FastQCFastQC Report
Thu 26 May 2016
SRR1547635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547635_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1517480
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76300.5028072857632391No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG38280.2522603263304953No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC34240.2256372406885099No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC28030.18471413132298284No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20190.1330495294830904No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT207.0340006E-445.00000439
GCGATCG207.0340006E-445.0000049
GCGTATT207.0340006E-445.00000416
CCGCAAT207.0340006E-445.00000433
CGCATCG253.8913982E-545.021
TCCGTAA253.8913982E-545.028
TTACGCG253.8913982E-545.01
TAAGTCG302.1659635E-644.9999961
TATAGCG600.044.9999961
TACCGAT302.1659635E-644.99999612
TTAATCG1700.042.3529420
GTTAGCG750.042.01
CGTTTTT47100.041.9904481
GGGCGAT20450.041.2591677
ACCCGCT1050.040.71428734
CGGTCTA1900.040.26315731
CGCACTC451.9290383E-840.032
TAATCGT1800.040.021
TACGGGA6200.039.556454
AATGCGG2000.039.3752