
25	17.628340845301434	29.424962049637564	33.194935153834955	19.751761951226044
26	16.976071606099506	34.60562585879955	29.32583921766186	19.09246331743909
27	17.77227606775667	33.51657179773419	30.69284725627666	18.018304878232474
28	16.479578771897817	30.359704162907892	36.28113226810111	16.879584797093177
29	16.662133822936244	26.99902592675036	35.64480469161879	20.69403555869461
30	18.76865090854925	31.29607809992117	32.29952417693321	17.63574681459637
31	24.412501610902925	29.337764083475736	28.451349057977442	17.798385247643896
32	24.701250730136522	29.167719681133274	28.91030994609124	17.22071964263897
33	22.893650280924735	30.52430915277385	27.963935858448075	18.618104707853337
34	18.76936221633464	29.396384213318694	29.74262373826551	22.091629832081153
35	19.10330030075767	29.18073242944245	31.471352706567966	20.244614563231913
36	24.23191311668293	28.849388526354367	28.650766287693035	18.267932069269662
37	18.898778391641045	32.9171067011888	30.29736012760025	17.886754779569902
38	18.248768600698586	33.11903442897048	28.109126329936352	20.523070640394582
39	18.709988937071856	32.026549353881485	29.73412988647528	19.529331822571386
40	21.825893611786622	29.586805491630834	27.935190655591462	20.652110240991078
41	17.21812546130402	27.900754990451738	30.130746739281427	24.750372808962812
42	20.289385112077003	27.95134152648324	27.56878346284714	24.190489898592617
43	20.415161065187593	29.040646637355206	27.861716745524202	22.682475551933
44	18.01985301870656	32.27630206982197	29.27550373143696	20.42834118003451
45	17.429049140489145	35.217434237503156	26.986933694398747	20.36658292760895
46	20.615917227205095	33.3476989611559	26.689104940492825	19.347278871146177
47	19.682472204602245	29.650446617605958	28.702357022950974	21.964724154840827
48	20.569975112595838	27.360956064610175	32.011026107506225	20.058042715287762
49	19.67945960692295	27.76799148439056	31.026074032914302	21.526474875772188
50	17.9938275220882	31.87031269090246	29.612538096808155	20.52332169020119
51	17.522230460375134	31.72641931008166	28.08523475667416	22.66611547286905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1396.0
1	2026.0
2	2656.0
3	18903.0
4	35150.0
5	24296.5
6	13443.0
7	13318.5
8	13194.0
9	14512.5
10	15831.0
11	16916.0
12	18001.0
13	18166.5
14	18332.0
15	17200.5
16	16069.0
17	15337.0
18	14605.0
19	14516.0
20	14427.0
21	13885.0
22	13343.0
23	14478.5
24	15614.0
25	18249.0
26	24629.0
27	28374.0
28	33691.0
29	39008.0
30	46510.0
31	54012.0
32	63600.0
33	73188.0
34	81492.0
35	89796.0
36	92821.0
37	95846.0
38	108137.5
39	120429.0
40	147158.0
41	173887.0
42	193333.0
43	212779.0
44	219878.5
45	226978.0
46	225482.5
47	223987.0
48	214830.0
49	205673.0
50	188389.5
51	171106.0
52	152030.5
53	132955.0
54	116650.5
55	100346.0
56	86926.5
57	73507.0
58	64765.5
59	56024.0
60	47316.0
61	38608.0
62	32146.0
63	25684.0
64	20651.0
65	15618.0
66	11850.5
67	8083.0
68	6509.5
69	4936.0
70	3812.0
71	2688.0
72	2159.0
73	1630.0
74	1242.5
75	725.5
76	596.0
77	428.5
78	261.0
79	175.0
80	89.0
81	61.5
82	34.0
83	29.0
84	24.0
85	15.5
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2389964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.589696482869016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8289156773846	16.587129705826705
2	8.462319378945454	3.65397813865066
3	3.072915567696983	1.9902994327218297
4	1.7037006776605388	1.4712952211339718
5	1.062815302104691	1.1472929894894512
6	0.7347722808955427	0.9518106317136087
7	0.5251872120132147	0.793704275283464
8	0.44411528309450676	0.767065133233108
9	0.3727278163041326	0.7242372382255903
>10	4.407124032136281	22.69326913756519
>50	1.6226030024305658	25.174773761898233
>100	0.7574928693924105	22.064996205130257
>500	0.004130699954216273	0.6265146553364007
>1k	9.834999890991126E-4	0.33119960665414416
>5k	0.0	0.0
>10k+	1.9669999781982253E-4	1.022433867137476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24076	1.0073791906488967	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3103	0.1298345916507529	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.368326886932189E-5	0.0	0.0	0.04874550411638	0.0
2	8.368326886932189E-5	0.0	0.0	0.16075555949796733	0.0
3	8.368326886932189E-5	0.0	0.0	0.28180340791744146	0.0
4	8.368326886932189E-5	0.0	0.0	0.4798398636966917	0.0
5	8.368326886932189E-5	0.0	0.0	0.8629000269460125	0.0
6	8.368326886932189E-5	0.0	0.0	1.4491013253756124	0.0
7	8.368326886932189E-5	0.0	0.0	1.7636248914209587	0.0
8	8.368326886932189E-5	0.0	0.0	2.477694224682882	0.0
9	8.368326886932189E-5	0.0	0.0	2.781171599237478	0.0
10	8.368326886932189E-5	0.0	0.0	3.2349022830469414	0.0
11	8.368326886932189E-5	0.0	0.0	3.709051684460519	0.0
12	8.368326886932189E-5	0.0	0.0	4.095626545002352	0.0
13	8.368326886932189E-5	0.0	0.0	4.294918249814642	0.0
14	8.368326886932189E-5	0.0	0.0	4.388225094603936	0.0
15	8.368326886932189E-5	0.0	0.0	4.500904616136477	0.0
16	8.368326886932189E-5	0.0	0.0	4.707016507361617	0.0
17	8.368326886932189E-5	0.0	0.0	4.954760824849244	0.0
18	8.368326886932189E-5	0.0	0.0	5.259702656609053	0.0
19	8.368326886932189E-5	0.0	0.0	5.453345740772664	0.0
20	8.368326886932189E-5	0.0	0.0	5.636779466134218	0.0
21	8.368326886932189E-5	0.0	0.0	5.8827245933411545	0.0
22	8.368326886932189E-5	0.0	0.0	6.158921222244352	0.0
23	8.368326886932189E-5	0.0	0.0	6.468423792157539	0.0
24	8.368326886932189E-5	0.0	0.0	6.689431305241418	0.0
25	8.368326886932189E-5	0.0	0.0	6.897844486360464	0.0
26	8.368326886932189E-5	0.0	0.0	7.0933286024391995	0.0
27	8.368326886932189E-5	0.0	0.0	7.290904800239669	0.0
28	8.368326886932189E-5	0.0	0.0	7.506347375943738	0.0
29	8.368326886932189E-5	0.0	0.0	7.737731614367413	0.0
30	8.368326886932189E-5	0.0	0.0	8.028572815322741	0.0
31	8.368326886932189E-5	0.0	0.0	8.27443425926081	0.0
32	8.368326886932189E-5	0.0	0.0	8.504856140092487	0.0
33	8.368326886932189E-5	0.0	0.0	8.745863954436134	0.0
34	8.368326886932189E-5	0.0	0.0	8.987373868392996	0.0
35	8.368326886932189E-5	0.0	0.0	9.269972267364697	0.0
36	8.368326886932189E-5	0.0	0.0	9.530394600086026	0.0
37	8.368326886932189E-5	0.0	0.0	9.796758444897078	0.0
38	8.368326886932189E-5	0.0	0.0	10.05262003946503	0.0
39	8.368326886932189E-5	0.0	0.0	10.333210039983866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTA	20	7.034816E-4	45.000004	42
ACACGTC	25	3.892074E-5	45.0	15
CTATACG	50	2.1827873E-11	45.0	1
GTTAGCG	125	0.0	44.999996	1
CATACCG	30	2.1664891E-6	44.999996	21
CGTTTTT	12105	0.0	43.289963	1
TTTAGCG	100	0.0	42.75	1
CGTAAGG	495	0.0	41.81818	2
TCGCGAG	120	0.0	41.249996	1
GCGTAAG	255	0.0	40.588234	1
TGTAGCG	190	0.0	40.26316	1
TATAGCG	185	0.0	40.135136	1
CGGGTAC	225	0.0	40.0	6
TAGTACG	90	0.0	40.0	1
CATACGA	815	0.0	39.7546	18
CTTCGCG	40	3.460318E-7	39.375004	1
CGATCTA	80	0.0	39.375004	10
ACGTAGG	390	0.0	39.23077	2
CGAGGGA	1355	0.0	39.022137	4
AGGGATC	2450	0.0	38.84694	6
>>END_MODULE